Achromobacter sp. and Virgibacillus pantothenticus as models of thermo-tolerant lipase-producing marine bacteria from North Delta sediments (Egypt)

Abd-Elnaby, Hanan; Beltagy, Ehab A.; Abo-Elela, Gehan M.; El‐Sersy, Nermeen A.

doi:10.5897/ajmr2014.6828

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…Cotton seed oil is a cheap, commercial, and available economic source for lipase production. Therefore, it was previously used as a carbon source for lipase production (Abd-Elnaby et al, 2015). The current study indicated that the use of different lipids caused variations in lipase activity.…”

Section: Resultsmentioning

confidence: 99%

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

2018

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Lipases are enzymes that have the potential to hydrolyze triacylglycerol to free fatty acids and glycerol and have various applications. The aim of the present study was to isolate and screen marine bacteria for lipase production, optimize the production, and treat wastewater. A total of 20 marine bacterial isolates were obtained from the Mediterranean Sea and were screened for lipase production. All isolates were found to have lipolytic ability. The differences between the isolates were studied using RAPD-PCR. The most promising lipase producer (isolate 3) that exhibited the highest lipolytic hydrolysis (20 mm) was identified as Bacillus cereus HSS using 16S rDNA analysis and had the accession number MF581790. Optimization of lipase production was carried out using the Plackett–Burman experimental design with cotton seed oil as the inducer under shaking conditions at 10°C. The most significant factors that affected lipase production were FeSO4, KCl, and oil concentrations. By using the optimized culture conditions, the lipase activity increased by 1.8-fold compared with basal conditions. Immobilization by adsorption of cells on sponge and recycling raised lipase activity by 2.8-fold compared with free cells. The repeated reuse of the immobilized B. cereus HSS maintained reasonable lipase activity. A trial for the economic treatment of oily wastewater was carried out. Removal efficiencies of biological oxygen demand, total suspended solids, and oil and grease were 87.63, 90, and 94.7%, respectively, which is promising for future applications.

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Section: Resultsmentioning

confidence: 99%

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

2018

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Biochemical and molecular identification of lipolytic bacteria isolated from beverage industrial wastewater and optimization of lipase-secreting bacteria

Abinash

Khandayataray

Murthy

et al. 2021

Biomass Conv. Bioref.

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Medium Optimization for Protease Production via Moderately Halotolerant Virgibacillus Pantothenticus Using Response Surface Methodology

Banger

Nikerel

2020

Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji

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Virgibacillus pantothenticus is an industrially promising, yet scarcely studied, moderately halotolerant microorganism (up to 10% NaCl) with high activity protease production potential. Following Response Surface Methodology, we employed a Central Composite Design for the experiments and constructed a second order polynomial model to represent the resulting data. For medium optimization for protease production we optimized the resulting model. 32 experiments (following the central composite design scheme) where, carbon (glucose), nitrogen (ammonium sulfate), potassium (potassium phosphate monobasic) and magnesium (magnesium sulfate) sources were studied in the media. Bacterial growth, residual glucose and protease activities were determined at the 48 th hour of each experiment. The model was optimized and the concentrations were found for each parameter. Under the optimum conditions, the predicted protease activity is also experimentally verified and the model prediction was in very good agreement with experimental results. The interactions between medium components and their effect on cell growth and protease production are also sought. This work reports the improvements on protease production of a potentially interesting industrial host, Virgibacillus pantothenticus.

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Achromobacter sp. and Virgibacillus pantothenticus as models of thermo-tolerant lipase-producing marine bacteria from North Delta sediments (Egypt)

Abstract: Achrom mode ba Hana National Sediments used as a g North Delta from 9.8x10 Delta sedim selected th 4% (w/v) a identified a analysis. Th and NaCl respectively showed no only by V. p 014 and V. both strains 89.982 mmo tolerance to

Cited by 4 publications

References 29 publications

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

Biochemical and molecular identification of lipolytic bacteria isolated from beverage industrial wastewater and optimization of lipase-secreting bacteria

Medium Optimization for Protease Production via Moderately Halotolerant Virgibacillus Pantothenticus Using Response Surface Methodology

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