Accuracy of the VITEK 2 system for a rapid and direct identification and susceptibility testing of Gram-negative rods and Gram-positive cocci in blood samples

Nimer, Nabil Awni; Al-Saa'da, Raeeda; Abuelaish, Omar

doi:10.26719/2016.22.3.193

Cited by 14 publications

(11 citation statements)

References 21 publications

(37 reference statements)

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“…The Vitek 2 system can identify gram-negative rods within 4 h. This system has shown to be an accurate, easy, and rapid tool for the identification of K. pneumoniae isolates [24]. In our study, the Vitek 2 system demonstrated a similar rate of accuracy in the identification of Klebsiella and identified the isolates to the species level.…”

Section: Vol 8 Nosupporting

confidence: 61%

Antibiotic Resistance among Klebsiella pneumoniae Isolates by Detecting blaVIM and blaNDM Genes

Bandari

Keyvani

Abootaleb

2020

JoMMID

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Introduction: The emergence of extended-spectrum β-lactamase (ESBL) and carbapenem-resistant Enterobacteriaceae, especially Klebsiella pneumoniae isolates, has become a severe concern worldwide. This study aimed to determine the prevalence of blaVIM and blaNDM genes among K. pneumoniae isolates. Methods: One hundredeighty-one K. pneumoniae isolates were obtained from different clinical specimens of patients hospitalized at Firoozgar hospital, Tehran, Iran. The isolates were identified by standard biochemical tests, and their identity was confirmed by Vitek 2 (bioMérieux, France), a fully automated system for bacterial identification. The isolates were subjected to antimicrobial susceptibility testing and screened for ESBL by double-disc synergy test (DDST) and modified Hodge test (MHT) for the detection of carbapenemase. PCR was also used to detect the presence of blaVIM and blaNDM resistance genes in the isolates. Results: The Vitek 2 system confirmed the biochemical test results. The highest and lowest rates of resistance to antibiotics belonged to cefepime (83.9%) and imipenem (55.2%). Eighty-six and 100 isolates showed to produce ESB and KPC by DDST and MHT, respectively. About 71% and 97% of the 100 isolates were positive for blaVIM and blaNDM genes, respectively. Conclusion: The high rate of ESBL-and KPC-producing K. pneumoniae isolates in our hospital setting revealed resistance to conventional antibiotics, which limit our options in choosing appropriate antimicrobials. Although the management of infections associated with these organisms is challenging, it is essential to control such strains to prevent the outbreak.

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Section: Vol 8 Nosupporting

confidence: 61%

Antibiotic Resistance among Klebsiella pneumoniae Isolates by Detecting blaVIM and blaNDM Genes

Bandari

Keyvani

Abootaleb

2020

JoMMID

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“…Bacterial isolates were identified based on cultural and biochemical criteria. The method followed was adapted from a previous study that reported that VITEK2 cards inoculated with fluids sampled directly from culture bottles are suitable for speedy identification and susceptibility testing [15]. A sterile swab or applicator stick was used to transfer a sufficient number of colonies of a pure culture in 3.0 mL of sterile saline (0.45% NaCl, pH 4.5-7.0) in a 12×75 mm clear plastic (polystyrene) test tube.…”

Section: Methodsmentioning

confidence: 99%

“…A sterile swab or applicator stick was used to transfer a sufficient number of colonies of a pure culture in 3.0 mL of sterile saline (0.45% NaCl, pH 4.5-7.0) in a 12×75 mm clear plastic (polystyrene) test tube. The turbidity was adjusted to 0.50-0.63 Mf and measured using a turbidity meter known as DensiChek [15]. Results were read overnight.…”

Section: Methodsmentioning

confidence: 99%

Molecular and phylogenetic study of Staphylococcus aureus isolated from human and cattle of Al-Qadisiyah Governorate, Iraq

