Acetamiprid Induces Toxicity in Mice under Experimental Conditions with Prominent Effect on the Hematobiochemical Parameters

Abstract

The present study was carried out to assess the toxicological effect of acetamiprid, an insecticide of neonicotinoid group on clinical and serum hematobiochemical parameters of mice. The experimental animals were divided into four different groups of equal number of animals and the first group was considered as Control. It was found that acetamiprid administered @ 40 mg/kg body weight per day (1/5 th of LD% value) for a period of 28 days induces prominent toxic symptoms along with hematological and biochemical effect in mice. Acetamiprid induced mice showed decrease in body weight and clinical symptoms like respiratory depression, sitting in the corner, diarrhea, depression, dullness etc. are observed at the different dose levels. Test mice administered with acetamiprid showed significant decrease in overall hematological profile. In the present study, it was found that acetamiprid @ 10 mg/ kg body weight (1/20 th of LD50 value) did not produce significant changes in hematological and biochemical values. So, it was considered that experimental dose of 10 mg/kg to be non-toxic and 40 mg/kg body weight to be the toxic dose of acetamiprid in mice.

HPLC‐DAD Analysis, Antioxidant and Protective Effects of Tunisian Rhanterium suaveolens against Acetamiprid Induced Oxidative Stress on Mice Erythrocytes

“…In fact, the administration of ACT at a dose of 20 mg/kg BW/day for 21 consecutive days caused a significant decrease in red blood cells (RBC) count, hemoglobin (Hb) content and hematocrit (HCT) level which indicates the installation of hemolytic anemia compared to the control group ( Table ). These results were in accordance with previous works …”

“…ACT treated mice showed normocytic normochromic anemia as their MCV (mean corpuscular volume), MCH (mean corpuscular hemoglobin content) and MCHC (mean corpuscular concentration in hemoglobin) values did not significantly differ from controls ( Table ). Our findings were similar to previous researches using different types of xenobiotics, and were reinforced by many reports concerning acetamiprid exposure …”

“… Group 1 (control group) was given distilled water. Group 2 (ACT) was administered via gavage with 20 mg/kg body weight of acetamiprid (1/10 LD 50 of acetamiprid) Group 3 (Rs+ACT) received acetamiprid via gavage with the same dose given to group 2 and Rs extract freshly prepared via drinking distilled water with dose of 300 mg/kg body weight, equivalent to 300 mg in 100 mL (w/v). Group 4 (Rs) was treated with 300 mg/kg body weight, equivalent to 300 mg in 100 mL of Rs extract alone administered via drinking distilled water. …”

“…Group 2 (ACT) was administered via gavage with 20 mg/kg body weight of acetamiprid (1/10 LD 50 of acetamiprid) …”

Hematological, Biochemical and Histological alterations induced by oral administration of Thiamethoxam and Acetamiprid in male rats

“…Our finding were in agreement withmany authorsVohra and Khera, (37) who showedimidacloprid, neonicotinoid, caused reduction in platelet count and disturbed blood clotting.Gawadeet al, (38) reported that imidacloprid reduced in Hb content may be due to increased rate of breakdown of red cells and/or reduction in the rate of formation of RBC's (39) . Furthermore, Singh et al, (40) who suggested that acetamiprid in mice decreased Hb levels. Our finding agreement with Roy and Nath, (41) who showed that THIA caused reduction in RBCs count and Hb level due to the destruction of RBCtriggered by the arrival of THIA into erythrocytes, and WBCs increase during higher doses of THIA attributed to the production of a greater amount of antibodies (42) .…”