Acemannan and Formocresol Pulpotomies in Primary Teeth: a Comparative Histopathological Study

Abstract

weight fractions (AHM) containing native ingredients of polysaccharide (acemannan) and glycoprotein (verectin) were obtained by using the patented hyper dry system after washing out coloured materials with running water from Aloe vera gel slurry. Forty-six primary first teeth from children between 6-9 years, which formerly planned for serial extraction were selected. The children were distributed into two equal groups; twelve teeth for each. Pulpotomy was done in both groups; where group I treated by AHM, acemannan, as a dressing agent while formocresol used as a dressing agent in group II. The teeth from each group were extracted at two, four, and twelve week intervals for histopathological evaluation. RESULTS: In group I (Acemannan), after twelve weeks significantly better results in pulp inflammation, incomplete dentine bridge formation, and soft tissue organization were demonstrated (p < 0.05). Most of the acemannan-treated group demonstrated no pulp inflammation with only (3/12, 25%) revealed mild pulp inflammation under the exposure site and only one tooth 1/12 teeth (8.3%) showed partial dentine bridge formation. In contrast, group II (Formocresol) moderate or severe pulp inflammation (10/12, 83%), no dentine bridge formation were observed. In almost all cases (10/12, 83%) the soft tissue of the pulp was not well organized. CONCLUSION: According to these findings, AHM, acemannan may be a suitable agent for pulpotomy in primary teeth. Moreover, the inflammatory response was less severed and no necrosis was noted after pulpotomy with acemannan compared to formocresol.

Clinical and radiographic evaluation of acemannan as direct pulp capping material in young permanent teeth

“…Another study done by Gonna et al (19) to compared the histopathological effects of acemannan to that of formocresol as pulp-dressing agents in primary teeth that underwent pulpotomy. The success rate of Acemannan and Formocresol groups was (75%) and (25%) respectively.…”

“…If bleeding persisted after this procedure, the tooth was not included in the study. At this point, the tooth was allocated randomly to one of the two experimental groups: Group A: Acemannan was mixed gently with distilled water and gently placed on the pulp exposure site ( 19) , care was taken to avoiding any pressure during placement of the material.…”

Nutraceutical Aloe Vera Gel Scaffolds for Supporting a Healthy Muscle Movement

“…Gonna et al [4] compared the histopathological effects of ACM to that of formocresol as pulp-dressing agents in primary teeth underwent pulpotomy. The authors suggest that ACM sponge may be a suitable agent for pulpotomy in primary teeth, and the inflammatory response was less severed and no necrosis was noted after pulpotomy with ACM compared to formocresol.…”

“…Yagi [37] reported that some of the phytochemicals; such as emodin, aloe-emodin in latex and ACM, aloe lectin (verectin) in inner gel of AV, provide relief to rheumatoid arthritis patients through promoting wound healing as well as reducing inflammation and reliving pain, which are common symptoms of rheumatoid patients. Our dental study [4] on pulpotomy in primary teeth showed that Aloe vera high molecular materials (AHM) could serve as a bioactive molecule that has the capability of bone formation as it stimulates bone marrow stromal cells proliferation, differentiation into osteoblasts. ACM and aloe lectin (verectin) containing in small amount in AV gel, as the controlled releasing active ingredients, may be a potential biomaterial for bone regeneration [4] .…”

“…Our dental study [4] on pulpotomy in primary teeth showed that Aloe vera high molecular materials (AHM) could serve as a bioactive molecule that has the capability of bone formation as it stimulates bone marrow stromal cells proliferation, differentiation into osteoblasts. ACM and aloe lectin (verectin) containing in small amount in AV gel, as the controlled releasing active ingredients, may be a potential biomaterial for bone regeneration [4] . Prophylactic aloe components; aloe-emodin, emodin and fermented butyrate, on autoimmune diseases, such as rheumatoid arthritis, were presented in a previous paper [38] .…”