Burns are the most prevalent type of trauma in the world, and they have a high fatality rate. For cutaneous wound healing, modern and natural therapies, particularly probiotic supplements, have lately been considered. The goal of this study was to see how Lactiplantibacillus plantarum affected wound healing as well as the antibacterial activity of probiotic lactobacilli against Pseudomonas aeruginosa. The glass slide method was used to assess anti-adhesion activity, and the HPLC method was used to quantify anti-adhesion chemicals in cell-free supernatant (CFS). MDR P. aeruginosa was administered subcutaneously directly on the burn after induction of second-degree wounds. Three groups of animals were created. Every day, the supernatants were sprayed for therapy, and the wound healing was monitored. Lactobacilli bacteria had good anti-adhesion effects on P. aeruginosa, according to our findings, and HPLC research revealed that their inhibitory effect could be attributable to four main organic acids: lactic acid, acetic acid, citric acid, and succinic acid. When the effect of treatments on fibroblastic cells was examined, it was discovered that the group treated with L. plantarum supernatants had the most fibroblastic cells when compared to the non-treated group. Furthermore, the bacteria increased the number of fibroblastic cells, re-epithelialization in the wound area, and the thickness of the epidermis and dermis layers. Lactobacilli bacteria's antimicrobial activity against MDR P. aeruginosa was determined by prevents infection. These findings revealed that L. plantarum can treat a P. aeruginosa infection in a second-degree burn and can significantly reduce inflammation.
Background:The prevalent emergence of carbapenem-resistant Enterobacteriaceae, especially Klebsiella pneumoniae carbapenemases (KPC) that causes infection associated with multidrug-resistant, is a major clinical and public health concern. Accurate and fast detection of carbapenemases is essential for effective infection control. Objectives: The aims of this study were the detection of blaKPC and blaGES genes with phenotypic and genotypic methods, evaluation of expression level of these genes in the presence and absence of β-lactam antibiotic, and determination of antibiotic resistance patterns among K. pneumoniae isolated at Firoozgar Hospital. Methods: One hundred and eighty-one K. pneumoniae strains were collected from patients presenting to Firoozgar Hospital of Tehran, Iran from March 2018 to December 2018. The strains were tested using the disk diffusion method, modified Hodge test (MHT), and minimum inhibitory concentration (MIC). The presence of blaKPC and blaGES resistance genes was detected by RT-PCR.The blaKPC and blaGES genes expression level was measured by real-time PCR in the presence and absence of β-lactam antibiotic. Results:The results of this study showed the highest and lowest rates of resistance to cefepime and imipenem were 83.9% and 55.2%, respectively. 100 strains were positive as KPC-producing in MHT. Also, they exhibited resistance to imipenem by E-test. 51% and 49% of these 100 isolates were positive for blaKPC and blaGES genes, respectively. Real-time PCR assay showed the higher expression level of blaKPC (1.04 folds) and blaGES (12.21 folds) increase in resistant strains in the presence of imipenem. Conclusions: Due to the high resistance of K. pneumoniae isolates to common antibiotics, hence, there is an urge for revisiting the antibiotic therapy protocols for prevention and control of the spread of resistant bacteria.
Background:The prevalence of carbapenem-resistant Enterobacteriaceae, especially Klebsiella pneumoniae carbapenemase (KPC), has been recently reported worldwide. Therefore, there is an indispensable need for precise and rapid detection of these carbapenemases. Objectives: This study was aimed to propose an accurate and rapid method for detecting K. pneumoniae carbapenemase genes from clinical samples, using reverse transcriptionpolymerase chain reaction (RT-PCR) and to evaluate the expression of these genes in the presence of β-lactam antibiotic by real-time PCR assay. Methods: One hundred and eighty-one K. pneumoniae strains were collected from patients presenting to Firoozgar Hospital of Tehran, Iran. The strains were tested using the disk diffusion method, the modified Hodge test (MHT), and E-test minimum inhibitory concentration (MIC). Next, reverse transcription-PCR method was applied for the identification of bla OXA-23 and bla OXA-48 genes. Finally, expression of genes was measured by real-time PCR assay in the presence and absence of β-lactam antibiotic. Results:Phenotypic testing showed a high level of antibiotic resistance, while the genotypic methods indicated the presence and expression of carbapenemase genes. Conclusions: The findings suggest revisions in the current antibiotic therapy protocols, considering the high expression level of resistant carbapenemases to K. pneumoniae strains.
