Accumulation of Polyhydroxyalkanoate from Styrene and Phenylacetic Acid by Pseudomonas putida CA-3

Abstract

Pseudomonas putida CA-3 is capable of converting the aromatic hydrocarbon styrene, its metabolite phenylacetic acid, and glucose into polyhydroxyalkanoate (PHA) when a limiting concentration of nitrogen (as sodium ammonium phosphate) is supplied to the growth medium. PHA accumulation occurs to a low level when the nitrogen concentration drops below 26.8 mg/liter and increases rapidly once the nitrogen is no longer detectable in the growth medium. The depletion of nitrogen and the onset of PHA accumulation coincided with a decrease in the rate of substrate utilization and biochemical activity of whole cells grown on styrene, phenylacetic acid, and glucose. However, the efficiency of carbon conversion to PHA dramatically increased once the nitrogen concentration dropped below 26.

Polyphosphate Accumulation by Pseudomonas putida CA-3 and Other Medium-Chain-Length Polyhydroxyalkanoate-Accumulating Bacteria under Aerobic Growth Conditions

“…Previous studies have reported the accumulation of polyP by Escherichia coli in response to nitrogen limitation (3,11). As previously reported, negligible levels (0.3% CDW) of mclPHA were observed in P. putida CA-3 cells 5 h after inoculation (31) (Fig. 2).…”

Plant Oils and Products of Their Hydrolysis as Substrates for Polyhydroxyalkanoate Synthesis

“…A number of Pseudomonas strains (P. putida KT2440, CA-3 and GO16, P. chlororaphis 555) were tested for their ability to grow and produce PHA on a range of plant oils. The Pseudomonas putida strains have previously been used to produce mcl-PHA from a variety of substrates 34,35,36 . Other P. chlororaphis strains have been used to produce mcl-PHA from palm kernel oil 14 .…”

GacS-Dependent Regulation of Polyhydroxyalkanoate Synthesis in Pseudomonas putida CA-3

“…6). Samples were obtained from cultures grown under PHA accumulation conditions previously described (19,28). A protein of ϳ60 kDa in size which is present in wild-type CA-3 (Fig.…”