Acetylsalicylic acid (Aspirin®) and liver regeneration: experimental study in rats

Abstract

Objective: to evaluate the influence of acetylsalicylic acid (ASA) on cell proliferation after partial hepatectomy in rats. Methods: 40 male Wistar rats were separated into four groups of ten rats each. Groups 1 and 2 (controls): undergoing 30% partial hepatectomy and, after one day (group 1) and seven days (group 2), to euthanasia; daily administration of 0.9% saline solution (1mL per 200g of body weight). Groups 3 and 4 (experimental): undergoing 30% partial hepatectomy and, after one day (group 3) and seven days (group 4), to euthanasia; daily administration of ASA (40mg/mL, 1mL per 200g of body weight). The absolute number of cells stained with PCNA was counted in photomicrographs, in five fields, and it was calculated the mean of positive cells per animal and per group. Results: the final mean of PCNA+ cells per group was: in group 1, 17.57 ± 6.77; in group 2, 19.31 ± 5.30; in group 3, 27.46 ± 11.55; and, in group 4, 12.40 ± 5.23. There was no significant difference at the two evaluation times in the control group (p=0.491), but there was in the experimental group (p=0.020), with a lower number of PCNA+ cells on the seventh day. The comparison between the two groups, on the first day, showed more PCNA+ cells in the livers of the animals that received ASA (p=0.047), and on the seventh day the number was lower in the experimental group (p=0.007). Conclusion: ASA induced greater hepatocyte proliferation.

Anti-Inflammatory, Antioxidant, and Skin Regenerative Potential of Secondary Metabolites from Plants of the Brassicaceae Family: A Systematic Review of In Vitro and In Vivo Preclinical Evidence (Biological Activities Brassicaceae Skin Diseases)

“…The flavonoids present in Brassicaceae (such as quercetin and catechins) act by modulating the cells involved in inflammation, inhibiting the production of TNF-α and IL-1, modulating the activity of enzymes such as COX2, and modulating the nitric oxide-forming enzyme [ 61 , 62 ]. Their main mechanism of action is the inhibition of COX, which converts arachidonic acid into prostaglandins [ 63 ]. Another important class of phenolic compounds found in Brassicas are tannins, and their mechanisms of action are related to three properties: complexation with metal ions (such as iron, copper, and calcium), antioxidant and free radical scavenger activity, and the ability to compete with macromolecules, such as proteins and polysaccharides [ 64 , 65 ].…”

Potencial anti-inflamatório, antioxidante e regenerativo de metabólitos secundários de Brassicaceae na pele: uma revisão sistemática

“…The flavonoids present in Brassicaceae (such as quercetin and catechins) act by modulating the cells involved in inflammation, inhibiting the production of TNF-α and IL-1, modulating the activity of enzymes such as COX2, and modulating the nitric oxide-forming enzyme [61,62]. Their main mechanism of action is the inhibition of COX, which converts arachidonic acid into prostaglandins [63]. Another important class of phenolic compounds found in Brassicas are tannins, and their mechanisms of action are related to three properties: complexation with metal ions (such as iron, copper, and calcium), antioxidant and free radical scavenger activity, and the ability to compete with macromolecules, such as proteins and polysaccharides [64,65].…”