Acid and neutral  -glucosidase in the reproductive organs and seminal plasma of the bull

Jauhiainen, Anneli; Vanha‐Perttula, T.

doi:10.1530/jrf.0.0740669

Cited by 16 publications

(18 citation statements)

References 13 publications

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“…In the present study, activity of α‐glucosidase recovered along with restoration of epididymal androgens in ethanol‐withdrawn rats. α‐glucosidase has also been suggested to play a role in destruction of the cytoplasmic droplets of spermatozoa or in the digestion of adsorbed polysaccharides and glycoproteins, as well as in the processing of glycoproteins synthesized by the epididymis ( Jauhiainen & Vanha‐Pertula, 1985). From these observations, it is suggested that the low activity of sperm α‐glucosidase may be one of the factors responsible for the high incidence of spermatozoa containing cytoplasmic droplets recorded in the present study.…”

Section: Discussionmentioning

confidence: 99%

Effects of ethanol ingestion on epididymal glycosidases and fertility in the rat

et al. 1998

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Epididymal glycosidases play a role in sperm maturation by modifying sperm surface glycoproteins. To study the effects of ethanol on epididymal sperm maturation, ethanol (3 g/kg body weight as 25%, v/v) was administered to a group of rats by gastric-intubation twice daily for 30 days. In another group, rats were also treated with alcohol for 30 days but were then withdrawn from treatment for 30 days to assess the reversibility of ethanol-induced effects. Ethanol-induced changes in epididymal tissue and sperm glycosidases, cauda epididymal sperm motility and the fertility of rats were assessed. Ethanol treatment caused a marked decrease in the specific activities of glycosidases in both tissues and spermatozoa from epididymal segments. Cauda epididymal sperm motility and the fertility of ethanol-treated rats were significantly impaired compared to control rats fed an isocaloric diet. These changes are likely to be the consequence of direct and indirect effects of ethanol mediated through subnormal testosterone and dihydrotestosterone. Most of these changes were found to be reversible. The present study suggests that impaired activity of sperm glycosidases may be one of the factors responsible for defective sperm motility and fertilizing potential in ethanol-treated rats.

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Section: Discussionmentioning

confidence: 99%

Effects of ethanol ingestion on epididymal glycosidases and fertility in the rat

et al. 1998

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“…All the bull semen samples and reproductive or gans were obtained as described previously [21], In studying the ß-glucuronidase distribution in the bull epididymis, the organ was divided into six segments (E|-E6) as indicated earlier [22,23], Segments E1-E3 formed the caput epididymidis, E4 the corpus epididymidis and E5 and E6 the cauda epididymidis.…”

Section: Semen and Reproductive Organsmentioning

confidence: 99%

“…The tissue homogenates, epididymal secretions and sperm samples were prepared as described in detail previously [21].…”

Section: Preparation Of Tissue Homogenatesmentioning

confidence: 99%

“…The enzyme samples (3-4 ml) were eluted with the same buffer and fractions of 2.7 ml were collected at 4"C. Calibration with the marker proteins was done as reported earlier [21], Fractionation with chromatofocusing in pH gra dient was done with Polybuffer 74-HC1 (pH 4.0) as described earlier [21], After the usual Polybuffer elu tion a NaCl gradient (0-0.5 mol/1) was formed in 0.02 mol/1 sodium acetate (pH 5.0). After cooling the samples on ice, the substrate was added and the enzymes were incubated at 37 "C to determine the remaining activities.…”

Section: Chromatographic Proceduresmentioning

confidence: 99%

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ß-Glucuronidase in the Bull Seminal Plasma and Reproductive Organs

