Phytochemical content, antioxidant activity, and enzyme inhibition effect ofSalvia eriophoraBoiss. & Kotschy against acetylcholinesterase, α-amylase, butyrylcholinesterase, and α-glycosidase enzymes

Abstract

Many taxa of Salvia genus have been used in herbal beverages, food flavoring, cosmetics, and pharmaceutical industry. In this paper, chemical compounds of Salvia eriophora (S. eriophora) leaves were determined by LC–MS/MS (Liquid Chromatography tandem Mass Spectrometry). Salvigenin (158.64 ± 10.8 mg/kg), fumaric acid (123.09 ± 8.54 mg/kg), and quercetagetin‐3.6‐dimethylether (37.85 ± 7.09 mg/kg) were detected as major compounds in the ethanol extract, whereas fumaric acid (555.96 ± 38.56 mg/kg), caffeic acid (103.62 ± 20.51 mg/kg), and epicatechin (83.19 ± 8.43 mg/kg) were detected as major compounds in the water extract. Furthermore, enzyme inhibition of S. eriophora against acetylcholinesterase (AChE), α‐amylase (AM), butyrylcholinesterase (BChE), and α‐glycosidase (AG) enzymes were detected. AChE, BChE, AG, and AM enzymes were very strongly inhibited by S. eriophora water extract (WES) and S. eriophora methanol extract (MES). Additionally, antioxidant potential of S. eriophora was determined by in vitro analytical methods. IC50 values of WES and MES were performed for radicals. Practical applications Metabolic enzymes have crucial functions on living systems due to inhibition or activation of them mainly attributed with some health disorders. AChE, BChE, AM, and AG enzymes have important roles on carbohydrate metabolism or cholinergic pathways. The relation between enzyme inhibition effect and phenolic compounds or antioxidant activity need to be confirmed. Thus, many studies tested to clarify this relation for pure samples or plant extracts. To the best of our knowledge, this is the first report about inhibition effects of Salvia eriophora extracts against AChE, BChE, AM, and AG enzymes as well as their phenolic contents and antioxidant activities.

Anticholinergic, antidiabetic and antioxidant activities of cinnamon ( cinnamomum verum ) bark extracts: polyphenol contents analysis by LC-MS/MS

“…[78] The ABTS •+ scavenging method is based on a previously described method. [32] The 2.0 mM ABTS solution with 2.3 mM K 2 S 2 O 8 oxidizing agent resulted in ABTS cation radicals (ABTS •+ ). The initial absorbance (0.700 ± 0.025) was adjusted with buffer (0.1 mM; pH = 7.4) at 734 nm.…”

Chemical profile, antiproliferative, antioxidant, and enzyme inhibition activities and docking studies of Cymbopogon schoenanthus (L.) Spreng. and Cymbopogon nervatus (Hochst.) Chiov. from Sudan

“…Many of these plants are widespread throughout the world, and important in African traditional medicine, being used for the treatment of diseases such as malaria, hepatitis, cancer, inflammation, and infections by fungi, bacteria, and viruses (Bursal et al, 2019;Gülçin, Tel, Gören, Taslimi, & Alwasel, 2019;Lawal & Ogunwande, 2013;Tohma et al, 2016). Many of these plants are widespread throughout the world, and important in African traditional medicine, being used for the treatment of diseases such as malaria, hepatitis, cancer, inflammation, and infections by fungi, bacteria, and viruses (Bursal et al, 2019;Gülçin, Tel, Gören, Taslimi, & Alwasel, 2019;Lawal & Ogunwande, 2013;Tohma et al, 2016).…”

“…Aromatic plants of Africa are currently receiving great attention due to their biological and chemical diversities. Many of these plants are widespread throughout the world, and important in African traditional medicine, being used for the treatment of diseases such as malaria, hepatitis, cancer, inflammation, and infections by fungi, bacteria, and viruses (Bursal et al, 2019;Gülçin, Tel, Gören, Taslimi, & Alwasel, 2019;Lawal & Ogunwande, 2013;Tohma et al, 2016).…”

Phytochemical Content, Antidiabetic, Anticholinergic, and Antioxidant Activities of Endemic Lecokia cretica Extracts

“…According to the results of this study, AChE, BChE, and α ‐glycosidase enzymes were efficiently inhibited by L. cretica extract. TAC (9‐amino‐1,2,3,4‐tetrahydroacridine) is a reversible inhibitor of esterase and is the first drug for the palliative treatment of AD . The IC 50 values of L. cretica ethanol extract were found as 3.21 mg/mL for AChE, 2.1 mg/mL for BChE, and 2.07 mg /mL for α ‐glycosidase.…”

“…TAC (9-amino-1,2,3,4-tetrahydroacridine) is a reversible inhibitor of esterase and is the first drug for the palliative treatment of AD. [34] The IC 50 values of L. cretica ethanol extract were found as 3.21 mg/mL for AChE, 2.1 mg/mL for BChE, and 2.07 mg /mL for α-glycosidase. Tacrine was used as a positive standard for BChE and AChE inhibition and had IC 50 values of 19.11 mg/mL and 12.36 mg/mL, respectively.…”