Acoustic Wave-Based Detection in Bioanalytical Chemistry: Competition for Surface Plasmon Resonance?

Sheikh, Sonia; Blaszykowski, Christophe; Thompson, Michael

doi:10.1080/00032710802368106

Cited by 21 publications

(10 citation statements)

References 53 publications

(48 reference statements)

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“…Based on a 10:1 signal-to-noise criterion, C d measurements demonstrated limits of quantification down to 3 × 10 10 targets cm −2 , corresponding to several pg of material per mm 2 . This performance matches that of other label-free methods, including surface plasmon resonance69 and quartz crystal microbalance71 techniques, that could be used for analysis of nucleic acids by surface hybridization. In the case of capacitive transduction, sensitivity is expected to improve at lower ionic strengths, under conditions more dilute than the 0.2 mol L −1 phosphate buffer used.…”

Section: Discussionsupporting

confidence: 72%

Morpholino Monolayers: Preparation and Label-free DNA Analysis by Surface Hybridization

et al. 2009

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Surface hybridization, a reaction in which nucleic acid molecules in solution react with nucleic acid partners immobilized on a surface, is widely practiced in life science research. In these applications the immobilized partner, or "probe", is typically single-stranded DNA. Because DNA is strongly charged, high salt conditions are required to enable binding between analyte nucleic acids ("targets") in solution and the DNA probes. High salt, however, compromises prospects for label-free monitoring or control of the hybridization reaction through surface electric fields, as well as stabilizes secondary structure in target species that can interfere with probe-target recognition. In this work, initial steps toward addressing these challenges are taken by introducing Morpholinos, a class of uncharged DNA analogues, for surface-hybridization applications. Monolayers of Morpholino probes on gold supports can be fabricated with methods similar to those employed with DNA, and are shown to hybridize efficiently and sequence-specifically with target strands. Hybridization-induced changes in the interfacial charge organization are analyzed with electrochemical methods and compared for Morpholino and DNA probe monolayers. Molecular mechanisms connecting surface hybridization state to the interfacial capacitance are identified and interpreted through comparison to numerical Poisson Boltzmann calculations. Interestingly, positive as well as negative capacitive responses (contrast inversion) to hybridization are possible, depending on surface populations of mobile ions as controlled by the applied potential. Quantitative comparison of surface capacitance with target coverage (targets/area) reveals a near-linear relationship, and demonstrates sensitivities (limits of quantification) in the pg mm −2 range.

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Section: Discussionsupporting

confidence: 72%

Morpholino Monolayers: Preparation and Label-free DNA Analysis by Surface Hybridization

et al. 2009

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“…Transducers may be electrochemical, optical and gravimetric (acoustic wave (AW)/quartz crystal microbalance (QCM)). The techniques have been explained in several reviews [ 103 , 104 , 105 , 106 , 107 , 108 ]. The most commonly used immunosensors are based on optical transducers and surface plasmon resonance (SPR) is the most known and utilized.…”

Section: Immunosensorsmentioning

confidence: 99%

Immunochemical Methods for Ochratoxin A Detection: A Review

Meulenberg¹

2012

Toxins

110

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The safety of food and feed depends to a great deal on quality control. Numerous compounds and organisms may contaminate food and feed commodities and thus pose a health risk for consumers. The compound of interest in this review is ochratoxin A (OTA), a secondary metabolite of the fungi Aspergillus and Penicillium . Due to its adverse health effects, detection and quantification are of utmost importance. Quality control of food and feed requires extraction and analysis, including TLC, HPLC, MS, and immunochemical methods. Each of these methods has its advantages and disadvantages. However, with regard to costs and rapidity, immunochemical methods have gained much interest in the last decade. In this review an introduction to immunochemistry and assay design will be given to elucidate the principles. Further, the application of the various formats to the detection and quantification of ochratoxin will be described, including the use of commercially available kits.

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“…[497][498][499][500] Silane monolayers have found more applications in the domains of molecular electronics 501 or of sensors whose transduction mechanism is based on physical principles (e.g., surface acoustic wave) rather than on electrochemical ones. 502,503 The electrode surface can be modified by silanes via the sol-gel chemistry route, which leads to the formation of multimolecular layers. The environmental applications of such chemically modified electrodes will be presented in a following section.…”

Section: Other Types Of Monolayers On Electrodesmentioning

confidence: 99%