Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Ghazizadeh, Elham; Moosavifard, Seyyed Ebrahim; Daneshmand, Negin; Kaverlavani, Saeid Kamari

doi:10.1038/s41598-020-66393-z

Cited by 10 publications

(8 citation statements)

References 30 publications

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“…In the concentration range between 5.0 × 10 −13 M and 3.8 × 10 −8 M, the regression equation is ΔR/R = 6.2 × 10 −2 + 1.8 × 10 −2 Ln C, with a coefficient of determination R 2 = 0.9957 (N = 6). The detection limit (LOD) of MEL is calculated using the equation LOD = 3.3 SD/b, where (SD) and (b) are the standard deviation of the intercept and the slope of the calibration plot, respectively [ 42 ], and the calculated LOD is equal to 1.8 × 10 −12 M (at S/N = 3). Moreover, the reproducibility of the modified electrode GCE/PN–AuNPs–MB was assessed by the measurement of the response of three different modified electrodes, prepared with the same modification, to a 3 × 10 −12 M of MEL solution, by impedance spectroscopy.…”

Section: Resultsmentioning

confidence: 99%

Sensor Based on a Poly[2-(Dimethylamino)ethyl Methacrylate-Co-Styrene], Gold Nanoparticles, and Methylene Blue-Modified Glassy Carbon Electrode for Melamine Detection

Aberkane

Abdou

Zine

et al. 2021

Sensors

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Melamine has been used as a non-protein nitrogenous additive in food products to artificially increase the apparent “false” protein content. Melamine is known as a dangerous and poisonous substance for human health and it causes diverse diseases. An electrochemical sensor for melamine detection has been developed by modification of a glassy carbon electrode using copolymer poly[DMAEMA-co-styrene], gold nanoparticles, and methylene blue. The characterization of the modified electrode was conducted using several analysis techniques including cyclic voltammetry (CV), differential pulse voltammetry (DPV), chronoamperometry (CA), and electrochemical impedance spectroscopy (EIS). The electrochemical detection of melamine was performed by impedance spectroscopy. Obtained results revealed that the developed sensor has a large detection range from 5.0 × 10−13 to 3.8 × 10−8 M with a low detection limit of 1.8 × 10−12 M (at S/N = 3). Various interfering species such as phenol, hydroquinone, and bisphenol A have been used and their behavior on modified electrode has been studied.

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Section: Resultsmentioning

confidence: 99%

Sensor Based on a Poly[2-(Dimethylamino)ethyl Methacrylate-Co-Styrene], Gold Nanoparticles, and Methylene Blue-Modified Glassy Carbon Electrode for Melamine Detection

Aberkane

Abdou

Zine

et al. 2021

Sensors

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“…Adding the SARS-CoV-2 Antigen on the MV-gal1/SCPE-GNP shows that the peak currents increased from 13.9 to 15.4 µA and the ΔEp decreased from 83 to 73 mV, due to covering the glycosylation bond by gal1 and glycan of spike of SARS-CoV-2. A recent study of Kajiyazdi was showed that covering the glycosylation bonds when lectin used as a synthetic receptor to identify the glycan on the tumor cells of AML disease 26 , 27 . Our results indicate the possible connection of the gal1 with NTD-domain (Glycan domain) of the SARS-CoV-2 Antigen by increasing the current peak, which it causes to the reduction of electrochemical exchanges with the [Fe(CN) 6 ] −3/−4 redox probe, too.…”

Section: Resultsmentioning

confidence: 99%

Dual electrochemical sensing of spiked virus and SARS-CoV-2 using natural bed-receptor (MV-gal1)

Ghazizadeh

Neshasteh-Riz

Firoozabadi

et al. 2021

Sci Rep

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It has been necessary to use methods that can detect the specificity of a virus during virus screening. In this study, we use a dual platform to identify any spiked virus and specific SARS-CoV-2 antigen, sequentially. We introduce a natural bed-receptor surface as Microparticle Vesicle-Galactins1 (MV-gal1) with the ability of glycan binding to screen every spiked virus. MV are the native vesicles which may have the gal-1 receptor. Gal-1 is the one of lectin receptor which can bind to glycan. After dropping the MV-gal1 on the SCPE/GNP, the sensor is turned on due to the increased electrochemical exchange with [Fe(CN)6]−3/−4 probe. Dropping the viral particles of SARS-CoV-2 cause to turn off the sensor with covering the sugar bond (early screening). Then, with the addition of Au/Antibody-SARS-CoV-2 on the MV-gal1@SARS-CoV-2 Antigen, the sensor is turned on again due to the electrochemical amplifier of AuNP (specific detection).For the first time, our sensor has the capacity of screening of any spike virus, and the specific detection of COVID-19 (LOD: 4.57 × 102 copies/mL) by using the natural bed-receptor and a specific antibody in the point of care test.

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“…The bioreceptor-bioanalyte interaction may lead to the change in conductance of the electrolyte solution and can be leveraged as a biosensing signal for developing an impedimetric or conductometric biosensor [56]. The electrodes play a pivotal role in the performance of an electrochemical biosensor.…”

Section: Ace2-based Electrochemical Detection Of Sars-cov-2mentioning

confidence: 99%

Angiotensin-converting enzyme 2-based biosensing modalities and devices for coronavirus detection

Gul¹,

Zhai²,

Zhong³

et al. 2022

Preprint

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Rapid and cost-effective diagnostic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have become a critical and valuable weapon for the coronavirus disease 2019 (COVID-19) pandemic response. SARS-CoV-2 invasion is primarily mediated by human angiotensin-converting enzyme 2 (hACE2). Recent developments in ACE2-based SARS-CoV-2 detection modalities accentuate the potential of this natural host-virus interaction for developing point-of-care (POC) COVID-19 diagnostic tools and devices. Although research on harnessing ACE2 for SARS-CoV-2 detection is in its infancy, some interesting biosensing devices have been developed, showing the commercial viability of this intriguing new approach. The exquisite performance of the reported ACE2-based COVID-19 biosensors provides opportunities for researchers to develop rapid detection tools suitable for testing at points of entry, workplaces, or congregate scenarios in order to effectively implement pandemic control and management plans. However, to be considered as an emerging approach, the rationale for ACE2-based biosensing needs to be critically and comprehensively surveyed and discussed. Herein, we review the current status of ACE2-based detection methods, the signal transduction principles in ACE2 biosensors and the development trend in the future. We discuss the challenges to development of ACE2-biosensors and delineate prospects for their use, along with recommended solutions and suggestions

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Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Cited by 10 publications

References 30 publications

Sensor Based on a Poly[2-(Dimethylamino)ethyl Methacrylate-Co-Styrene], Gold Nanoparticles, and Methylene Blue-Modified Glassy Carbon Electrode for Melamine Detection

Sensor Based on a Poly[2-(Dimethylamino)ethyl Methacrylate-Co-Styrene], Gold Nanoparticles, and Methylene Blue-Modified Glassy Carbon Electrode for Melamine Detection

Dual electrochemical sensing of spiked virus and SARS-CoV-2 using natural bed-receptor (MV-gal1)

Angiotensin-converting enzyme 2-based biosensing modalities and devices for coronavirus detection

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