Acid and bile tolerance of Lactobacillus isolated from chicken intestine

Abstract

L .Z . J I N, Y. W . H O, N . A BD U LL AH A ND S. J AL AL U DI N. 1998. Twelve Lactobacillus strains isolated from chicken intestine were used to investigate acid and bile tolerance in vitro. Ten out of the 12 strains were slightly affected by 0·3% bile salts, showing a delay of growth (d) of 0·6-37·2 min compared with growth in control cultures. Two strains were not affected by the bile salts. Of the 12 strains, seven could be arbitrarily classified as resistant (d ³ 15 min) and five as tolerant (15 min ³d ¾ 40 min). Lactobacillus strains from the caecum showed better tolerance to acid than those from the ileum. Generally, the survival of the ileal strains was very low at pH 1·0 and 2·0, and moderate at pH 3·0. In contrast, caecal Lactobacillus strains could survive at pH 1·0 for up to 2 h of incubation; growth was moderate at pH 2·0 and good at pH 3·0 and 4·0.

Effect of dietary supplementation of Lactobacilli and Streptococci cultures on the performance of broiler chickens

“…Non-significant effects in feed intake have previously been reported when broiler were given yoghurt (Sultan et al, 2006;Khan et al, 2011), Lactobacillus acidophilus (Salarmoini and Fooladi, 2011) and Lactobacillus johnsonii (Olnood et al, 2015). This result could be attributed to the inability of supplemented microorganisms to effect on the gut of the birds (Jin et al, 1998).…”

“…Improvements in growth performance, carcass quality, and feed efficiency in broiler chickens have been reported after supplementing yoghurt and lactobacilli probiotics with drinking water (Boostani et al, 2013). However, failure of probiotic cultures to colonize in the gut of the birds will be ineffective of their supplementation to enhance the performance of chicken (Jin et al, 1998;Salarmoini and Fooladi, 2011).…”

Survival of Probiotic Lactobacilli in Acidic Environments Is Enhanced in the Presence of Metabolizable Sugars

“…Lactobacillus species are considered intrinsically resistant to acid (51). Although there are differences between species and strains, organisms generally exhibit increased sensitivity at pH values below 3.0 (34,44). Hence, acid tolerance is accepted as one of the desirable properties used to select potentially probiotic strains.…”

Quorum Quenching Potentials of Probiotic Enterococcus Durans Lab38 Against Methicillin Resistant Staphylococcus Aureus

“…Tolerance to bile salts is an inevitable credential for probiotics, as bile salts reduce bacterial survival in the intestine by impairing its cell membrane [29]. Viable colony of LAB38 was found about 4.5 × 10 3 CFU/ml in treated and 5.5 × 10 3 CFU/ml in the control media.…”