Acclimatisation-induced stress influenced host metabolic and gut microbial composition change

Abstract

Understanding the basal gut bacterial community structure and the host metabolic composition is pivotal for the interpretation of laboratory treatments designed to answer questions pertinent to host-microbe interactions. In this study, we report for the first time the underlying gut microbiota and systemic metabolic composition in BALB/c mice during the acclimatisation period. Our results showed that stress levels were reduced in the first three days of the study when the animals were subjected to repetitive handling daily but the stress levels were increased when handling was carried out at lower frequencies (weekly). We also observed a strong influence of stress on the host metabolism and commensal compositional variability. In addition, temporal biological compartmental variations in the responses were observed. Based on these results, we suggest that consistency in the frequency and duration of laboratory handling is crucial in murine models to minimise the impact of stress levels on the commensal and host metabolism dynamics. Furthermore, caution is advised in consideration of the temporal delay effect when integrating metagenomics and metabonomics data across different biological matrices (i.e. faeces and urine).

Probiotic Lactobacillus Paracasei Expressing a Nucleic Acid-Hydrolyzing Minibody (3D8 Scfv) Enhances Probiotic Activities in Mice Intestine as Revealed by Metagenomic Analyses

“…In addition, some studies have demonstrated the influence of dietary ingredients on the fecal microbial community in mice [ 27 , 28 ]. Moreover, the change of mouse intestinal microbiota in response to different environmental changes such as irradiation [ 29 ], acclimatization-induced stress [ 30 ], and exposure to arsenic and iron [ 31 ] has been studied. Gut microbiota from mice with different genetic backgrounds such as wildtype mice and gnotobiotic mice have also been previously examined [ 32 , 33 ].…”

Coffee consumption revealed sex differences in host endogenous metabolism and gut microbiota in healthy adults

“…This study was approved by International Medical University Joint Research and Ethics Committee (IMU MREC number: BP 1-01/12 (09) 2015). The spectra were acquired according to parameters used previously (Low et al, 2017;Yap et al, 2015…”

Temporal dynamics of the gut microbiome and metabolome in preterm and term infants from birth through the first year of life

“…To identify the association between the clinical and demographic parameters with microbial assemblage pattern and changes in metabolic profiles, we conducted DISTLM as described in Yap et al. (70). In brief, the clinical and demographic parameters were selected and fitted to the overall changes in gut microbial composition and metabolic profiles using stepwise regression under the second-order bias-corrected Akaike Information Criterion (AIC).…”

“…A total of 600 µL of supernatant was transferred to a 5 mm (outer diameter) NMR tube (Norell, USA) for NMR analysis.A standard 1-dimensional (1D) 1H-NMR spectrum was acquired for each sample with a pulse [recycle delay (RD)-90º-t1-90º-tm-90º-acquire free induction decay (FID)] on a Bruker 600 MHz spectrometer (Bruker Biospin, Fallenden, Switzerland) with a 5 mm BB(F)O broadband probe operating at 600.13MHz (ambient probe temperature 27 °C). The spectra were acquired according to parameters used previously(70). The field frequency was locked on D2O solvent and water peak suppression was performed by gradient water pre-saturation during RD of 4 s and a mixing time (tm) of 0.01 s. The 90°p ulse length was adjusted to ~10 μs and an acquisition time of 2.65 s was used.…”