Ability of dairy strains of lactic acid bacteria to bind a common food carcinogen, aflatoxin B1

Ability of a Lactobacillus rhamnosus strain cultured in milk whey based medium to bind aflatoxin B1

“…The cell wall of microorganisms is mainly composed by polysaccharides, proteins, and lipids, which contain abundant functional groups such as carboxyl, hydroxyl, phosphate, and amine and hydrophobic adsorption sites, such as aliphatic carbon chains and aromatic rings. Haskard et al (2001) and El-Nezami et al (1998) analyzed viable cells of L. rhamnosus and obtained about 80% of AFB 1 binding from a contaminated medium. Rahaie et al (2012) observed that heat treated cells of L. rhamnosus GG could adsorb 85% of AFB 1 (10 ng/ml) from the medium.…”

“…The AFB 1 binding assays were conducted using the method described by El-Nezami et al (1998) with some adaptations. Aliquots of the culture broth containing 1 × 10 10 cells were transferred to test tubes and centrifuged (CT-14 000 -Cientec, Piracicaba, SP, Brazil) at 1800 × g for disposal as waste poses serious pollution problems for the surrounding environment (Panesar et al, 2007).…”

“…Eighteen different types of aflatoxins have been identified so far, and aflatoxin B 1 (AFB 1 ) is the most prevalent and toxic metabolite produced by the fungi (Bhat et al, 2010). Several studies have demonstrated the efficiency of different species and strains of LAB in adsorbing aflatoxins from contaminated media (El-Nezami et al, 1998;Pierides et al, 2000;Oatley et al, 2000;Haskard et al, 2001;Peltonen et al, 2001;Azab et al, 2005;Fazeli et al, 2009;Bovo et al, 2013).…”

Biological Control of Molds and Mycotoxins in Foods

“…El-Nezami et al (58) reported the ability of lactic acid bacteria to remove aflatoxin B 1 from artificially contaminated liquid media. The removal was strain dependent and very fast, with two strains of L. rhamnosus removing about 80% of the toxin at the beginning of the incubation time.…”

“…The ability of lactic acid bacteria to remove toxins in vitro and in vivo was compared by El-Nezami et al (58,62). In in vitro experiments, L. rhamnosus was able to remove approximately 80% of the initial amount of aflatoxin B 1 .…”