Burns are the most prevalent type of trauma in the world, and they have a high fatality rate. For cutaneous wound healing, modern and natural therapies, particularly probiotic supplements, have lately been considered. The goal of this study was to see how Lactiplantibacillus plantarum affected wound healing as well as the antibacterial activity of probiotic lactobacilli against Pseudomonas aeruginosa. The glass slide method was used to assess anti-adhesion activity, and the HPLC method was used to quantify anti-adhesion chemicals in cell-free supernatant (CFS). MDR P. aeruginosa was administered subcutaneously directly on the burn after induction of second-degree wounds. Three groups of animals were created. Every day, the supernatants were sprayed for therapy, and the wound healing was monitored. Lactobacilli bacteria had good anti-adhesion effects on P. aeruginosa, according to our findings, and HPLC research revealed that their inhibitory effect could be attributable to four main organic acids: lactic acid, acetic acid, citric acid, and succinic acid. When the effect of treatments on fibroblastic cells was examined, it was discovered that the group treated with L. plantarum supernatants had the most fibroblastic cells when compared to the non-treated group. Furthermore, the bacteria increased the number of fibroblastic cells, re-epithelialization in the wound area, and the thickness of the epidermis and dermis layers. Lactobacilli bacteria's antimicrobial activity against MDR P. aeruginosa was determined by prevents infection. These findings revealed that L. plantarum can treat a P. aeruginosa infection in a second-degree burn and can significantly reduce inflammation.
Background: Microorganisms attach to various surfaces and they have manufactured biofilms by production polysaccharides like PSL in P. aeruginosa. Synthesis of this kind of polysaccharide has done by PSL gene cluster. The aim of this study is consideration of biofilm formation which is one of the major cause of antibiotic resistance. Methods: In this study, 100 P. aeruginosa were isolated with bacteriological and biochemical methods and pslA gene detection with PCR in all of the P. aeruginosa isolated from patients. Then biofilm formation checked with microtiter plate method and it showed with SEM. Finally, expression of main attachment gene pslA in 6 strains could make moderate and strong biofilm were investigated by real-time PCR assay. Results: In this study, 100 P. aeruginosa were isolated that these strains showed high rates of MDR. The presence pslA gene in all of the pseudomonas isolated from patients was proven. Microtiter plate method showed 24 (24%) strains could make biofilm among 100 strains that showed with SEM. The pslA expression in strains which making moderate and strong biofilms are more than other strains. Conclusions: Hence, for bacterial biofilm treatment is recommended: before antibiotics are prescribed, biofilm formation by bacteria should be investigated.
Background: The immune antigen of Bacillus anthracis is a protein that can attach to the surface receptor of all human cells. At the surface of cancer cells, there is a receptor that activates the uPA (Urokinase plasminogen) that do not exist in normal human cells. Objectives: The aim of this study was changing the location of the attachment of the PA gene by a directed mutation in order to attach only to the cancer cells. Methods: PA gene was extracted from the pMNA1 plasmid. The mutation on the PA gene was made by Overlap Extension PCR. The mutated segment was transferred to DH5α; the strain of Escherichia coli. With TA coning carrier. By restriction enzymes Hind III and BamH I the mutated PA gene was extracted and transferred to pWB980 and by electroporation method, it was transferred to the WB600 strain. Results: In this study, the mutation occurred in sequences of PA gene by SOE PCR method resulting in a change in the genetic code of amino acid 194. The occurrence of mutation was confirmed by determining base sequences. Conclusion: Cancer is a severe disease that has a major impact on large groups of people, about whom the problem of cancer is a leading cause of death across the world. One of the treatment methods of cancer is bacterial toxins if only cancer cells receive them. Therefore, these mutated PA proteins can be effective as novel therapeutic agents for the treatment of cancer.
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