Diminution of Oxidative Damage to Human Erythrocytes and Lymphocytes by Creatine: Possible Role of Creatine in Blood

Qasim, Neha; Mahmood, Riaz

doi:10.1371/journal.pone.0141975

Cited by 47 publications

(32 citation statements)

References 70 publications

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“…The level of MDA, which is generated as an end product during the oxidation of lipids, was used as a marker of lipid peroxidation (Qasim and Mahmood 2015). Treatment of rats with furan increased lipid peroxidation as shown by elevated MDA levels.…”

Section: Discussionmentioning

confidence: 99%

Lycopene Protects the Diabetic Rat Kidney Against Oxidative Stress-mediated Oxidative Damage Induced by Furan

Pandır

Ünal

Baş

2016

Braz. arch. biol. technol.

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Furan is a food and environmental contaminant and a potent carcinogen in animals. Lycopene is one dietary carotenoid found in fruits such as tomato, watermelon and grapefruit. The present study was designed to explore the protective effect of lycopene against furan-induced oxidative damage in streptozotocin (STZ)-induced diabetic rat kidney. At the end of the experimental period (28 days), we found that lycopene markedly decreased the malondialdehide (MDA) levels in the kidney, urea, uric acid and creatinine levels in the serum of furan-treated rats. The increase of histopathology in the kidney of furan-treated rats were effectively suppressed by lycopene. Furthermore, lycopene markedly restored superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities in the kidney of furan-treated rats.In conclusion, these results suggested that lycopene could protect the rat kidney against furan-induced injury by improving renal function, attenuating histopathologic changes, reducing MDA production and renewing the activities of antioxidant enzymes.

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Section: Discussionmentioning

confidence: 99%

Lycopene Protects the Diabetic Rat Kidney Against Oxidative Stress-mediated Oxidative Damage Induced by Furan

Pandır

Ünal

Baş

2016

Braz. arch. biol. technol.

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“…1 Cr is naturally produced and stored in muscles where it is involved in energy generation. It is widely used, especially by athletes, 3 as a staple supplement to improve physical performance. Recent reports showed that Cr displays antioxidant activity which could explain its beneficial cellular effects.…”

Section: Introductionmentioning

confidence: 99%

“…Some authors. 3 (Figure 2), have evaluated the ability of Cr to protect human erythrocytes and lymphocytes against oxidative damage. Erythrocytes were challenged with model oxidants, 2, 2'-azobis (2-amidinopropane) dihydrochloride (AAPH) and hydrogen peroxide (H 2 O 2 ) both in the presence and absence of Cr.…”

Section: Introductionmentioning

confidence: 99%

“…Antioxidant enzymes and antioxidant power of the cell were compromised while the activity of membrane bound scavenger enzymes were lowered. 3 In a recent paper 5 authors demonstrated that hypertriglyceridemia affects Red Blood Cell membrane oxidative status (increase of [MDA] and [HNE] to membrane protein ratio) is increased by high levels of blood cholesterol, but selectively by hypertriglyceridemia. 5,6 It is known that Cr protects the erythrocytes by attenuating the AAPH and H 2 O 2 induced alterations.…”

Section: Introductionmentioning

confidence: 99%

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Do Creatinine Blood Levels Act upon Fatty Acid Metabolites in Human Red Blood Cell Membranes?

Gallo

Martino

2020

FRA

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Objectives: The aim of the present research is to correlate creatininemia and uricemia to HNE and MDA levels of Red Blood Cell purified membranes in comparison to those of normal subjects to characterize the effects of oxidative stress. Methods: The studied population consisted of 10 patients with endogenous both hypercreatininemic hyperuricemic and ten normal subjects. The measurement of total proteins in RBC ghosts and HNE and MDA are conducted on blood samples of patients. Results: The obtained data evidence that both creatinine and uric acid levels have influence on the ratio of both MDA/protein and HNE/protein content on RBC ghosts, demonstrating their possible protective role against oxidative stress at low concentrations in blood and oxidizing power at higher concentrations. Conclusion: The displayed results in this study suggest that at low concentrations of creatinine and uric acid there is oxidative stress in human erythrocyte membrane, but if creatinine and uric acid have slowed down to sufficient limits there is also oxidative stress, as demonstrated by MDA and HNE levels; only if the concentration of these two substances overwhelms a threshold. .) Studies on the mitochondrial metabolism by either environmental or endogenous regulating factors, the regulation of interstitial compartment, bone and the bonelike biomaterials structure and properties. From 1992 he refined fluorescence microscopy and image analysis methods to evaluate ion transport, NO metabolism and oxidative stress of cells in culture . From 2006 he studies also oxidative stress and enzyme scavenging activities in experimental animals and human subjects. He has 60 international publications with referees.

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“…mitochondria), the pentose phosphate pathway becomes its only way to produce NADPH [5]. As a result, compared with normal nucleus cells, erythrocytes seem to be more susceptible to oxidative stress , and eryptosis, the molecular programming process characterized by cell suicide, could be more readily induced [6].…”

Section: Introductionmentioning

confidence: 99%

Vitamin C Inhibits Aggravated Eryptosis by Hydrogen Peroxide in Glucose-6-Phosphated Dehydrogenase Deficiency

Shan

Yang

et al. 2016

Cell Physiol Biochem

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Background/Aims: The study was aimed to investigate if vitamin C could exert protective effects on development of eryptosis caused by glucose-6-phosphate dehydrogenase (G6PD) deficiency and hydrogen peroxide. Methods: Isolated erythrocytes with different G6PD activity (normal or deficient) were divided into various groups treated by either Vitamin C or H₂O₂. Phosphatidylserine (PS) extroversion rate was detected by Annexin V binding. The intracellular Ca²⁺ concentration was detected by Fluo3-fluorescence, and western blot was used to detect the expression of apoptosis factor caspase 3. Results: Compared with the blank group, the PS extroversion rate (P < 0.001), intracellular Ca²⁺ concentration (P < 0.001) and active caspase 3 expression level (P < 0.05) of erythrocytes significantly increased after treatment of 0.05% H₂O₂. Then the index of eryptosis significantly decreased after erythrocytes were treated with Vitamin C (1 mg/ml) for 30 min (all P < 0.05). The decline in erythrocytes with G6PD normal activity was more significant than those with G6PD deficiency. Conclusion: Vitamin C could effectively inhibit the eryptosis contributed by H₂O₂ oxidative stress, and the suppression on eryptosis with G6PD normal activity was more effective than that with G6PD deficiency.

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Diminution of Oxidative Damage to Human Erythrocytes and Lymphocytes by Creatine: Possible Role of Creatine in Blood

Cited by 47 publications

References 70 publications

Lycopene Protects the Diabetic Rat Kidney Against Oxidative Stress-mediated Oxidative Damage Induced by Furan

Lycopene Protects the Diabetic Rat Kidney Against Oxidative Stress-mediated Oxidative Damage Induced by Furan

Do Creatinine Blood Levels Act upon Fatty Acid Metabolites in Human Red Blood Cell Membranes?

Vitamin C Inhibits Aggravated Eryptosis by Hydrogen Peroxide in Glucose-6-Phosphated Dehydrogenase Deficiency

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