Abnormalities of in vitro immunoglobulin synthesis by peripheral blood lymphocytes from untreated patients with Hodgkin's disease.

Abstract

A B S T R A C T The immunoglobulin-synthesizing activities of peripheral blood mononuclear cells from 57 untreated patients with Hodgkin's disease and 47 normal subjects were compared. Cumulative amounts of IgM and IgG synthesized and secreted by unstimulated and pokeweed mitogen-stimulated cells over a 7-d period were determined in a solid-phase radioimmunoassay. Synthesis of IgM in unstimulated cultures and of both IgM and IgG in cultures stimulated with pokeweed mitogen was markedly reduced in patients with Hodgkin's disease, whereas the mean level of the spontaneous IgG synthesis was enhanced. The degree and frequency of in vitro abnormalities were not influenced by disease stage or histology.Depression of pokeweed mitogen-induced immunoglobulin synthesis did not correlate with excessive number of monocytes and it was unaffected by removal of phagocytic cells or addition to the cultures of monocytes from normal individuals. On the other hand, monocytes isolated from blood of patients with Hodgkin's disease were even more effective than normal monocytes in supporting pokeweed mitogen-induced immunoglobulin synthesis by normal phagocyte-depleted mononuclear cells. Synthesis of both IgM and IgG induced by pokeweed mitogen remained subnormal after addition to patient B cell cultures of autologous irradiated T cells or allogeneic normal T lymphocytes. T cells from patients with Hodgkin's disease appeared at least as effective as normal T cells in helping pokeweed mitogen-induced immunoglobulin production by normal B cells. However, when normal T cells were co-cultured with B cells from patients with Hodgkin's disease, spontaneous IgG synthesis declined, whereas the addition of patient T cells

Defective in vitro production of gamma-interferon and tumor necrosis factor-alpha by circulating T cells from patients with the hyper-immunoglobulin E syndrome.

“…The IgE synthesis in vitro induced by TCC was calculated by subtracting values of both preformed and spontaneously synthesized IgE (when detectable). The RIA used for detecting IgG in culture supernatants has also been described (6). Values of TCC-induced IgG higher than 1 gg/ml and ofTCC-induced IgE synthesis higher than 0.5 ng/ml above background levels (preformed plus spontaneous) were considered as positive.…”

In vitro IgE Synthesis Induced by Human T Cell Clones in Normal B Cells and Its Suppression by Heterogenous T Cell Populations

“…In some experiments, IgE concen trations were measured in culture SN using both the in-house and Rast-Pharmacia l25I-labelled anti-IgE reagents. The RIA used for detecting IgM and IgG in culture SN has been described previously in detail [14], …”

Helper activity for immunoglobulin synthesis of T helper type 1 (Th1) and Th2 human T cell clones: the help of Th1 clones is limited by their cytolytic capacity.

“…After 10 d, culture SN were collected and assayed for their Ig content. The immunoradiometric assay used for detecting IgM, IgG, and IgA has been described previously in detail (12). The procedure for the measurement of IgE has also been described in detail elsewhere (13).…”