Acetylcholinesterase (AChE) and heat shock proteins (Hsp70) of gypsy moth (Lymantria dispar L.) larvae in response to long-term fluoranthene exposure

Mrdaković, Marija; Ilijin, Larisa; Vlahović, Milena; Matić, Dragana; Gavrilović, Anja; Mrkonja, Aleksandra; Perić-Mataruga, Vesna

doi:10.1016/j.chemosphere.2016.06.059

Cited by 34 publications

(14 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…HSPs are rapidly synthesized in response to various environmental stressors, including heat shock, cold shock, pesticide application, and heavy metal exposure (Huang and Kang, 2007;Rinehart et al, 2007;Shu et al, 2011;Sun et al, 2014;Yang et al, 2016). The expression of HSP70 is significantly induced in Drosophila melanogaster, Nilaparvata lugens, Lymantria dispar, and Apolygus lucorum to increase resistance against applied pesticides (Mrdaković et al, 2016;Sun et al, 2016;Lu et al, 2017). Thus, we speculate that DEGs encoding HSPs participate in the response of T. castaneum to A. vulgaris EO.…”

Section: Discussionmentioning

confidence: 86%

Insecticidal Activity of Artemisia vulgaris Essential Oil and Transcriptome Analysis of Tribolium castaneum in Response to Oil Exposure

et al. 2020

View full text Add to dashboard Cite

Red flour beetle (Tribolium castaneum) is one of the most destructive pests of stored cereals worldwide. The essential oil (EO) of Artemisia vulgaris (mugwort) is known to be a strong toxicant that inhibits the growth, development, and reproduction of T. castaneum. However, the molecular mechanisms underlying the toxic effects of A. vulgaris EO on T. castaneum remain unclear. Here, two detoxifying enzymes, carboxylesterase (CarEs) and cytochrome oxidase P450 (CYPs), were dramatically increased in red flour beetle larvae when they were exposed to A. vulgaris EO. Further, 758 genes were differentially expressed between EO treated and control samples. Based on Gene Ontology (GO) analysis, numerous differentially expressed genes (DEGs) were enriched for terms related to the regulation of biological processes, response to stimulus, and antigen processing and presentation. Our results indicated that A. vulgaris EO disturbed the antioxidant activity in larvae and partially inhibited serine protease (SP), cathepsin (CAT), and lipase signaling pathways, thus disrupting larval development and reproduction as well as down-regulating the stress response. Moreover, these DEGs showed that A. vulgaris indirectly affected the development and reproduction of beetles by inducing the expression of genes encoding copper-zinc-superoxide dismutase (CuZnSOD), heme peroxidase (HPX), antioxidant enzymes, and transcription factors. Moreover, the majority of DEGs were mapped to the drug metabolism pathway in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Notably, the following genes were detected: 6 odorant binding proteins (OBPs), 5 chemosensory proteins (CSPs), 14 CYPs, 3 esterases (ESTs), 5 glutathione S-transferases (GSTs), 6 UDP-glucuronosyltransferases (UGTs), and 2 multidrug resistance proteins (MRPs), of which 8 CYPs, 2 ESTs, 2 GSTs, and 3 UGTs were up-regulated dramatically after exposure to A. vulgaris EO. The residual DEGs were significantly down-regulated in EO exposed larvae, implying that partial compensation of metabolism detoxification existed in treated beetles. Furthermore, A. vulgaris EO induced overexpression of OBP/CYP,

show abstract

Section: Discussionmentioning

confidence: 86%

Insecticidal Activity of Artemisia vulgaris Essential Oil and Transcriptome Analysis of Tribolium castaneum in Response to Oil Exposure

et al. 2020

View full text Add to dashboard Cite

show abstract

“…A inibição de sua atividade pode induzir mudanças no sitio ativo da enzima, levando ao acúmulo de ACh dentro das sinapses interrupção do sinal de transmissão. 6 A principal toxicidade de alguns OFs é atribuída à sua capacidade de inibir a atividade da AChE, devido ao fato desta enzima estar presente também em mamíferos. Essa inibição impede a quebra da ACh nas sinapses colinérgicas, o que pode afetar severamente o sistema nervoso humano.…”

Section: Ofs E Inibição Da Enzima Acheunclassified

“…71 As mudanças na atividade de AChE foram usadas como biomarcador de neurotoxicidade e descritas em espécies de insetos como consequência de ações de inseticidas e biopesticidas. 6…”

Section: Ofs E Inibição Da Enzima Acheunclassified

“…A inibição da sua atividade por inseticidas OFs, podem induzir mudanças no sítio ativo da enzima, levando ao acúmulo de acetilcolina dentro da sinapses e interromper a transmissão do sinal, podendo causar a morte. 6 No entanto, a dinâmica da interação da AChE com os OFs depende da sua afinidade pela enzima, o qual é comumente representado como constante de afinidade Ks. 7 Em contrapartida, compostos inibidores da AChE têm sido amplamente reconhecidos como um tratamento eficaz para a doença de Alzheimer, pois atuam impedindo o colapso da acetilcolina (ACh), através da inibição da AChE nas regiões do cérebro (córtex e hipocampo) relevantes para doença de Alzheimer, atrasando assim o desenvolvimento de deficiências cognitiva e neurodegeneração e aliviando o sintomas.…”

