Histochemical Study of Cutaneous Mucins in Hypothyroid Dogs

Doliger, S.; Delverdier, Maxence; Moré, J.; Longeart, L.; Régnier, A.; Magnol, J. P.

doi:10.1177/030098589503200603

Cited by 19 publications

(12 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Positive alcian blue staining indicates the presence of acid mucus polysaccharides (Doliger et al, 1995; Sasai, 1971). To further characterize the swollen connective tissues of bulgy morph tadpoles, sections were treated with 1% alcian blue 8GS-1N HCl (pH 1.0, for strongly sulfated glycosaminoglycans) or 1% alcian blue 8GS-3% acetic acid (pH 2.5, for a broad range of acidic glycosaminoglycans).…”

Section: Methodsmentioning

confidence: 99%

Histological and MS spectrometric analyses of the modified tissue of bulgy form tadpoles induced by salamander predation

et al. 2012

View full text Add to dashboard Cite

SummaryThe rapid induction of a defensive morphology by a prey species in face of a predation risk is an intriguing in ecological context; however, the physiological mechanisms that underlie this phenotypic plasticity remain uncertain. Here we investigated the phenotypic changes shown by Rana pirica tadpoles in response to a predation threat by larvae of the salamander Hynobius retardatus. One such response is the bulgy morph phenotype, a relatively rapid swelling in size by the tadpoles that begins within 4 days and reaches a maximum at 8 to 10 days. We found that although the total volume of bodily fluid increased significantly (P<0.01) in bulgy morph tadpoles, osmotic pressure was maintained at the same level as control tadpoles by a significant increase (P<0.01) in Na and Cl ion concentrations. In our previous report, we identified a novel frog gene named pirica that affects the waterproofing of the skin membrane in tadpoles. Our results support the hypothesis that predator-induced expression of pirica on the skin membrane causes retention of absorbed water. Midline sections of bulgy morph tadpoles showed the presence of swollen connective tissue beneath the skin that was sparsely composed of cells containing hyaluronic acid. Mass spectrographic (LC-MS/MS) analysis identified histone H3 and 14-3-3 zeta as the most abundant constituents in the liquid aspirated from the connective tissue of bulgy tadpoles. Immunohistochemistry using antibodies against these proteins showed the presence of non-chromatin associated histone H3 in the swollen connective tissue. Histones and 14-3-3 proteins are also involved in antimicrobial activity and secretion of antibacterial proteins, respectively. Bulgy tadpoles have a larger surface area than controls, and their skin often has bite wounds inflicted by the larval salamanders. Thus, formation of the bulgy morph may also require and be supported by activation of innate immune systems.

show abstract

Section: Methodsmentioning

confidence: 99%

Histological and MS spectrometric analyses of the modified tissue of bulgy form tadpoles induced by salamander predation

et al. 2012

View full text Add to dashboard Cite

show abstract

“…In acromegaly the skin thickening has been ascribed to mucopolysaccharide accumulation (Matsuoka et al 1982). Similarly, in dogs with primary hypothyroidism the thickening of the skin is also due to mucopolysaccharide accumulation (Muller et al 1989, Doliger et al 1995. Thus, some of the physical changes observed in dogs with primary hypothyroidism might very well be acromegalic changes.…”

