A Validated Voltammetric Procedure for Quantification of the Antifungal Drug Griseofulvin in Bulk Form, Tablets, and Biological Fluids at a Mercury Electrode

Abstract

Griseofulvin is an antifungal antibiotic used to treat various pathogenic mycotic diseases. The voltammetric behavior of griseofulvin at a hanging mercury drop electrode in Britton-Robinson buffers of pH 2 -11.5 was studied and discussed. A fully validated sensitive square-wave adsorptive cathodic stripping voltammetric procedure was described for direct determination of bulk griseofulvin substance. The procedure was based on the reduction of the .C5 5O double bond of griseofulvin molecule following its preconcentration onto a hanging mercury drop electrode in a Britton-Robinson buffer of pH 10. Limits of detection (LOD) and quantitation (LOQ) of 5.8 Â 10 210 M and 1.93 Â 10 29 M bulk griseofulvin were achieved, respectively. The proposed stripping voltammetric procedure was successfully applied to assay griseofulvin in tablets and in spiked human serum and urine samples. LOD of 8.65 Â 10 210 M and 6.6 Â 10 29 M and LOQ of 2.88 Â 10 29 M and 2.2 Â 10 28 M griseofulvin in spiked human serum and urine samples, respectively, were achieved.

Two Spectrophotometric Methods for the Determination of Griseofulvin in Tablets

“…Its formula is C17H17ClO6 and its molecular mass: 352.766 g/mol. It has the structure shown in Figure ( Various analytical techniques for the determination of griseofulvin in pure form, in different chemical mixtures, in biological fluids and in fermentation samples were described including the use of paper chromatography, thin layer chromatography, gas chromatography, high performance liquid chromatography [2][3][4][5][6][7][8], electrometric method [9], and spectrophotometry, spectrofluorimetry [10][11][12][13][14][15][16] microbiological assay for the estimation of griseofulvin.…”