Section: Introductionunclassified

See 1 more Smart Citation

Organophosphorus Compounds and their Role in Agriculture

et al. 2018

View full text Add to dashboard Cite

The agricultural need for greater food production in smaller and smaller spaces led to the search for technologies capable of supplying higher yield per area, which separates the use of agricultural pesticides. In addition, attack by microorganisms such as fungi, bacteria and viruses is one of the safest causes of post-harvest losses of fruits and vegetables. Among the products used as pesticides, organophosphorus compounds (OPs) are even more important for agricultural activities around the world, constituting a very diverse family of organic chemicals. OPs have been banned from agriculture in several countries because of their high toxicity, once they can be inhibitors of the enzyme acetylcholinesterase (AChE), which plays a critical role in the proper functioning of nerve cells. However, a toxicity is related to molecule structure. Thus, a new generation of OPs have studied, non-inhibitors of AChE and with greater biological activity.

show abstract

“…AChE, primarily spreads in the nervous tissue, swiftly interferes in the catalyzation of the hydrolysis of the neurotransmitter acetylcholine, and causes the cancellation of transmission of nerve impulse, thus ensures the physiological function of the body normally. For nervous system AChE is a significant member, so any negative effects on AChE efficiency may cause to neurotoxicity (Mrdaković et al, ). BChE related in many physiological factors, the most exclusive is the hydrolysis of both choline and noncholine esters, such as aspirin, cocaine, and succinylcholine; therefore, it has a significant role in neurotransmission and anesthesia.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical content, antioxidant activity, and enzyme inhibition effect ofSalvia eriophoraBoiss. & Kotschy against acetylcholinesterase, α-amylase, butyrylcholinesterase, and α-glycosidase enzymes

et al. 2019

View full text Add to dashboard Cite

Many taxa of Salvia genus have been used in herbal beverages, food flavoring, cosmetics, and pharmaceutical industry. In this paper, chemical compounds of Salvia eriophora (S. eriophora) leaves were determined by LC–MS/MS (Liquid Chromatography tandem Mass Spectrometry). Salvigenin (158.64 ± 10.8 mg/kg), fumaric acid (123.09 ± 8.54 mg/kg), and quercetagetin‐3.6‐dimethylether (37.85 ± 7.09 mg/kg) were detected as major compounds in the ethanol extract, whereas fumaric acid (555.96 ± 38.56 mg/kg), caffeic acid (103.62 ± 20.51 mg/kg), and epicatechin (83.19 ± 8.43 mg/kg) were detected as major compounds in the water extract. Furthermore, enzyme inhibition of S. eriophora against acetylcholinesterase (AChE), α‐amylase (AM), butyrylcholinesterase (BChE), and α‐glycosidase (AG) enzymes were detected. AChE, BChE, AG, and AM enzymes were very strongly inhibited by S. eriophora water extract (WES) and S. eriophora methanol extract (MES). Additionally, antioxidant potential of S. eriophora was determined by in vitro analytical methods. IC50 values of WES and MES were performed for radicals. Practical applications Metabolic enzymes have crucial functions on living systems due to inhibition or activation of them mainly attributed with some health disorders. AChE, BChE, AM, and AG enzymes have important roles on carbohydrate metabolism or cholinergic pathways. The relation between enzyme inhibition effect and phenolic compounds or antioxidant activity need to be confirmed. Thus, many studies tested to clarify this relation for pure samples or plant extracts. To the best of our knowledge, this is the first report about inhibition effects of Salvia eriophora extracts against AChE, BChE, AM, and AG enzymes as well as their phenolic contents and antioxidant activities.

show abstract

Acetylcholinesterase (AChE) and heat shock proteins (Hsp70) of gypsy moth (Lymantria dispar L.) larvae in response to long-term fluoranthene exposure

Cited by 34 publications

References 51 publications

Insecticidal Activity of Artemisia vulgaris Essential Oil and Transcriptome Analysis of Tribolium castaneum in Response to Oil Exposure

Insecticidal Activity of Artemisia vulgaris Essential Oil and Transcriptome Analysis of Tribolium castaneum in Response to Oil Exposure

Organophosphorus Compounds and their Role in Agriculture

Phytochemical content, antioxidant activity, and enzyme inhibition effect ofSalvia eriophoraBoiss. & Kotschy against acetylcholinesterase, α-amylase, butyrylcholinesterase, and α-glycosidase enzymes

Contact Info

Product

Resources

About