Size-selective diffusion in nanoporous alumina membranes for a glucose affinity sensor

Abstract

a b s t r a c tThe retention, diffusion and structural properties of 2-4 nm nanoporous alumina membranes were investigated in view of their integration as size-selective interface in a glucose sensor. These membranes exhibit remarkable glucose diffusion properties with only a 5-fold reduction compared to free diffusion in water. The retention of the glucose-binding protein (Concanavalin A), which is characterized by a hydrodynamic radius of only 3.3 nm, was almost complete during at least 35 days. This high selectivity was also confirmed by SEM picture analysis showing a highly uniform pore size distribution. Finally, the glucose sensor including a nanoporous membrane as size-selective interface was able to measure glucose levels in physiological solution during 25 days, which confirms that annealed alumina membranes are well suited for size-selective interface of biosensors.

Tailored fibro-porous structure of electrospun polyurethane membranes, their size-dependent properties and trans-membrane glucose diffusion

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Impact of ConcanavalinA affinity in the intracellular fate of Protein Corona on Glucosamine Au nanoparticles

“…The hydrodynamic radius reported in literature for BSA in around 3.4–3.5 nm 44 . The hydrodynamic radius of Con A in its dimeric form is 3.3 nm; in the quaternary form is 4.4 nm 45 . The NPs d H does not increase after corona formation as expected.…”

Electrospun polyurethane-core and gelatin-shell coaxial fibre coatings for miniature implantable biosensors

“…Based on the assumptions, combining the diffusion equation with Fick’s law and mass balance conditions between the two chambers A and B given Eq. 5 [16, 17]: $$\begin{array}{c}left({\mathrm{normalC}}_{\mathrm{normalB}}-{\mathrm{normalC}}_{\mathrm{normalA}})/({\mathrm{normalC}}_{\mathrm{B}0}-{\mathrm{normalC}}_{\mathrm{A}0})={\mathrm{normale}}^{-(\mathrm{t}/\mathrm{\tau })},\text{with}\\ \mathrm{\tau }=[\mathrm{d}/({\mathrm{normalD}}_{\text{eff}}\mathrm{S})]\times [({\mathrm{normalV}}_{\mathrm{normalA}}{\mathrm{normalV}}_{\mathrm{normalB}})/({\mathrm{normalV}}_{\mathrm{normalA}}+{\mathrm{normalV}}_{\mathrm{normalB}})]\end{array}$$ where C A , C B and V A , V B are the concentration and volume of the chamber A and B, d is the membrane thickness, S is the membrane surface area, t is time and D eff is the effective diffusion constant for the membrane.…”