Quorum Quenching Potentials of Probiotic Enterococcus Durans Lab38 Against Methicillin Resistant Staphylococcus Aureus

Sivakumar, N.; Boopathi, Seenivasan; Selvakumar, Gopal

doi:10.22159/ajpcr.2017.v10i4.17039

Cited by 11 publications

(3 citation statements)

References 39 publications

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“…It has also been shown that probiotic bacteria such as Enterococcus durans and Bacillus spp. inactivate the signal molecules of pathogenic bacteria by enzymatic action [20,21].…”

Section: Introductionmentioning

confidence: 99%

Quorum quenching potential of Enterococcus faecium QQ12 isolated from gastrointestinal tract of Oreochromis niloticus and its application as a probiotic for the control of Aeromonas hydrophila infection in goldfish Carassius auratus (Linnaeus 1758)

Vadassery

Pillai

2020

Braz J Microbiol

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Quorum quenching (QQ), the obstruction of quorum sensing, is the most attractive way to break down the N-acyl-homoserine lactones (AHL) molecules. This work was focused at isolating AHL degrading bacteria from gastrointestinal tract of Oreochromis niloticus, with abilities appropriate for use as probiotic in aquaculture. The presence of an autoinducer inactivation (aiiA) homolog gene and AHL inactivation assay showed that Enterococcus faecium QQ12, which was one among the 20 isolates, could rapidly degrade synthetic C6-HSL in vitro and hampered violacein production by Chromobacterium violaceum. It had excellent biodegrading ability of natural N-AHL produced by Aeromonas hydrophila, suggesting that it can be used as a potential quencher bacterium for disrupting the virulence of A. hydrophila. It was susceptible to all the five antibiotics tried out. The isolate grew well at pH 3.0-7.0, was resistant to high level of bile salts (0-0.9%) and 0.5% of phenol. QQ12 also exhibited high degree of auto-aggregation and co-aggregation, confirming that it possessed good probiotic attributes. Goldfish fed diet incorporated with 10 8 and 10 10 CFU g −1 of the QQ12 for 30 days showed 76.66-86.66% survival when challenged with A. hydrophila. The study indicates that Enterococcus faecium QQ12 could be used as a non-antibiotic feed additive in aquaculture to control bacterial diseases.

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“…It has also been shown that probiotic bacteria such as Enterococcus durans and Bacillus spp. inactivate the signal molecules of pathogenic bacteria by enzymatic action [20,21].…”

Section: Introductionmentioning

confidence: 99%

Quorum quenching potential of Enterococcus faecium QQ12 isolated from gastrointestinal tract of Oreochromis niloticus and its application as a probiotic for the control of Aeromonas hydrophila infection in goldfish Carassius auratus (Linnaeus 1758)

Vadassery

Pillai

2020

Braz J Microbiol

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“…Histological analysis of treated and untreated zebrafish was carried out as described by the previously established methods with minor modifications [28]. Zebrafish from the control and the treatment groups were taken and anaesthetized using 1% tricaine mesylate [29]. The anaesthetized zebrafish were fixed overnight in 1% formaldehyde and dehydrated using 80%, 90%, 95%, 100% ethanol, and 100% xylene.…”

Section: Histopathology Studiesmentioning

confidence: 99%

Ophthalmic Intervention of Naringenin Decreases Vascular Endothelial Growth Factor by Counteracting Oxidative Stress and Cellular Damage in In Vivo Zebrafish

Sudhakaran,

Chandran,

Sreekutty

et al. 2023

Molecules

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Diabetes Mellitus is a metabolic disease that leads to microvascular complications like Diabetic retinopathy (DR), a major cause of blindness worldwide. Current medications for DR are expensive and report multiple side effects; therefore, an alternative medication that alleviates the disease condition is required. An interventional approach targeting the vascular endothelial growth factor (VEGF) remains a treatment strategy for DR. Anti-VEGF medicines are being investigated as the main therapy for managing vision-threatening complications of DR, such as diabetic macular oedema. Therefore, this study investigated the effect of flavonoid naringenin (NG) from citrus fruits on inhibiting early DR in zebrafish. When exposed to 130 mM glucose, the zebrafish larvae developed a hyperglycaemic condition accompanied by oxidative stress, cellular damage, and lipid peroxidation. Similarly, when adult zebrafish were exposed to 4% Glucose, high glucose levels were observed in the ocular region and massive destruction in the retinal membrane. High glucose upregulated the expression of VEGF. In comparison, the co-exposure to NG inhibited oxidative stress and cellular damage and restored the glutathione levels in the ocular region of the zebrafish larvae. NG regressed the glucose levels and cellular damage along with an inhibition of macular degeneration in the retina of adult zebrafish and normalized the overexpression of VEGF as a promising strategy for treating DR. Therefore, intervention of NG could alleviate the domestication of alternative medicine in ophthalmic research.

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“…QS inhibition using QQ enzymes and analogue-based inhibitors was considered a promising alternative strategy over antibiotics. Employing bacteria that exert both QS inhibitory activity and probiotic effect 135,136 could be the most effective way for controlling aquatic pathogens while promoting the growth of aquatic animals. Recent research suggests that QQ enzymes have numerous advantages over analogue-based inhibitors.…”

Section: Perspectivementioning

confidence: 99%

Quorum sensing signals: Aquaculture risk factor

Priya

Boopathi

Murugan

et al. 2022

Reviews in Aquaculture

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Bacteria produce several virulence factors and cause massive mortality in fish and crustaceans. Abundant quorum sensing (QS) signals and high cell density are essentially required for the production of such virulence factors. Although several strategies have been developed to control aquatic pathogens through antibiotics and QS inhibition, the impact of pre‐existing QS signals in the aquatic environment has been overlooked. QS signals cause detrimental effects on mammalian cells and induce cell death by interfering with multiple cellular pathways. Moreover, QS signals not only function as a messenger, but also annihilate the functions of the host immune system which implies that QS signals should be designated as a major virulence factor. Despite QS signals' role has been well documented in mammalian cells, their impact on aquatic organisms is still at the budding stage. However, many aquatic organisms produce enzymes that degrade and detoxify such QS signals. In addition, physical and chemical factors also determine the stability of the QS signals in the aqueous environment. The balance between QS signals and existing QS signals degrading factors essentially determines the disease progression in aquatic organisms. In this review, we highlight the impact of QS signals on aquatic organisms and further discussed potential alternative strategies to control disease progression.

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Quorum Quenching Potentials of Probiotic Enterococcus Durans Lab38 Against Methicillin Resistant Staphylococcus Aureus

Cited by 11 publications

References 39 publications

Quorum quenching potential of Enterococcus faecium QQ12 isolated from gastrointestinal tract of Oreochromis niloticus and its application as a probiotic for the control of Aeromonas hydrophila infection in goldfish Carassius auratus (Linnaeus 1758)

Quorum quenching potential of Enterococcus faecium QQ12 isolated from gastrointestinal tract of Oreochromis niloticus and its application as a probiotic for the control of Aeromonas hydrophila infection in goldfish Carassius auratus (Linnaeus 1758)

Ophthalmic Intervention of Naringenin Decreases Vascular Endothelial Growth Factor by Counteracting Oxidative Stress and Cellular Damage in In Vivo Zebrafish

Quorum sensing signals: Aquaculture risk factor

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