ACLIMATACION DE Uncaria tomentosa (Willd.) DC. PRODUCIDA IN VITRO

Torrejón, Gilberto Domínguez; Gómez, María Luz Donayre

doi:10.21704/rea.v5i1-2.319

Cited by 2 publications

(1 citation statement)

References 2 publications

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“…Domínguez-Torrejón and Donayre-Gómez (2006) suggest taking the in vitro cultures to a greenhouse to start conditioning because the plants obtained under these conditions suffer relatively less stress when they are transferred from in vitro to in vivo than the plants obtained from cultures incubated in the laboratory.…”

Section: Resultsmentioning

confidence: 99%

Effect of Culture Medium and Incubation on the Characteristics of Micropropagated Agave Plants

2016

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. The shoots in each medium were separated into two groups and incubated for 84 days under different environments, including a laboratory or greenhouse. The experimental design was completely randomized with a 3×3×2 factorial arrangement. Adventitious roots emerged after 10 to 14 days of incubation, depending on the condition of incubation. In both laboratory and greenhouse settings, after 28 days of incubation, 66.6 and 93.6% of shoots subjected to treatment medium with 75% inorganic salts, 0.5 mg L -1 IBA and medium with 50% inorganic salts, 1 mg L -1 IBA, respectively, had formed roots. On day 40, all shoots had roots. After the 84-day rooting stage, the plants grown from the shoots in the medium with 1 mg L -1 IBA were larger in terms of leaf width, stem diameter, and largest leaf base cross-section area, but similar in the number of roots relative to the plants obtained in the media without IBA. Plant size increased with increasing mineral salt concentrations in the medium. Micropropagated plants obtained from the cultures incubated in a greenhouse and laboratory had an 8.9 and 5.4 cm leaf length, 1.8 and 0.8 cm 3 leaf volume, 1.8 and 0.5 cm 3 stem diameter, and 6.6 and 5.2 roots, respectively.

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Section: Resultsmentioning

confidence: 99%

Effect of Culture Medium and Incubation on the Characteristics of Micropropagated Agave Plants

2016

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An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (Fragaria × ananassa Duch.) Variety ‘Aroma’

et al. 2022

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In strawberry micropropagation, several challenges must be overcome to obtain quality plants and achieve high survival rate to ex vitro acclimatization. In this study, therefore, a set of protocols were evaluated to optimize explant (meristem) disinfection, in vitro growth (multiplication and rooting), and ex vitro acclimatization of strawberry. The results showed that explants treated with 1.0% NaClO for 5 min had a lower percentage of contamination, and achieved a higher percentage of viability, height, and number of leaves. In vitro growth was favored by the use of 1 mg L−1 zeatin, since it allowed greater seedling growth (number of shoots, seedling height, number of leaves, number of roots and root length), and a higher pre-acclimation rate (100%). In the acclimatization phase, plants grown in a substrate composed of compost + peat combined with 4 g of humic acid achieved better response in morphological and physiological variables. In fact, the results of this study could be used to cultivate strawberry plants of the ‘Aroma’ variety with high commercial quality.

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ACLIMATACION DE Uncaria tomentosa (Willd.) DC. PRODUCIDA IN VITRO

Cited by 2 publications

References 2 publications

Effect of Culture Medium and Incubation on the Characteristics of Micropropagated Agave Plants

Effect of Culture Medium and Incubation on the Characteristics of Micropropagated Agave Plants

An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (Fragaria × ananassa Duch.) Variety ‘Aroma’

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