Absolute Quantification of Isoflavones in the Flowers of Pueraria lobata by qHNMR

Thapa, Punam; Kim, Hye Mi; Hong, Jongki; Kim, Ranhee; Paudel, Sunil Babu; Choi, Hyukjae; Jang, Dae Sik; Nam, Joo‐Won

doi:10.3390/plants11040548

Cited by 8 publications

(7 citation statements)

References 30 publications

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“…Pueraria lobata is an abundant source of isoflavones ( Yao et al, 2021 ). Here, six active isoflavones were examined and identified as puerarin, daidzein, daidzin, genistein, rutin and apigenin in FPE using LC-MS techniques, which is consistent with previous studies ( Liu et al, 2012 ; Thapa et al, 2022 ). Network pharmacology analysis of the active components of Pueraria lobata for the treatment of IBD revealed that the biological activity of Pueraria lobata was also mainly reflected in isoflavones ( Supplementary Table S1 ).…”

Section: Discussionsupporting

confidence: 88%

Flos puerariae ameliorates the intestinal inflammation of Drosophila via modulating the Nrf2/Keap1, JAK-STAT and Wnt signaling

Yang

Xiu

et al. 2022

Front. Pharmacol.

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Gut homeostasis is important for human health, and its disruption can lead to inflammatory bowel disease (IBD). Flos Puerariae is a herb with a wide variety of pharmacological activities including antioxidant, antidiabetic, antialcoholismic and anti-inflammatory properties. However, the role of Flos Puerariae on treating IBD remains obscure. Here, we employed Drosophila melanogaster as a model organism to investigate the protective effect of Flos Puerariae extract (FPE) against sodium dodecyl sulfate (SDS)-induced intestinal injury. Our data showed that FPE had no toxic effect in flies, and significantly extended lifespan in SDS-inflamed flies, reduced stem cell proliferation in the midgut, and maintained intestinal morphological integrity. Furthermore, FPE remarkably recused the altered expression level of genes and proteins in Nrf2/Keap1 signaling, JAK-STAT signaling and Wnt signaling pathways in gut of inflammation flies. Thus, FPE has a protective effect against intestinal injury possibly via increasing the Nrf2/keap1 pathway and suppressing the JAK-STAT and Wnt signaling pathways, which would have tremendous potential for treating IBD.

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Section: Discussionsupporting

confidence: 88%

Flos puerariae ameliorates the intestinal inflammation of Drosophila via modulating the Nrf2/Keap1, JAK-STAT and Wnt signaling

Yang

Xiu

et al. 2022

Front. Pharmacol.

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“…For instance, the tank is too big to create an ultrasonic blank region, which negatively impacts the effectiveness of the extraction process. Punam [ 46 ] employed several extraction techniques and methanol as the extraction solvent to extract isoflavones from KR flowers. Comparisons were made between the methanol ultrasonic extract (PLs) and the methanol reflux extract (PLr).…”

Section: Sample Pretreatment Methodsmentioning

confidence: 99%

“…Additionally, semi-preparative HPLC is a quick and efficient separation technique for high-purity puerarin isoflavones. Using semi-preparative reverse-phase HPLC, Punam [ 46 ] isolated and purified isoflavones from the flowers of KR. When isoflavones from KR flowers were extracted using various techniques, the concentration ranged from 7.99% to 10.57%.…”

Section: Sample Pretreatment Methodsmentioning

confidence: 99%

Advances in Extraction, Purification, and Analysis Techniques of the Main Components of Kudzu Root: A Comprehensive Review

Xuan,

Liu,

Liu

et al. 2023

Molecules

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Kudzu root (Pueraria lobate (Willd.) Ohwi, KR) is an edible plant with rich nutritional and medicinal values. Over the past few decades, an ample variety of biological effects of Pueraria isoflavone have been evaluated. Evidence has shown that Pueraria isoflavone can play an active role in antioxidant, anti-inflammatory, anti-cancer, neuroprotection, and cardiovascular protection. Over 50 isoflavones in kudzu root have been identified, including puerarin, daidzein, daidzin, 3′-hydroxy puerarin, and genistein, each with unambiguous structures. However, the application of these isoflavones in the development of functional food and health food still depends on the extraction, purification and identification technology of Pueraria isoflavone. In recent years, many green and novel extraction, purification, and identification techniques have been developed for the preparation of Pueraria isoflavone. This review provides an updated overview of these techniques, specifically for isoflavones in KR since 2018, and also discusses and compares the advantages and disadvantages of these techniques in depth. The intention is to provide a research basis for the green and efficient extraction, purification, and identification of Pueraria isoflavone and offers investigators a valuable reference for future studies on the KR.

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“…Its tuber and leaves contain significant phytochemicals and extracts that have historically been used in medicine and nutrition, such as puerarin, daidzein, daidzin, iris, and irisin [ 4 ]; [ 2 ]. These phytochemicals have benefits that include improved immunity, blood lipid control, hypoglycemia, anti-arrhythmia, anti-hepatotoxic, anti-oxidant, anti-mutagenic, anti-myocardial ischemia, and anti-dipsotropic [ 5 ]; [ 6 ]. In studies with alcohol-induced inflammatory conditions, researchers elucidated several plant-derived additives capable of lessening the impact of congeners [ 7 ]; Kudzu showed the best congener-impact lessening results due to its flavonoids contents (amongst other health benefits).…”

Section: Introductionmentioning

confidence: 99%

Ultrafine comminution-assisted ultrasonic-microwave synergistic extraction of Pueraria mirifica (Kudzu flower and root) flavonoids

Zhu,

Xing,

Akan

et al. 2023

Heliyon

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Absolute Quantification of Isoflavones in the Flowers of Pueraria lobata by qHNMR

Cited by 8 publications

References 30 publications

Flos puerariae ameliorates the intestinal inflammation of Drosophila via modulating the Nrf2/Keap1, JAK-STAT and Wnt signaling

Flos puerariae ameliorates the intestinal inflammation of Drosophila via modulating the Nrf2/Keap1, JAK-STAT and Wnt signaling

Advances in Extraction, Purification, and Analysis Techniques of the Main Components of Kudzu Root: A Comprehensive Review

Ultrafine comminution-assisted ultrasonic-microwave synergistic extraction of Pueraria mirifica (Kudzu flower and root) flavonoids

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