Absolute Quantification of Gold Nanoparticles with Femtomolar Accuracy Using Inductively Coupled Plasma Atomic Emission Spectroscopy

McCarthy, L. V.; Dye, Andrew; Ferrari, Enrico

doi:10.1007/978-1-0716-0319-2_21

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Three replicate experiments were done, and the mean (ppm) standard deviation was determined. [17][18][19][20]…”

Section: Characterization Techniques Ultraviolet (Uv)-visible Analysismentioning

confidence: 99%

Tinospora cordifolia Stem Extract-mediated Green Synthesis of Selenium Nanoparticles and its Biological Applications

Puri¹,

Patil²

2022

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Background: According to a literature review Tinospora cordifolia possess alkaloids, tannins, flavonoids, glycosides, and steroids with anti-cancer potential. However, there is a lack of scientific evidence on the green synthesis of selenium nanoparticles from the stem of T. cordifolia and its biological activities. Objectives: This study is intended to synthesize Selenium Nanoparticles using T. cordifolia stem extract, characterise them, and evaluate them in-vitro for antioxidant and anticancer activity. Materials and Methods: The T. cordifolia Selenium Nanoparticles (TC-SeNPs) were synthesised by reducing selenious acid with T. cordifolia stem extract. TC-SeNPs were characterized by UV-Vis, FTIR, XRD, FE-SEM, EDAX and ICP-AES, further evaluated for in-vitro antioxidant activity by DPPH free radical scavenging test and reducing power capacity. The MTT assay of TC-SeNP was carried out in-vitro in the human breast cancer cells line (MCF-7) to determine the anticancer activity. Results:The UV-spectroscopy of TC-SeNPs showed maximum absorbance at 285 nm, which is the characteristic feature of SeNP. According to DLS, SEM, and TEM images, the size of TC-SeNPs was between 100-200 nm and was stable with a Zeta potential of -23.9mV. XRD analysis confirmed the crystallinity of TC-SeNPs. Selenium in TC-SeNP was verified in EDAX analysis (63.36% weight) and ICP-AES 68.08 ±12.2 µg/ml selenium content. The IC 50 value 28.62 ± 0.63 µg/mL and EC 50 (62.50 ± 1.21 µg/mL) values indicated that the TC-SeNPs possess significant antioxidant capacity. MTT assay showed TC-SeNPs were cytotoxic to MCF-7 cell with IC 50 values (31.29 ± 0.22µg/mL), suggesting that TC-SeNPs display moderate cytotoxicity that could dose-dependently inhibit cell proliferation. Conclusion: Thus, experimental evidence provides insight into the successful synthesis of the selenium nanoparticle from the stem of T. cordifolia, its potential therapeutic value, and the prospect of developing an anti-cancer TC-SeNPs formulation.

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“…Three replicate experiments were done, and the mean (ppm) standard deviation was determined. [17][18][19][20]…”

Section: Characterization Techniques Ultraviolet (Uv)-visible Analysismentioning

confidence: 99%

Tinospora cordifolia Stem Extract-mediated Green Synthesis of Selenium Nanoparticles and its Biological Applications

Puri¹,

Patil²

2022

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“…Internal standard solution (2.45 mL of 4 ppb iridium (Sigma-Aldrich), 3% (v/v) HCl in H 2 O) was added to the samples and the resulting solution was measured on a NexION 300× ICP-MS instrument (PerkinElmer) and quantitated using a calibration curve (Figure 4). Alternatively, usGNP uptake was measured using inductively coupled plasma-atomic emission spectroscopy (ICP-AES, Figure 5) using the iCAP 7400 ICP-AES Analyzer (Thermo Fisher Scientific) [47].…”

Section: Cellular Uptake Of Usgnpsmentioning

confidence: 99%

Targeting Ultrasmall Gold Nanoparticles with cRGD Peptide Increases the Uptake and Efficacy of Cytotoxic Payload

et al. 2022

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Cyclic arginyl-glycyl-aspartic acid peptide (cRGD) peptides show a high affinity towards αVβ3 integrin, a receptor overexpressed in many cancers. We aimed to combine the versatility of ultrasmall gold nanoparticles (usGNP) with the target selectivity of cRGD peptide for the directed delivery of a cytotoxic payload in a novel design. usGNPs were synthesized with a modified Brust-Schiffrin method and functionalized via amide coupling and ligand exchange and their uptake, intracellular trafficking, and toxicity were characterized. Our cRGD functionalized usGNPs demonstrated increased cellular uptake by αVβ3 integrin expressing cells, are internalized via clathrin-dependent endocytosis, accumulated in the lysosomes, and when loaded with mertansine led to increased cytotoxicity. Targeting via cRGD functionalization provides a mechanism to improve the efficacy, tolerability, and retention of therapeutic GNPs.

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Emission spectroscopy with coded apertures for enhanced dimensionality

Richardson

2021

Journal of Applied Physics

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A coded aperture is used to demonstrate emission spectroscopy from multiple one-dimensional measurement locations simultaneously with a single camera. The coded aperture mask has several columns of periodic apertures, each with a unique spatial frequency. Light transmitted through all mask columns is detected through an imaging spectrometer. Dispersed light from the various mask columns overlaps on the spectrometer camera but is separated using Fourier-domain filtering using the known spatial frequencies of the mask. As the coded aperture is placed at an image plane, each Fourier-filtered spectrogram comes from a unique one-dimensional measurement location. This technique represents a significant increase in the amount of spatially and spectrally resolved emission data available using a single emission spectrometer and camera at the expense of some spatial resolution due to the Fourier filtering. This instrument is particularly useful for studying transient, non-repeating events. Megahertz-rate emission spectroscopy from five one-dimensional measurement locations is demonstrated with explosive fireballs using a single camera. Optical design parameters and instrument performance characteristics are discussed.

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Absolute Quantification of Gold Nanoparticles with Femtomolar Accuracy Using Inductively Coupled Plasma Atomic Emission Spectroscopy

Cited by 3 publications

References 10 publications

Tinospora cordifolia Stem Extract-mediated Green Synthesis of Selenium Nanoparticles and its Biological Applications

Tinospora cordifolia Stem Extract-mediated Green Synthesis of Selenium Nanoparticles and its Biological Applications

Targeting Ultrasmall Gold Nanoparticles with cRGD Peptide Increases the Uptake and Efficacy of Cytotoxic Payload

Emission spectroscopy with coded apertures for enhanced dimensionality

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