Jauhiainen¹,

Vanha‐Perttula²

1986

Enzyme

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The biochemical distribution of ß-glucuronidase activity was studied in different reproductive organs, seminal plasma and spermatozoa of the bull. The highest specific activity was found in the epididymis, where the activity seemed to be mostly in nonsecretory and only partly in secretory form. A molecular weight of 340 X 10^3 to 360 X 10^3 was recorded for ß-glucuronidase in the bull seminal plasma and different reproductive organs with gel filtration on Sepharose 6B. In chromatofocusing four activity areas (CF-1 to CF-4) were usually obtained for ß-glucuronidase in the bull seminal plasma. The major peak CF-2 (also in the different reproductive organs) had a pi value of 5.6-5.3 and the two minor activity areas CF-1 and CF-3 had pi values of 6.0-5,8 and 5.2-4.5, respectively. Peak CF-4 eluted with a NaCl gradient after the Polybuffer elution and possibly represents an enzyme form incompletely detached from negatively charged cellular material. Isoelectric focusing on polyacrylamide gel confirmed the heterogeneity of ß-glucuronidase, since several activity bands were detected in the secretion of the different parts of the epididymis. ß-Glucuronidase activities CF-1, CF-2 and CF-3 had similar pFl activity profiles (pH optimum around pH 3.0-4.0) and response to thermal inactivation at 50 °C. The multiple ß-glucuronidase activities of the bull seminal plasma are proposed to derive mainly from the secretion of the cauda epididymidis.

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“…, 1980;Gonzalez-Eccheverria et al, Eddy et al, 1985;Olson & Hinton, 1985;Brooks & Tiver, 1986;Shabanowitz & Killian, 1987;Holland & Orgebin-Crist, 1988;Hall & Killian, 1989;Cornwall et al, 1990;Ross et al, 1990). However, the existing studies on the proteins responsible for enzymatic activities in epididymal fluid have focused only on certain glycosidases and glycosyltransferases (Hamilton, 1980;Jauhiainen & Vanha-Perttula, 1985;1986;Skudlarek & Orgebin-Crist, 1986), and acid and alkaline phophatases (Nikkanen & Vanha-Perttula, 1977a, b;Mayorga & Bertini, 1982;1985;Miiller, 1983) in the rat, rabbit and bull.…”

Section: Introductionmentioning

confidence: 99%

Selective action of androgens on the molecular forms of esterases characterized by two‐dimensional gel electrophoresis in the epididymis and vas deferens of the mouse

Abou-Haïla¹

1991

Int J of Andrology

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Molecular forms of esterases were resolved in non-denaturing conditions by using two-dimensional gel electrophoresis with isoelectric focusing in the first dimension and a time-dependent polyacrylamide gradient gel electrophoresis (PAGGE) in the second dimension. This procedure was used to analyse sequential changes in esterase composition along the excurrent genital duct of the mouse and to initiate a specific identification of the androgen-regulated molecular forms. Almost all the 68 variants (pH 3.9-6.4 and 50-300 kDa) revealed by alpha-naphtyl acetate from the fluids of the three parts of the epididymis (caput, corpus, cauda) and vas deferens, could be assigned to the carboxylesterase group as shown by their action on various substrates and sensitivity to inhibitors. Some of these variants co-migrated with those in the serum and testis, whereas other enzyme forms made their first appearance in the caput (13), in the corpus (26) and in the vas deferens (3). The major changes occurred between the caput and the corpus of the epididymis. Only a few acidic spots were not revealed after neuraminidase digestion. Castration of mice (4 weeks) resulted in inhibition of the activity of 34 esterase forms, and thus abolished most of the regional differences in the excurrent duct system. By re-initiating or repressing the synthesis of regional esterase variants, testosterone supplementation (2 and/or 4 weeks) of castrated animals restored the normal esterase pattern in the three epididymal parts, but not in the vas deferens. The major effect of efferent duct ligation (4 weeks) was the emergence in the corpus and cauda of the epididymis of two variants found in the caput of uncastrated mice.

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Acid and neutral -glucosidase in the reproductive organs and seminal plasma of the bull

Cited by 16 publications

References 13 publications

Effects of ethanol ingestion on epididymal glycosidases and fertility in the rat

Effects of ethanol ingestion on epididymal glycosidases and fertility in the rat

ß-Glucuronidase in the Bull Seminal Plasma and Reproductive Organs

Selective action of androgens on the molecular forms of esterases characterized by two‐dimensional gel electrophoresis in the epididymis and vas deferens of the mouse

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