Acetylcholinesterase Biosensor Based On Mesoporous Hollow Carbon Spheres/Core-Shell Magnetic Nanoparticles-Modified Electrode for the Detection of Organophosphorus Pesticides

Luo, Ruiping; Feng, Zijie; Shen, Guannan; Xiu, Yi; Zhou, Yukun; Niu, Xiaodi; Wang, Hongsu

doi:10.3390/s18124429

Cited by 28 publications

(18 citation statements)

References 48 publications

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“…The higher weight of the multi-shelled hollow nanostructures can cause to increase the energy density 6,7 . They are designed with a hybrid of internal architectures such as core(s)-in-hollow-shell(s), multishell and multi-chambers 8,9 . P19 protein is a suppressing factor in the silencing pathway as a common form of antiviral defense.…”

mentioning

confidence: 99%

Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Ghazizadeh

Moosavifard

Daneshmand

et al. 2020

Sci Rep

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electrochemical sensors are the tools to detect the accurate and sensitive miRs. there is the challenge to increase the power and sensitivity of the surface for the electrochemical sensor. We design a viruslike hallow structure of cuco 2 o 4 that it holds the large amounts of p19 protein by mimicking of inherent virus (Carnation italian ringspot virus) to detect 21mir with the limit of detection (LOD = 1aM). The electrochemical measurements are performed between the potentials at −0.3 V and +0.3 V with 1 mM [fe(cn) 6 ] −3/−4. After dropping the cuco 2 o 4 on the Scpe (screen carbon printed electrode), the sensor is turned on due to the high electrochemical properties. Then, p19 proteins move into the hallow structure and inhibit the exchange of electrochemical reactions between the shells and the sensor is turned off. Then, adding the duplexes of RNA/miRs cause to increase the electrochemical property of p19 due to the change of p19 conformation and the system is turned on, again. So, for the first time, a virus-like hallow structure has been used to detect the 21miR in the human serum, MCF-7, Hella cells, with high sensitivity, specificity, and reproducibility in few minutes.

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mentioning

confidence: 99%

Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Ghazizadeh

Moosavifard

Daneshmand

et al. 2020

Sci Rep

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“…From a molecular perspective, AchE can promote the hydrolysis of indophenol acetate and the activity of enzymes is inhibited with the addition of pesticides, the principle is shown in Figure 3c. This reaction involves the transfer of electrons and changes in impedance (Luo et al, 2018). Hydrolysis reaction rate of acetylcholinesterase and indophenol acetate will change with time.…”

Section: Methodsmentioning

confidence: 99%

“…This reaction involves the transfer of electrons and changes in impedance (Luo et al, 2018). Hydrolysis reaction rate of acetylcholinesterase and indophenol acetate will change with time.…”

Section: Principle Of Identification By Impedance Time-sequence Spementioning

confidence: 99%

Pesticide residues identification by impedance time‐sequence spectrum of enzyme inhibition on multilayer paper‐based microfluidic chip

Yang

et al. 2020

J Food Process Engineering

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Different organophosphorus pesticides (OPs) normally vary in the level of toxicity to human health, but common electrochemical identification methods usually require complex electrode fabrication processes and high detection cost. To overcome these problems, a pesticide residues identification method based on multilayer paper-based microfluidic chip was proposed. The microfluidic chip integrates the enzyme inhibition and a label-free screen-printed carbon electrode (SPCE). Three different pesticides were selected as study objects, respectively, avermectin, phoxim, dimethoate. This chip realizes on-chip identification via analyzing impedance time-sequence spectrum data aroused by the enzyme inhibition during the 15 min period. A classification model based on principal component analysis (PCA) and support vector machines (SVMs) was established to evaluate these data. Furthermore, the real-world sample (lettuce) was tested to verify the feasibility of the proposed detection method. The results showed that the classification accuracy was achieved around 93% in multiple experiments. Therefore, the method has good stability and specificity in rapid identification of different pesticides. Practical Applications Identification of pesticide types is essential to human health, for this, impedance time-sequence spectrum data aroused by the enzyme inhibition during the 15 min period was used to establish a classification model to identity different types of pesticides. In this article, multilayer paper-based microfluidic chip and a label-free screenprinted carbon electrode (SPCE) was proposed without complicated operation steps and expensive cost, which provide a basis for establishment of the rapid pesticide discriminator.

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“…The electrochemical performance mainly depends on the active material, suitable structure, and pore size (Zhang et al., 2014). The results show that micropores can significantly increase the specific surface area of carbon materials, improve the contact site of charge in carbon materials, enhance the storage capacity of charge in supercapacitors, and improve the electrochemical performance (Luo et al., 2018). In this study, an Al‐based MOF was used as a precursor to prepare porous carbon.…”

Section: Introductionmentioning

confidence: 99%

Penicillin biosensor based on rhombus‐shaped porous carbon/hematoxylin/penicillinase

Wang

Xiu

et al. 2021

Journal of Food Science

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In this experiment, we designed an electrochemical sensor using penicillinase (Pen X)-rhombus porous carbon (RPC) as the detection element and hematoxylin as the indicator to detect low concentrations of penicillin sodium (Pen G). A differential pulse voltammetry (DPV) method was used to detect Pen G in the concentration range of 10 -8 -10 -5 mg⋅mL -1 under optimal experimental conditions. The results showed that the peak current value and the logarithm of Pen G concentration showed a good linear relationship (R 2 = 0.9915), and the LOD was 2.68 × 10 -7 mg⋅mL -1 (S/N = 3). The actual milk samples were detected by the addition method and compared with the high-performance liquid phase method; no significant difference was found in the detection results. The working electrode prepared by cross-linking method not only extends the service life of the sensor, but also improves the sensitivity and reproducibility of the sensor. It can also be used to detect the Pen G residue in the actual milk samples repeatedly. Practical Application: In this study, an electrochemical sensor for the rapid detection of penicillin sodium in milk was prepared, which has good sensitivity and fast detection speed.

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Acetylcholinesterase Biosensor Based On Mesoporous Hollow Carbon Spheres/Core-Shell Magnetic Nanoparticles-Modified Electrode for the Detection of Organophosphorus Pesticides

Cited by 28 publications

References 48 publications

Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Pesticide residues identification by impedance time‐sequence spectrum of enzyme inhibition on multilayer paper‐based microfluidic chip

Penicillin biosensor based on rhombus‐shaped porous carbon/hematoxylin/penicillinase

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