Introduction: The emergence of extended-spectrum β-lactamase (ESBL) and carbapenem-resistant Enterobacteriaceae, especially Klebsiella pneumoniae isolates, has become a severe concern worldwide. This study aimed to determine the prevalence of blaVIM and blaNDM genes among K. pneumoniae isolates. Methods: One hundredeighty-one K. pneumoniae isolates were obtained from different clinical specimens of patients hospitalized at Firoozgar hospital, Tehran, Iran. The isolates were identified by standard biochemical tests, and their identity was confirmed by Vitek 2 (bioMérieux, France), a fully automated system for bacterial identification. The isolates were subjected to antimicrobial susceptibility testing and screened for ESBL by double-disc synergy test (DDST) and modified Hodge test (MHT) for the detection of carbapenemase. PCR was also used to detect the presence of blaVIM and blaNDM resistance genes in the isolates. Results: The Vitek 2 system confirmed the biochemical test results. The highest and lowest rates of resistance to antibiotics belonged to cefepime (83.9%) and imipenem (55.2%). Eighty-six and 100 isolates showed to produce ESB and KPC by DDST and MHT, respectively. About 71% and 97% of the 100 isolates were positive for blaVIM and blaNDM genes, respectively. Conclusion: The high rate of ESBL-and KPC-producing K. pneumoniae isolates in our hospital setting revealed resistance to conventional antibiotics, which limit our options in choosing appropriate antimicrobials. Although the management of infections associated with these organisms is challenging, it is essential to control such strains to prevent the outbreak.
Background: The immune antigen of Bacillus anthracis is a protein that can attach to the surface receptor of all human cells. At the surface of cancer cells, there is a receptor that activates the uPA (Urokinase plasminogen) that do not exist in normal human cells. Objectives: The aim of this study was changing the location of the attachment of the PA gene by a directed mutation in order to attach only to the cancer cells. Methods: PA gene was extracted from the pMNA1 plasmid. The mutation on the PA gene was made by Overlap Extension PCR. The mutated segment was transferred to DH5α; the strain of Escherichia coli. With TA coning carrier. By restriction enzymes Hind III and BamH I the mutated PA gene was extracted and transferred to pWB980 and by electroporation method, it was transferred to the WB600 strain. Results: In this study, the mutation occurred in sequences of PA gene by SOE PCR method resulting in a change in the genetic code of amino acid 194. The occurrence of mutation was confirmed by determining base sequences. Conclusion: Cancer is a severe disease that has a major impact on large groups of people, about whom the problem of cancer is a leading cause of death across the world. One of the treatment methods of cancer is bacterial toxins if only cancer cells receive them. Therefore, these mutated PA proteins can be effective as novel therapeutic agents for the treatment of cancer.
Background: Camel milk is the most important of dairy foods. Its contains amino acids, vitamins, probiotics properties and a potential source for isolation of probiotic Lactobacillus strains. This study is aimed to identify and isolate the bacteria special Lactic Acid Bacteria in the camel milk based on the molecule methods. Methods: All samples were collected from camel milk in Semnan province of Iran. Initially, they were cultivated in MRS Agar. Plates were incubated by 37 °C for 48 hours. Bacteria identification was done according to interior transcription of the area 16 SrRNA. The products of PCR were successfully determined and were analyzed. Finally, a phylogenetic tree was constructed by using Clustal Omega. Results: The observed bacteria were gram-positive, catalase-negative rods or cocci and vancomycin-resistant. Following that they identified as E. gallinarum and E. casseliflavus by analytical results ribosomal DNA sequencing with 100% similarity. Also, a phylogenetic tree is proven the species relatedness of the Enterococcus spp. Conclusions: These findings showed that supporting 16S rRNA sequences is a reasonable technique for identifying Lactobacillus strains. Also, isolated bacteria are a strong candidate for using in food and pharmaceutical industry.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.