ß-Glucuronidase in the Bull Seminal Plasma and
Reproductive Organs

Jauhiainen, Anneli; Vanha‐Perttula, T.

doi:10.1159/000469335

Cited by 6 publications

(2 citation statements)

References 12 publications

(18 reference statements)

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“…The levels of BG were also correlated to sperm concentration, which again suggests a testicular or epididymal origin of this enzyme. In the bull, BG activities are higher in epididymal tissue compared with other reproductive organs and the activities were suggested to be derived mainly from the cauda epididymis (Jauhiainen and Vanha‐Perttula 1986). In man, BG binds with high affinity to the surface of the sperm head (Barbieri et al.…”

Section: Discussionmentioning

confidence: 99%

Alkaline and Acid Phosphatase, β‐Glucuronidase and Electrolyte Levels in Fractionated Stallion Ejaculates

Kareskoski

Reilas

Sankari

et al. 2010

Reprod Domestic Animals

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Seminal plasma (SP) is a mixture of contents from the testes, epididymides and accessory sex glands. The sperm concentration is highest in the first few jets, or fractions, of the ejaculate, and the composition of SP varies between these fractions because accessory gland secretions are released in a specific order. The aim of this study was to compare the levels of Na, Cl, K, Mg, Ca, inorganic phosphate (Pi) and the enzymes alkaline phosphatase (AP), acid phosphatase (ACP) and β-glucuronidase (BG) in the different fractions of the ejaculate and in different stallions. All semen collections were done using a computer-controlled phantom that collects the ejaculatory jets separately in five cups. The cups with the highest (HIGH) and the lowest (LOW) sperm concentration were analysed. In Trial I, semen was collected from three reproductively normal stallions. In Trial II, ejaculates of two reproductively normal stallions were compared to those of two subfertile stallions. In Trial III, semen was collected from seven stallions with varying reproductive history. The sperm-rich fractions contained the highest levels of AP, ACP, BG and inorganic phosphate, and the values were positively correlated to the sperm concentration. Significant differences between the subfertile and the fertile stallions pairs in HIGH:LOW ratios were found in Pi and Cl concentrations. The highest concentrations of Ca and Mg were found in the last fractions with low sperm concentrations, with no significant differences between the fertile and the subfertile stallion pairs. The concentrations of K, Na and Cl were similar in HIGH and LOW fractions and in whole ejaculate samples. Pre-sperm fluid contained the highest concentrations of Na and Cl. Some of the possible variation in storage tolerance between ejaculates and ejaculatory fractions could perhaps be explained by differences in the composition of SP.

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Section: Discussionmentioning

confidence: 99%

Alkaline and Acid Phosphatase, β‐Glucuronidase and Electrolyte Levels in Fractionated Stallion Ejaculates

Kareskoski

Reilas

Sankari

et al. 2010

Reprod Domestic Animals

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“…In humans, the epididymis is the main source of this enzyme and its activity has been used as a biological marker for obstructive azoospermia [31]. In the bull and rat this enzyme is highly present in the cauda epididymis and has been found associated with vesicles in the rat [32,33]. The role of this enzyme in reproduction is not known, but transient association with sperm has been reported, suggesting a role in the modification of sperm protein sugars [34].…”

Section: Beta Mannosidasementioning

confidence: 99%

Identification, Proteomic Profiling, and Origin of Ram Epididymal Fluid Exosome-Like Vesicles1

Gatti

Métayer

Belghazi

et al. 2005

Biology of Reproduction

159

108

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Small membranous vesicles, between 25- and 75-nm diameter, were collected by high-speed centrifugation from the ram cauda epididymal fluid and were found to be normal constituents of this fluid and of the seminal plasma. The SDS-PAGE protein pattern of these vesicles was specific and very different from that of the caudal fluid, seminal plasma, sperm extract, and cytoplasmic droplets. After two-dimensional electrophoresis separation and mass spectrometry analysis, several proteins were identified and grouped into i) membrane-linked enzymes, such as dipeptidyl peptidase IV (DPP-IV), neprilysin (NEP), phosphodiesterase-I (E-NPP3), and protein G-beta; ii) vesicle-associated proteins, such as lactadherin (MFEG8-PAS6/7) and vacuolar ATPase; iii) several cytoskeleton-associated proteins, such as actin, ezrin and annexin; and iv) metabolic enzymes. The presence of some of these proteins as well as several different hydrophobic proteins secreted by the epididymis was further confirmed by immunoblotting. These markers showed that the majority of the vesicles originated from the cauda epididymal region. The physical and biochemical characteristics of these vesicles suggest they are the equivalent of the exosomes secreted by several cell types and epithelium. The main membrane-linked proteins of the vesicles were not retrieved in the extract from cauda or ejaculated sperm, suggesting that these vesicles did not fuse with sperm in vivo.

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Responses of seminal vesicle and testicular β‐glucuronidase and β‐N‐acetylglucosaminidase to testosterone and some metabolites in Heteropneustes fossilis (Bloch)

Chowdhury

Joy

2001

J. Exp. Zool.

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Intraperitoneal administration of testosterone for 20 days produced differential effects on beta-glucuronidase and beta-N-acetylglucosaminidase (beta-Glc) activity in seminal vesicle (SV) and testis of the catfish Heteropneustes fossilis in preparatory phase (March). The lower dosages of 0.25 and 0.5 microg/g body weight (BW) of the steroid did not alter enzyme activity, and the higher dosages (1.0 and 2.0 microg/g BW) inhibited it significantly. Under in vitro conditions, addition of ascorbate and fructose (0.5-100 mM) to the incubation medium influenced enzyme activity differentially. At concentrations 0.5 and 1.0 mM, both fructose and ascorbate were ineffective except for the inhibition of testicular beta-Glc activity in the 1.0 mM ascorbate group. At higher concentrations (10, 50, and 100 mM), ascorbate inhibited enzyme activity in a concentration-dependent manner. At 10 mM concentration of fructose, only testicular beta-Glc activity was inhibited, but at higher concentrations (50 and 100 mM), activities of both enzymes decreased uniformly in a concentration-dependent manner. The addition of glucose had no significant effect on the enzyme activity at any of the concentrations tested. The results suggest that the inhibitory effect of testosterone on enzyme activity may be mediated through androgen-dependent metabolites, such as fructose and ascorbate.

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ß-Glucuronidase in the Bull Seminal Plasma and Reproductive Organs

Cited by 6 publications

References 12 publications

Alkaline and Acid Phosphatase, β‐Glucuronidase and Electrolyte Levels in Fractionated Stallion Ejaculates

Alkaline and Acid Phosphatase, β‐Glucuronidase and Electrolyte Levels in Fractionated Stallion Ejaculates

Identification, Proteomic Profiling, and Origin of Ram Epididymal Fluid Exosome-Like Vesicles1

Responses of seminal vesicle and testicular β‐glucuronidase and β‐N‐acetylglucosaminidase to testosterone and some metabolites in Heteropneustes fossilis (Bloch)

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