AA amyloidosis in Chinese Shar-pei dogs: Immunohistochemical and amino acid sequence analyses

Johnson, Kenneth H.; Sletten, K; Hayden, D. W.; O’Brien, Timothy; Rossow, Kurt; Westermark, Per

doi:10.3109/13506129509031893

Cited by 13 publications

(11 citation statements)

References 19 publications

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“…Endogenous peroxidase was quenched with 3% hydrogen peroxide. Tissues were then blocked, sequentially incubated in polyclonal rabbit anti-canine AA antibody, 54,18 biotinylated goat anti-rabbit secondary, and streptavidin horse-radish peroxidase, and then visualized using a 3,3′-diaminobenzidine substrate. Positive controls included a Shar Pei dog kidney with amyloid and an island fox kidney with AA amyloid confirmed by mass spectrometry.…”

Section: Methodsmentioning

confidence: 99%

Systemic Amyloid A Amyloidosis in Island Foxes (Urocyon littoralis)

et al. 2015

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Systemic amyloid A (AA) amyloidosis is highly prevalent (34%) in endangered island foxes (Urocyon littoralis) and poses a risk to species recovery. Although elevated serum amyloid A from prolonged or recurrent inflammation predisposes to AA amyloidosis, additional risk factors are poorly understood. Here we define the severity of glomerular and medullary renal amyloid and identify risk factors for AA amyloidosis in 321 island foxes necropsied from 1987 through 2010. In affected kidneys, amyloid more commonly accumulated in the medullary interstitium than in the glomeruli [98% (78/80) versus 56% (45/80), respectively, p < 0.0001], and medullary deposition was more commonly severe [19% (20/105)] as compared to glomeruli [7% (7/105), p = 0.01]. Univariate odds ratios (ORs) of severe renal AA amyloidosis were greater for short- and long-term captive foxes compared to free-ranging (OR=3.2, 3.7, respectively, overall p = 0.05) and females compared to males (OR = 2.9, p = 0.05). Multivariable logistic regression revealed independent risk factors for amyloid development were increasing age class (OR = 3.8, p < 0.0001), San Clemente Island subspecies compared to San Nicolas Island subspecies (OR = 5.3, p = 0.0003), captivity (OR = 5.1, p = 0.0001), and nephritis (OR = 2.3, p = 0.01). The increased risk associated with the San Clemente subspecies or captivity suggests roles for genetic as well as exogenous risk factors in the development of AA amyloidosis.

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Section: Methodsmentioning

confidence: 99%

Systemic Amyloid A Amyloidosis in Island Foxes (Urocyon littoralis)

et al. 2015

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“…Nonspecific binding was blocked with normal goat serum (1 : 10 in Tris-buffered saline) for 15 minutes followed by incubation overnight at 4uC with primary antibody (rabbit anti-dog AA at 1/400 dilution). 12 After rinsing, sections were incubated with biotinylated goat anti-rabbit (cat. No.…”

Section: Methodsmentioning

confidence: 99%

“…In animals, familial amyloidosis has been best described in Shar-Pei dogs 6,12 and Abyssinian 2 and Siamese 26 cats. In both the Abyssinian and Siamese cats, there are specific amino acid sequence variations in the SAA protein that may render the protein more amyloidogenic, but additional factors affecting production or processing of the precursor protein may also be involved in these familial AA amyloidoses.…”

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confidence: 99%

Amyloidosis in Black-footed Cats (Felis nigripes)

et al. 2008

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Abstract. A high prevalence of systemic amyloidosis was documented in the black-footed cat (Felis nigripes) based on a retrospective review of necropsy tissues (n 5 38) submitted as part of ongoing disease surveillance. Some degree of amyloid deposition was present in 33 of 38 (87%) of the examined cats, and amyloidosis was the most common cause of death (26/38, 68%). Amyloid deposition was most severe in the renal medullary interstitium (30/33, 91%) and glomeruli (21/33, 63%). Other common sites included the splenic follicular germinal centers (26/31, 84%), gastric lamina propria (9/23, 39%), and intestinal lamina propria (3/23, 13%). Amyloid in all sites stained with Congo red, and in 13 of 15 (87%) cats, deposits had strong immunoreactivity for canine AA protein by immunohistochemistry. There was no association with concurrent chronic inflammatory conditions (P 5 .51), suggesting that amyloidosis was not secondary to inflammation. Adrenal cortical hyperplasia, a morphologic indicator of stress that can predispose to amyloid deposition, was similarly not associated (P 5 .09) with amyloidosis. However, adrenals were not available from the majority of cats without amyloidosis; therefore, further analysis of this risk factor is warranted. Heritability estimation suggested that amyloidosis might be familial in this species. Additionally, tissues from a single free-ranging black-footed cat had small amounts of amyloid deposition, suggesting that there could be a predilection for amyloidosis in this species. Research to identify the protein sequence of serum amyloid A (SAA) in the black-footed cat is needed to further investigate the possibility of an amyloidogenic SAA in this species.

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“…However, the literature data indicate that amyloidosis in Shar-Pei dogs has an interesting distinctive feature of prevailing medullar interstitial deposition of amyloid, whereas glomerular amyloidosis is common in other breeds of dogs. [7,11] Initial amyloid deposits have almost no clinical manifestations; therefore, the amyloidosis diagnostics in Shar-Pei dogs is extremely difficult at this stage. The clinical picture of renal pathology acquires distinctive features at later stages, with the involvement of the glomeruli and total substitution of the renal tissue with amyloid.…”

Section: Resultsmentioning

confidence: 99%

“…[16] SAA production in liver depends on a range of cytokines, including IL-1, IL-6, tumor necrosis factor. [4,11] The formation of the AA-amyloid fibrils is divided into two stages. The first stage includes synthesis of the precursor protein in the amounts, sufficient for the amyloid deposition.…”

Section: Resultsmentioning

confidence: 99%

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Sotnikova

2017

AJP

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Introduction: Familial Shar-Pei amyloidosis is an autoinflammatory systemic disease characterized by pathological synthesis of the fibrillary protein in the cells of the reticuloendothelial system, followed by amyloid formation. The purpose of our research is mainly focused on the investigation of specific structural histological changes in kidneys, liver, and spleen of the Shar-Pei dogs suffering from familial amyloidosis. Materials and Methods: The studies included autopsy and post-mortem examination of the Shar-Pei dogs with the presumptive diagnosis of familial amyloidosis or other diagnoses. Samples of kidney, liver, and spleen tissues of all cadavers were collected for histological examination. Results: Our studies showed that amyloid was formed within the ground substance of the connective tissue. Early amyloid deposits were observed in the spleen samples, providing the pathomorphological marker of the initial stage of the process generalization, whereas during the later stages, amyloid was found in kidneys, liver, and myocardium. Gradually increasing amyloid deposits lead to compression and atrophy of the parenchymal cells, sclerosis, and multiple organ dysfunction syndrome, manifesting as a wide range of clinical signs. Discussion: As a result of the conducted post-mortem examination, we have revealed systemic amyloidosis in the cadavers of the animals, initially admitted with various pathologies, which proves the importance and relevance of timely diagnostics, detection of clinical manifestations, and latent forms of the condition. Histological examination is one of the most accurate diagnostic methods for this pathology.

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AA amyloidosis in Chinese Shar-pei dogs: Immunohistochemical and amino acid sequence analyses

Cited by 13 publications

References 19 publications

Systemic Amyloid A Amyloidosis in Island Foxes (Urocyon littoralis)

Systemic Amyloid A Amyloidosis in Island Foxes (Urocyon littoralis)

Amyloidosis in Black-footed Cats (Felis nigripes)

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