A viscosity-dependent affinity sensor for continuous monitoring of glucose in biological fluids

Boss, Christophe; Meurville, Eric; Sallèse, Jean-Michel; Ryser, Peter

doi:10.1016/j.bios.2011.09.016

Cited by 29 publications

(21 citation statements)

References 21 publications

(22 reference statements)

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“…MEMS cantilever based viscosity sensors have been previously designed specifically for glucose concentration detection [23][24][25][26]. Glucose levels are detected based on affinity binding of glucose sensitive polymeric matrix encapsulated over the sensor.…”

Section: Introductionmentioning

confidence: 99%

Microcantilever based disposable viscosity sensor for serum and blood plasma measurements

Çakmak

Elbüken

Ermek

et al. 2013

Methods

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a b s t r a c tThis paper proposes a novel method for measuring blood plasma and serum viscosity with a microcantilever-based MEMS sensor. MEMS cantilevers are made of electroplated nickel and actuated remotely with magnetic field using an electro-coil. Real-time monitoring of cantilever resonant frequency is performed remotely using diffraction gratings fabricated at the tip of the dynamic cantilevers. Only few nanometer cantilever deflection is sufficient due to interferometric sensitivity of the readout. The resonant frequency of the cantilever is tracked with a phase lock loop (PLL) control circuit. The viscosities of liquid samples are obtained through the measurement of the cantilever's frequency change with respect to a reference measurement taken within a liquid of known viscosity. We performed measurements with glycerol solutions at different temperatures and validated the repeatability of the system by comparing with a reference commercial viscometer. Experimental results are compared with the theoretical predictions based on Sader's theory and agreed reasonably well. Afterwards viscosities of different Fetal Bovine Serum and Bovine Serum Albumin mixtures are measured both at 23°C and 37°C, body temperature. Finally the viscosities of human blood plasma samples taken from healthy donors are measured. The proposed method is capable of measuring viscosities from 0.86 cP to 3.02 cP, which covers human blood plasma viscosity range, with a resolution better than 0.04 cP. The sample volume requirement is less than 150 ll and can be reduced significantly with optimized cartridge design. Both the actuation and sensing are carried out remotely, which allows for disposable sensor cartridges.

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Section: Introductionmentioning

confidence: 99%

Microcantilever based disposable viscosity sensor for serum and blood plasma measurements

Çakmak

Elbüken

Ermek

et al. 2013

Methods

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“…Resonating cantilevers and plates have been the most frequently used type of MEMS viscometers [16][17][18] which are usually actuated by means of piezoelectric material [19][20][21], magnetic field [22][23][24] or alternating electricity current [25][26][27]. Theoretical studies and models connect variations in fluid density and viscosity to the shift in resonant frequency and quality factor, respectively [28][29][30].…”

Section: Vibrating Microcantilevermentioning

confidence: 99%

3D Suspended Polymeric Microfluidics (SPMF3) with Flow Orthogonal to Bending (FOB) for Fluid Analysis through Kinematic Viscosity

2017

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Abstract:Measuring of fluid properties such as dynamic viscosity and density has tremendous potential for various applications from physical to biological to chemical sensing. However, it is almost impossible to affect only one of these properties, as dynamic viscosity and density are coupled. Hence, this paper proposes kinematic viscosity as a comprehensive parameter which can be used to study the effect of fluid properties applicable to various fluids from Newtonian fluids, such as water, to non-Newtonian fluids, such as blood. This paper also proposes an ideal microplatform, namely polymeric suspended microfluidics (SPMF 3 ), with flow plane orthogonal to the bending plane of the structure, along with tested results of various fluids covering a wide range of engineering applications. Kinematic viscosity, also called momentum diffusivity, considers changes in both fluid intermolecular forces and molecular inertia that define dynamic viscosity and fluid density, respectively. In this study a 3D suspended polymeric microfluidic system (SPMF 3 ) was employed to detect changes in fluid parameters such as dynamic viscosity and density during fluid processes. Using this innovative design along with theoretical and experimental results, it is shown that, in fluids, the variations of fluid density and dynamic viscosity are not easily comprehensible due to their interconnectivity. Since any change in a fluid will affect both density and dynamic viscosity, measuring both of them is necessary to identify the fluid or process status. Finally, changes in fluid properties were analyzed using simulation and experiments. The experimental results with salt-DI water solution and milk with different fat concentrations as a colloidal fluid show that kinematic viscosity is a comprehensive parameter that can identify the fluids in a unique way using the proposed microplatform.

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“…To evaluate the long term membrane functionality, a sensor was fabricated as described in Boss et al [12]. Basically, the sensor includes both an actuating and a sensing piezoelectric diaphragms as well as a flow-resistive microchannel used for viscosity detection.…”

Section: Nanoporous Membrane In a Glucose Sensormentioning

confidence: 99%

“…Amongst others, one approach has been the affinity sensing technique, which is based on the binding of glucose with a carbohydrate-specific molecule [10,11]. We recently reported promising results for an affinity based sensor relying on the detection of the viscosity variation of a sensitive fluid with the glucose concentration [12]. The sensitive fluid principle relies on the competitive binding of glucose and dextran with a glucose-specific binding protein, Concanavalin A (ConA).…”

Section: Introductionmentioning

confidence: 99%

Size-selective diffusion in nanoporous alumina membranes for a glucose affinity sensor

Boss

Meurville

Sallèse

et al. 2012

Journal of Membrane Science

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a b s t r a c tThe retention, diffusion and structural properties of 2-4 nm nanoporous alumina membranes were investigated in view of their integration as size-selective interface in a glucose sensor. These membranes exhibit remarkable glucose diffusion properties with only a 5-fold reduction compared to free diffusion in water. The retention of the glucose-binding protein (Concanavalin A), which is characterized by a hydrodynamic radius of only 3.3 nm, was almost complete during at least 35 days. This high selectivity was also confirmed by SEM picture analysis showing a highly uniform pore size distribution. Finally, the glucose sensor including a nanoporous membrane as size-selective interface was able to measure glucose levels in physiological solution during 25 days, which confirms that annealed alumina membranes are well suited for size-selective interface of biosensors.

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A viscosity-dependent affinity sensor for continuous monitoring of glucose in biological fluids

Cited by 29 publications

References 21 publications

Microcantilever based disposable viscosity sensor for serum and blood plasma measurements

Microcantilever based disposable viscosity sensor for serum and blood plasma measurements

3D Suspended Polymeric Microfluidics (SPMF3) with Flow Orthogonal to Bending (FOB) for Fluid Analysis through Kinematic Viscosity

Size-selective diffusion in nanoporous alumina membranes for a glucose affinity sensor

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