Acid Glycosaminoglycans in Myxoedema

Parving, Hans‐Henrik; Helin, G.; Garbarsch, C.; Johansen, Å.; Jensen, Bente; Hélin, P; Lund, P. A.; Lyngsøe, J.

doi:10.1111/j.1365-2265.1982.tb03166.x

Cited by 24 publications

(10 citation statements)

References 9 publications

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“…We also examined whether T 3 has direct actions on these cells by investigating the effects of T 3 on hyaluronic acid (HA) metabolism, which is regulated by these hormones in a number of thyroid hormone target tissues [16]. The results indicate that TRs are present and functional in these cells, indicating that this tissue is a direct target for thyroid hormones.…”

Section: Introductionmentioning

confidence: 99%

The Human Fetal Retinal Pigment Epithelium: A Target Tissue for Thyroid Hormones

et al. 1999

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Thyroid hormone (T₃) has previously been shown to regulate visual function in experimental animals and humans. To determine if T₃ exerts direct effects on retinal function, cultured human fetal retinal pigment epithelial (RPE) cells were tested for the presence of thyroid hormone receptors (TRs) and T₃ responses. Using TR-isoform-specific reverse-transcriptase polymerase chain reaction techniques, mRNA was detected for α1, α2 and β1 TR isoforms. Immunohistochemistry using a polyclonal antibody that simultaneously recognizes α1, α2 and β1 TRs showed nuclear staining of the fetal RPE. Specific binding of ¹²⁵I-T₃ to RPE cell nuclear extracts was detected, and Scatchard analysis revealed a K_d of 110 pM. To determine if RPE cells can respond to T₃, hyaluronic acid (HA) levels in cell culture media were measured after 2, 4 or 6 days of growth in medium containing 10^–7 M T₃. T₃ inhibited accumulation of HA in the cell culture medium of RPE cells. This effect was not evident at 2 days, but at 4 days there was 42.8% less HA in cell culture medium of RPE cells grown in 10^–7 M T₃ (p < 0.01, t test). The effect persisted through 6 days, when there was 46.3% less HA in cell culture medium of RPE cells grown in 10^–7 M T₃ (p < 0.001, t test). The data indicate that human fetal RPE cells are a direct target for thyroid hormones.

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Section: Introductionmentioning

confidence: 99%

The Human Fetal Retinal Pigment Epithelium: A Target Tissue for Thyroid Hormones

et al. 1999

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“…Eight of 11 control dogs (Case Nos. 9, 10, and [12][13][14][15][16][17] also showed alcianophilic deposits in the periadnexal areas, but the degree of the reaction was milder than in hypothyroid dogs (at most graded +); in these animals, the reaction was seldom positive in the two other areas (2 of 11 [Case Nos. 13 and 171) and 3 of 11 [Case Nos.…”

Section: Resultsmentioning

confidence: 99%

“…7-12), ranging from 1 to 3 years of age, and two male and three female mongrel dogs (Case Nos. [13][14][15][16][17], ranging from 5 to 14 years of age, were used as controls. They were cared for and used according to current E.E.C.…”

Section: Methodsmentioning

confidence: 99%

“…This description was made on the basis of the histological demonstration of a jellylike interstitial material (mucin) in the ~k l n .~ Myxedema now refers in human medicine to the generalized mucinosis of the skin accompanying hyperthyroidism ("pretibial myxedema") or hypothyroidism ("generalized m y~e d e m a " ) .~J O .~~,~~ Histochemical and biochemical studies showed that the accumulated mucin mainly consists of hyaluronic a~i d ;~~~~~~~~~~~~~ this highly hygroscopic GAG might contribute to the formation of the macroscopical cutaneous edema. 14,23,24 In canine hypothyroidism, the occasional presence of a dermal mucinosis, either as cutaneous thickening9J2 or, rarely, as cutaneous vesiculation," has been reported. The topographical and histochemical characterization of this mucinosis has so far not been studied in this species.…”

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confidence: 99%

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Histochemical Study of Cutaneous Mucins in Hypothyroid Dogs

Doliger

Delverdier

Moré³

et al. 1995

Vet Pathol

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Abstract. A dermal mucinoisis, visualized as dermal alcianophilic material, is occasionally present in canine hypothyroidism (myxedema). Various histochemical reactions (alcian blue/periodic acid-Schiff [PAS], alcian blue at pH 2.6, alcian blue at pH 1.0, critical electrolytical concentrations with and without dimethylsulfoxide, differential hydrolysis by hyaluronidases) were performed on skin biopsies from six dogs (four females and two males ranging from 8 to 13 years) affected by hypothyroidism, all of them presenting dermal mucinosis in hematoxylin and eosin-stained sections. In these dogs, the only polysaccharidic compound involved in the dermal mucinosis was hyaluronic acid. In this study, hyaluronic acid dermal deposits of hypothyroid dogs were significantly different from those of controls in subepidermal connective tissue and loose reticular connective tissue but not in periadnexal zones. We recommend the combined alcian blue/PAS reaction as a routine technique to assess dermal mucinosis in hypothyroid dogs.

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“…This is due to decreased camerular filtration, although part of the heparan sul-tabolism of hyaluronic acid and an increased rate of phate fraction may originate from renal tissue (2,3). synthesis of sulphated glycosaminoglycans (5,6). Because urinary glycosaminoglycans are derived from ^ , , , ,.…”

Section: Introductionmentioning

confidence: 98%

Urinary Glycosaminoglycans in Patients with Hypothyroidism and in Healthy Subjects

Wlad

Fenrych²,

Łącka³

et al. 1988

Clinical Chemistry and Laboratory Medicine

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Although the changes in urinary glycosaminoglycans have been investigated in several endocrinopathies, no information was hitherto available on the content and composition of urinary glycosaminoglycans in hypothyroidisin. Urinary glycosaminoglycans were therefore investigated in patients with hypo thy roidism and in healthy subjects. The total daily excretion of urinary glycosaminoglycans was found to be significantly increased (by 41 %) in hypothyroidism. Two electrophoretic bands were always detected in both examined groups: a major band of chondroitin sulphate and a minor band of heparan sulphate. Heparan sulphate and chondroitin sulphate levels were respectively 114% and 42% higher in patients with hypothyroidism than in controls. The respective increases in chondroitin-4-sulphate and chondroitin-6-sulphate were 31% and 41%. The relative quantities of chondroitin-4-sulphate, dermatan sulphate, chondroitin-6-sulphate and non-sulphated chondroitin sulphate were unchanged in the two examined groups. The changes observed in the levels of the excreted glycosaminoglycans may reflect the altered metabolism of connective tissue in hypothyroidism.

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Acid Glycosaminoglycans in Myxoedema

Cited by 24 publications

References 9 publications

The Human Fetal Retinal Pigment Epithelium: A Target Tissue for Thyroid Hormones

The Human Fetal Retinal Pigment Epithelium: A Target Tissue for Thyroid Hormones

Histochemical Study of Cutaneous Mucins in Hypothyroid Dogs

Urinary Glycosaminoglycans in Patients with Hypothyroidism and in Healthy Subjects

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