Section: Dogmentioning

confidence: 99%

Primary hypothyroidism in dogs is associated with elevated GH release

Diaz-Espineira²,

Mol³

et al. 2001

Journal of Endocrinology

View full text Add to dashboard Cite

The pulsatile secretion patterns of GH were investigated in seven beagle bitches by collecting blood samples every 10 min for 6 h during euthyroidism and 1·5 years after induction of primary hypothyroidism. Hypothyroidism was induced by surgical removal of the thyroid gland and subsequent destruction of any remnant thyroid tissue by oral administration of sodium [131 I]iodide. Some of the physical changes observed in the dogs with primary hypothyroidism mimicked those of acromegaly. During both euthyroidism and hypothyroidism GH was secreted in a pulsatile fashion. The mean ( ...) basal plasma GH concentration was significantly higher (P=0·003) in the hypothyroid state (4·1 1·6 µg/l) than in the euthyroid state (1·2 0·4 µg/l). Likewise, the mean area under the curve (AUC) for GH above the zero-level during hypothyroidism (27·0 10·0 µg/l 6 h) was significantly higher (P=0·004) than that during euthyroidism (11·7 2·0 µg/l 6 h). The mean AUC for GH above the baseline was significantly lower (P=0·008) during hypothyroidism (2·4 0·8 µg/l 6 h) than during euthyroidism (4·5 1·8 µg/l 6 h), whereas there was no significant difference in GH pulse frequency. The mean plasma IGF-I level was significantly higher (P<0·01) in the hypothyroid state (169 45 µg/l) than in the euthyroid (97 15 µg/l). The results of this study demonstrate that primary hypothyroidism in dogs is associated with elevated basal GH secretion and less GH secreted in pulses. This elevated GH secretion has endocrine significance as illustrated by elevated plasma IGF-I levels and some physical changes mimicking acromegaly. It is discussed that the increased GH release in hypothyroid dogs may be the result of the absence of a response element for thyroid hormone within the canine pituitary GH gene and alterations in supra-pituitary regulation.

show abstract

“…One histochemical investigation 5 demonstrated that the mucinous material deposited in the skin of hypothyroid dogs was Alcian blue (AB)/Schiff's periodic acid (PAS)‐positive corresponding to acid glycosaminoglycans (GAGs), concluding that probably the only polysaccharide compound involved in canine dermal mucinosis was hyaluronic acid (HA). Another study 6 demonstrated that in the dermis of shar‐pei dogs with cutaneous mucinosis, the predominant mast cells were chymase‐positive, instead of the more common tryptase‐positive, suggesting that these cells might play a role in the pathogenesis of this disease.…”

Section: Introductionmentioning

confidence: 99%

Cutaneous mucinosis in shar‐pei dogs is due to hyaluronic acid deposition and is associated with high levels of hyaluronic acid in serum

Zanna¹,

Fondevila²,

Bardagí³

et al. 2008

Veterinary Dermatology

View full text Add to dashboard Cite

Cutaneous mucinosis affects primarily shar-pei dogs. Hyaluronic acid (HA) is considered to be the main component of mucin and CD44 is the major cell surface receptor of HA, necessary for its uptake and catabolism. The aims of this study were to identify the composition of the mucin in cutaneous mucinosis of shar-pei dogs, investigate the correlation between the deposition of HA and the expression of CD44, and determine whether shar-pei dogs with cutaneous mucinosis presented with elevated levels of serum HA. In skin biopsies, the mucinous material was stained intensely with Alcian blue and bound strongly by the hyaluronan-binding protein. No correlation was found between the degree of HA deposition in the dermis and the expression of CD44 in the skin of shar-pei dogs affected or unaffected by cutaneous mucinosis. A clear positive correlation was found between the existence of clinical mucinosis and the serum HA concentration. In control dogs, serum HA ranged from 155.53 to 301.62 microg L(-1) in shar-pei dogs; without mucinosis it ranged from 106.72 to 1251.76 microg L(-1) and in shar-pei dogs with severe mucinosis it ranged between 843.51 to 2330.03 microg L(-1). Altogether, the results reported here suggest that mucinosis of shar-pei dogs is probably the consequence of a genetic defect in the metabolism of HA.

show abstract

Histochemical Study of Cutaneous Mucins in Hypothyroid Dogs

Cited by 19 publications

References 14 publications

Histological and MS spectrometric analyses of the modified tissue of bulgy form tadpoles induced by salamander predation

Histological and MS spectrometric analyses of the modified tissue of bulgy form tadpoles induced by salamander predation

Primary hypothyroidism in dogs is associated with elevated GH release

Cutaneous mucinosis in shar‐pei dogs is due to hyaluronic acid deposition and is associated with high levels of hyaluronic acid in serum

Contact Info

Product

Resources

About