et al. 2019

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Aim:This study was designed to detect the prevalence of Staphylococcus aureus, to estimate the frequency of methicillin resistance gene (mecA), femA (specific gene for S. aureus), and lukS gene, and the prevalence of urinary tract infection (UTI) in human and bovine mastitis caused by S. aureus.Materials and Methods:A total of 102 cases of S. aureus were included in this study; 72 specimens were isolated from human with UTIs and 30 specimens were isolated from milk of cattle with acute mastitis. Diagnosis was done by VITEK 2 Compact after subculture and purification. All isolates were examined for the presence of mecA, femA, and lukS (Panton-Valentine leukocidin) using multiplex polymerase chain reaction.Results:Culture and biochemical evaluation of the samples revealed the presence of S. aureus, among which the genes mecA, femA, and lukS were positively detected in 68 (94.4%), 36 (50%), and 20 (27.7%) of S. aureus isolates from methicillin-resistant humans, respectively. In the same manner, the genes mecA, femA, and lukS were positively detected in 27 (90%), 14 (46.7%), and 11 (36.7%) of S. aureus isolates from methicillin-resistant cattle. Sequencing of partial order of femA gene isolated from human isolate and from cattle with mecA isolated from human revealed high sequence identity with the National Center for Biotechnology Information (NCBI)-Basic Local Alignment Search Tool. S. aureus isolates and the phylogenetic analysis showed that there was a significant genetic similarity (0.5 genetic change) between human and animals isolates, and then, the gene sequences were deposited into NCBI-Genbank accession numbers MG696860.1 for mecA and femA from human, MG696861.1 for mecA and femA from cattle, MK474469.1 for mecA and femA gene from human, and MG696862.1 for mecA and femA gene from cattle.Conclusion:The study represents the first report of genetic relationship between S. aureus from humans and cattle of Iraq. Therefore, it is essential to define the role of animals as an important source of the distribution of pathogen related to public health. The continuous monitoring of methicillin susceptibility pattern of S. aureus isolates that have high standards of infections might prevent methicillin-resistant S. aureus transmission in either direction between human and cattle, the risk of dairy milk on humans, or self-direction between the same species.

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“…Marcy l’Etoile, France) used in the present study is an automated system for identification and antimicrobial susceptibility testing after a standardized inoculum has been loaded into the system. The results are available within 3 h for identification and 18 h for susceptibility testing [ 15 ]. However, the crucial need for speeding up the process of identification and antimicrobial susceptibility of the positive blood cultures lead to more recent advances that allow more rapid identification within 1 h. The BioFire FilmArray blood culture ID (BCID2; bioMérieuxm Marcy l’Etoile, France) is a highly multiplexed PCR kit that identifies 33 species, including 28 bacterial species and five Candida species.…”

Section: Introductionmentioning

confidence: 99%

BioFire FilmArray BCID2 versus VITEK-2 System in Determining Microbial Etiology and Antibiotic-Resistant Genes of Pathogens Recovered from Central Line-Associated Bloodstream Infections

Sherif

Elsayed

El-Ansary

et al. 2022

Biology

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Central line-associated bloodstream infection (CLABSI) is among the most serious hospital acquired infections. Therefore, the rapid detection of the causative microorganism is of crucial importance to allow for the appropriate antimicrobial therapy. In the present study, we analyzed the clinical performance of the BioFire FilmArray Blood Culture Identification 2 (BCID2) panel in the identification of 33 microbial species and 10 antibiotic resistance genes in comparison to the VITEK-2 system. A total of 104 blood specimens were included. The FilmArray BCID2 results were concordant with the VITEK-2 system in 69/97 specimens (71.1%). Non-concordance was either due to the detection of more pathogens by the FilmArray BCID2 23/28 (82%) or microbial species were misidentified 5/28 (18%). Hence, in comparison to the VITEK-2 system, the FilmArray BCID2 panel showed an overall sensitivity of 75.8% (95% CI, 66–83%) and an overall specificity of 98% (95% CI, 97–98.8%) in detecting microbial species. For the resistance genes, the FilmArray BCID was able to detect the presence of blaCTX-M gene in 23 Gram-negative isolates, blaNDM and blaOXA-48- like genes in 14 and 13 isolates, respectively. The mecA and mecC genes were found in 23 Staphylococcus species, while mecA, mecC and MREJ genes were found in 4 Staphylococcus aureus isolates. The sensitivity and specificity for detecting resistance genes by the FilmArray BCID2 was 90% (95% CI, 81.4–95%) and 99.6% (95% CI, 99–100%), respectively. As concluded, the present study emphasizes the high sensitivity and specificity of the FilmArray BCID2 in the rapid and reliable detection of different bacteria and fungi from positive blood culture bottles, as well as the accurate detection of various antibiotic resistance markers.

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Accuracy of the VITEK 2 system for a rapid and direct identification and susceptibility testing of Gram-negative rods and Gram-positive cocci in blood samples

Cited by 14 publications

References 21 publications

Antibiotic Resistance among Klebsiella pneumoniae Isolates by Detecting blaVIM and blaNDM Genes

Antibiotic Resistance among Klebsiella pneumoniae Isolates by Detecting blaVIM and blaNDM Genes

Molecular and phylogenetic study of Staphylococcus aureus isolated from human and cattle of Al-Qadisiyah Governorate, Iraq

BioFire FilmArray BCID2 versus VITEK-2 System in Determining Microbial Etiology and Antibiotic-Resistant Genes of Pathogens Recovered from Central Line-Associated Bloodstream Infections

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