A Water Extract ofArtemisia capillarisPrevents 2,2'-Azobis(2-Amidinopropane) Dihydrochloride-Induced Liver Damage in Rats

Abstract

A water extract of Artemisia capillaris Thunberg (Compositae) was investigated for protective effects against oxidative stress induced by 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) in Sprague-Dawley male rats. Rats were orally administered A. capillaris water extract (ACWE; 7.5 g/kg) for 7 days before AAPH treatment (60 mg/kg). AAPH intoxication significantly elevated enzyme markers of liver injury (glutamic oxaloacetic transaminase and glutamic pyruvic transaminase). The pre-administration of ACWE significantly reduced the liver-damaging effects of AAPH as indicated by the low levels of these enzymes. Moreover, the ACWE administration significantly attenuated the accumulation of thiobarbituric acid-reactive substances in both plasma and liver tissues compared with those of rats administered AAPH alone. Furthermore, ACWE administration slightly improved the liver reduced glutathione levels and enhanced the production of antioxidant enzymes like catalase. A. capillaris contained 10.1 mg of catechin in 100 g of dried sample; the high-performance liquid chromatography results showed catechin composition in the ACWE to be 28% (-)-epigallocatechin gallate, 49% (-)- epigallocatechin, and 23% other catechins. These observations clearly indicate that ACWE contains antioxidant catechins capable of ameliorating the AAPH-induced hepatic injury by virtue of its antioxidant activity.

Isorhamnetin-3-O-galactoside Protects against CCl4-Induced Hepatic Injury in Mice

“…An aqueous extract of Artemisia capillaris was shown to inhibit interleukin (IL)-1 receptor- and tumor necrosis factor (TNF) receptor-induced cytotoxicity and ethanol-induced apoptosis of HepG2 cells (Koo et al ., 2002). A previous study reported that Artemisia capillaris reduced the lipopolysaccharide-induced inflammatory response in a human hepatoma cell line and in the rat liver (Hong et al ., 2004), and prevented 2,2'-azobis(2-amidinopropane) dihydrochloride-induced liver damage in rats (Han et al ., 2006).…”

Composition variation in essential oils of Artemisia nilagirica and Artemisia capillaris, growing in India

“…The methanolic extract of aerial part from A. capillaris showed antimutagenic activity (Park et al, 1996). A. capillaris has also been reported for cytoprotective and antioxidant activity (Hong et al, 2007;Seo et al, 2003 inflammatory and diuretic purposes and in the treatment of jaundice (Tang and Eisenbrand, 1992;Han et al, 2006;Han et al, 2005;Jang et al, 2005;Hong et al, 2004 andHu et al, 2000). A group of coumarins and flavonol glycosides have also been reported from the inflorescence of A. capillaris (Yamahara et al, 1989 andFakeya et al, 1976).…”

Inchingorei-san (TJ-117) and Artemisiae Capillaris Herba Induced Prolonged Survival of Fully Mismatched Cardiac Allografts and Generated Regulatory Cells in Mice

“…ACH, one component of TJ-117, has been widely used as a liver protective agent, diuretic, analgesic, lipid digestive agent, antimicrobial agent [39], and a remedy for the treatment of skin inflammatory disorders [40]. The several pharmacological actions of ACH include antiobesity action [41] and liver protective action mediated by antioxidants [42, 43]. In this study, allografts obtained 30 days after transplantation from mice given ACH had only a few myocardial injuries with infiltrating leukocytes and mild obliterative vasculopathy, whereas allografts from untreated group had severe myocardial injuries and obliterative vasculopathy as shown in our histologic examinations (Figure 1(c)).…”