Acetylcholinesterase in the Erythrocyte Membrane

Igisu, Hideki; Matsumura, Hirohiko; Matsuoka, Masato

doi:10.7888/juoeh.16.253

Cited by 8 publications

(8 citation statements)

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“…Loss of membrane integrity is monitored by the inhibition of Na⁺, K⁺-ATPase, which maintains Na⁺ and K⁺ gradient across plasma membrane that is important for cell function [ 49 ]. Studies have shown a correlation between acetylcholinesterase inhibition in blood and damage in target tissues [ 50 ]. Na⁺, K⁺-ATPase and acetylcholinesterase activities declined in erythrocytes in presence of H 2 O 2 but Cr pre-treatment greatly restored them towards control levels.…”

Section: Discussionmentioning

confidence: 99%

Diminution of Oxidative Damage to Human Erythrocytes and Lymphocytes by Creatine: Possible Role of Creatine in Blood

Qasim

Mahmood

2015

PLoS ONE

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Creatine (Cr) is naturally produced in the body and stored in muscles where it is involved in energy generation. It is widely used, especially by athletes, as a staple supplement for improving physical performance. Recent reports have shown that Cr displays antioxidant activity which could explain its beneficial cellular effects. We have evaluated the ability of Cr to protect human erythrocytes and lymphocytes against oxidative damage. Erythrocytes were challenged with model oxidants, 2, 2'-azobis(2-amidinopropane) dihydrochloride (AAPH) and hydrogen peroxide (H2O2) in the presence and absence of Cr. Incubation of erythrocytes with oxidant alone increased hemolysis, methemoglobin levels, lipid peroxidation and protein carbonyl content. This was accompanied by decrease in glutathione levels. Antioxidant enzymes and antioxidant power of the cell were compromised while the activity of membrane bound enzyme was lowered. This suggests induction of oxidative stress in erythrocytes by AAPH and H2O2. However, Cr protected the erythrocytes by ameliorating the AAPH and H2O2 induced changes in these parameters. This protective effect was confirmed by electron microscopic analysis which showed that oxidant-induced cell damage was attenuated by Cr. No cellular alterations were induced by Cr alone even at 20 mM, the highest concentration used. Creatinine, a by-product of Cr metabolism, was also shown to exert protective effects, although it was slightly less effective than Cr. Human lymphocytes were similarly treated with H2O2 in absence and presence of different concentrations of Cr. Lymphocytes incubated with oxidant alone had alterations in various biochemical and antioxidant parameters including decrease in cell viability and induction of DNA damage. The presence of Cr attenuated all these H2O2-induced changes in lymphocytes. Thus, Cr can function as a blood antioxidant, protecting cells from oxidative damage, genotoxicity and can potentially increase their lifespan.

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Section: Discussionmentioning

confidence: 99%

Diminution of Oxidative Damage to Human Erythrocytes and Lymphocytes by Creatine: Possible Role of Creatine in Blood

Qasim

Mahmood

2015

PLoS ONE

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“…Erythrocyte AChE plays an important role in the preservation of the integrity of the red cell. Markedly reduced erythrocyte AChE activity was demonstrated in cases of paroxysmal nocturnal haemoglobinuria (PNH) (Auditore & Hartmann, 2004).Previous studies showed a correlation between AChE inhibition in blood and inhibition in target tissues (Kale et al , 1999, Igisu et al , 1994). Clomazone significantly inhibited AChE activity in brain and muscle of silver catfish (Rhamdia quelen) exposed to 5, 10 and 20 mg/L for 96 h (dos Santos Miron et al , 2005).…”

Section: Discussionmentioning

confidence: 99%

“…AChE has an essential role in acetylcholine-mediated neurotransmission. It is present in the cholinergic synapses in the central nervous system as well as in neuromuscular synapses where it rapidly hydrolyzes acetylcholine (Igisu et al , 1994; Jha & Rizvi, 2009). The determination of AChE activity in blood is a great consideration in the diagnosis of poisonings caused by reversible and irreversible inhibitors of this enzyme including pesticides (Bukowska & Hutnika, 2006).…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress biomarkers and acetylcholinesterase activity in human erythrocytes exposed to clomazone (in vitro)

