In vitro IgE Synthesis Induced by Human T Cell Clones in Normal B Cells and Its Suppression by Heterogenous T Cell Populations

Romagnani, S; Prete, G.; Maggi, Enrico; Ricci, M

doi:10.1159/000234241

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…3 , unfractionated T cells inhibited in a dose-dependent mannner the IgE synthesis induced by T C C on autologous B cells. In contrast, the same cells had no detectable inhibitory effect on the TCC-induced IgE synthesis, thus supporting the view that also in humans, as in rodents, IgE-producing cells are exquisitely sensitive to suppressor cell activity (12,13).…”

Section: Supp~esso~ T Cells Und Ise Synthesis In Mansupporting

confidence: 59%

Development and application of in vitro models for the study of human IgE synthesis

Romagnani

Prete

Maggi

1988

Allergy

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Section: Supp~esso~ T Cells Und Ise Synthesis In Mansupporting

confidence: 59%

Development and application of in vitro models for the study of human IgE synthesis

Romagnani

Prete

Maggi

1988

Allergy

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“…Anti-CD3 antibodies were shown to stimulate IgE production by human B cells when T cell clones were used [7,, but on unseparated T cells CD3 stimulation proved to be inhibitory for IgE production [5,7,23,261. This suggests a lack of suppressor cell activity when T cell clones are used; however, when anti-CD3 is used with unseparated Tcells at high cell concentrations it causes an inhibition of IgE production probably via the same mechanism as we have described in this report.There is one report describing positive effects of anti-CD3 stimulation on IgE production by PBL cultured at high cell densities (1 x 106/ml),without the addition of IL4, but the IgE levels that were obtained were very low [27].…”

Section: Discussionmentioning

confidence: 99%

Human transferrin allows efficient IgE production by anti‐CD3‐stimulated human lymphocytes at low cell densities

Pouw-Kraan

Kooten

Oers³

et al. 1991

Eur J Immunol

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A solid-phase-coupled anti-CD3 T cell activation system was used to study the regulation of human IgE production in vitro. Using 5000 peripheral blood lymphocytes from healthy donors, containing 10%-20% B lymphocytes and no monocytes. IgE was produced very efficiently on a per cell basis. A key observation was that apart from interleukin (IL) 4, human transferrin was essential for IgE production. Furthermore it was found that IgE was produced at low densities only; at higher cell concentrations IgE production was completely abrogated, whereas IgM production increased with increasing cell density. This inhibition at higher cell densities is probably mediated by IL2. Addition of low amounts (6 U/ml) of IL2 strongly enhanced IgE and IgM production at low cell densities, but higher concentrations of IL2 (50 U/ml) were strongly inhibitory for IgE production.

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Cytokines Involved in the Pathophysiology of IgE Response

Prete

1992

Int J Immunopathol Pharmacol

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Recent data in both mouse and human systems have clearly indicated that IL-4 plays an essential role in the induction of IgE response, whereas IFN-γ exerts an opposite effect on this phenomenon. However, IL-4 alone is unable to induce IgE synthesis. A prior membrane contact signal delivered by activated T cells is required by B cells to synthesize IgE in response to IL-4. A cognate interaction between B and T cells producing IL-4 (but not IFN-γ) is optimal for induction of IgE synthesis. However, when such IL-4 producing T cells are activated, they may provide B cells with a membrane signal, through a non-cognate interaction, which also results in induction of IgE synthesis. Other cytokines, such as IL-2 and IL-6, play an auxiliary role in IL-4 dependent IgE production. Analyses at the clonal level of the profile of cytokine production by helper T cells have shown that imbalances between IL-4 and IFN-γ producing T cells can be detected in patients with hyperproduction of IgE. In children with hyper-IgE syndrome a strong reduction of circulating T cells able to produce IFN-γ (and TNF-α) was found, whereas both, reduction of IFN-γ-producing and increase of IL-4 producing T cells were found in patients with parasitic infestation. In allergic inflammatory infiltrates, such as those found in vernal conjuctivitis, helper T cells able to produce IL-4 and to induce IgE synthesis accounted for the large majority of infiltrating T cells. It is reasonable to suggest that systemic or microenvironmental alterations of the balance between IL-4- and IFN-γ-producing T cells may be involved, at least in part, in the pathophysiology of the increased igE response occurring in a number of human diseases.

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In vitro IgE Synthesis Induced by Human T Cell Clones in Normal B Cells and Its Suppression by Heterogenous T Cell Populations

Cited by 3 publications

References 7 publications

Development and application of in vitro models for the study of human IgE synthesis

Development and application of in vitro models for the study of human IgE synthesis

Human transferrin allows efficient IgE production by anti‐CD3‐stimulated human lymphocytes at low cell densities

Cytokines Involved in the Pathophysiology of IgE Response

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