Santi¹,

Menezes²,

Duarte³

et al. 2011

Interdisciplinary Toxicology

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The aim of this study was to investigate the effect of clomazone herbicide on oxidative stress biomarkers and acetylcholinesterase activity in human erythrocytes in in vitro conditions. The activity of catalase (CAT), superoxide dismutase (SOD) and acetylcholinesterase (AChE), as well as the levels of thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) were measured in human erythrocytes exposed (in vitro) to clomazone at varying concentrations in the range of 0, 100, 250 and 500 µg/L for 1 h at 37 °C.TBARS levels were significantly higher in erythrocytes incubated with clomazone at 100, 250 and 500 µg/L. However, erythrocyte CAT and AChE activities were decreased at all concentrations tested. SOD activity was increased only at 100 µg/L of clomazone. GSH levels did not change with clomazone exposure. These results clearly showed clomazone to induce oxidative stress and AChE inhibition in human erythrocytes (in vitro). We, thus, suggest a possible role of ROS on toxicity mechanism induced by clomazone in humans.

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“…AChE is present in central nervous system and skeletal muscle and exists in the erythrocyte membranes, whereas BChE is secreted into plasma and synthesized in the liver. Erythrocytes AChE is located at the outside of the erythrocytes membrane [ 14 ] where it constitutes the Yt blood group antigen [ 15 ]. The physiological role of AChE is not known but has the same function as the enzyme involved in synaptic transmission, and its measurement is used to mirror the effects on the nervous system [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Changes in Cholinesterase Activity in Blood of Adolescent with Metabolic Syndrome after Supplementation with Extract fromAronia melanocarpa

Duchnowicz

Ziobro

Rapacka

et al. 2018

BioMed Research International

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Obesity and metabolic syndrome (MetS) are growing problems among children and adolescents. There are no reports of changes in the activity of butyrylcholinesterase (BChE) in children and adolescents with metabolic syndrome especially after supplementation with extract from Aronia melanocarpa. Materials studied included plasma and erythrocytes isolated from peripheral blood of patients with MetS and healthy subjects. We have estimated the following parameters: acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activity, lipid peroxidation and lipids levels in plasma, and erythrocytes membrane. In patients with MetS, a significant increase in AChE and BChE activity, higher LDL-cholesterol and triacylglycerol levels, and lower HDL-cholesterol level were observed. Supplementation with A. melanocarpa extract resulted in mild but statistically significant reduction of total cholesterol, LDL-cholesterol, and triacylglycerol levels and caused an increase in HDL-cholesterol level and a decrease in lipid peroxidation in plasma patients with MetS. Additionally, a decrease in lipid peroxidation and cholesterol level and a decrease in AChE activity in the erythrocyte membranes after supplementation with A. melanocarpa were noted. Summarizing, an increase in AChE and BChE activity and disruption of lipid metabolism in patients with MetS were observed. After supplementation of MetS patients with A. melanocarpa extract, a decrease in AChE activity and oxidative stress was noted.

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Acetylcholinesterase in the Erythrocyte Membrane

Cited by 8 publications

References 0 publications

Diminution of Oxidative Damage to Human Erythrocytes and Lymphocytes by Creatine: Possible Role of Creatine in Blood

Diminution of Oxidative Damage to Human Erythrocytes and Lymphocytes by Creatine: Possible Role of Creatine in Blood

Oxidative stress biomarkers and acetylcholinesterase activity in human erythrocytes exposed to clomazone (in vitro)

Changes in Cholinesterase Activity in Blood of Adolescent with Metabolic Syndrome after Supplementation with Extract fromAronia melanocarpa

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