A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption?

Jafarian, Vahab; Ghaffari, F.

doi:10.1134/s0026261717010064

Cited by 32 publications

(9 citation statements)

References 40 publications

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“…Moreover, cadmium accumulation in transgenic bacteria increased with incubation time, and the cadmium content in cells incubated for 16 h (54.0 ng/10 7 × cfu) was 5.3 times higher than that of cells incubated for 4 h (10.2 ng/10 7 × cfu) (Figure d). The results showed that cadmium was removed from the culture by intracellular biosorption . Our result was also consistent with that of Chen et al for Pb accumulation in flagellin-recombinant bacteria …”

Section: Resultssupporting

confidence: 93%

Remediation and Mechanisms of Cadmium Biosorption by a Cadmium-Binding Protein from Lentinula edodes

Dong

Huang

Bian

et al. 2019

J. Agric. Food Chem.

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Cadmium bioremediation with metal-binding proteins is primarily conducted using metallothioneins (MTs). However, in the present study, we investigated a non-MT cadmium-binding protein from Lentinula edodes (LECBP) as a remediation tool for cadmium biosorption in Escherichia coli. The results indicated that the expression of LECBP significantly enhanced the cadmium biosorption capacity of transgenic E. coli. The secondary structure and conformation of LECBP were changed after binding with cadmium as evidenced by circular dichroism and fluorescence spectroscopy. The results of Fourier transform infrared spectroscopy indicated that carboxyl oxygen and amino nitrogen atoms were involved in the interaction between LECBP and cadmium. The results further demonstrated that glutamic acid and histidine residues are the potential binding sites. Our results have thus provided new insights into cadmium bioremediation in an aquatic environment.

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Section: Resultssupporting

confidence: 93%

Remediation and Mechanisms of Cadmium Biosorption by a Cadmium-Binding Protein from Lentinula edodes

Dong

Huang

Bian

et al. 2019

J. Agric. Food Chem.

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“…In the present study, L. adecarboxylata MO1 inoculation significantly protected the plants from the inhibitory effects of higher concentrations of Zn by reducing the Zn content (Kang et al 2017). The reduction of Zn content at higher Zn toxicity levels by this bacterium might be due to Zn removal through adsorption and desorption mechanisms, as well as solubilization and leaching mechanisms of the bacterium (Pirhadi et al 2016;Jafarian and Ghaffari 2017). Enhanced Zn uptake triggered Fe deficiency (Lešková et al 2017) that reduced root and shoot growth and chlorophyll content as well as chlorosis in young leaves (Marschner 2012;Lešková et al 2017).…”

Section: Discussionsupporting

confidence: 46%

Ameliorative effect of indole-3-acetic acid- and siderophore-producing Leclercia adecarboxylata MO1 on cucumber plants under zinc stress

Kang

Shahzad

Khan

et al. 2021

Journal of Plant Interactions

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This study investigated the plant growth-promoting effects of Leclercia adecarboxylata MO1 for Zn stress mitigation and plant growth improvement in Zn-contaminated soil. Results demonstrated that L. adecarboxylata MO1 produced siderophores that could solubilize Zn and silicate, had a tolerance to elevated levels of Zn supplementation (2 and 5 mM) in growth mediums, and produced significant amounts of indole-3-acetic acid (IAA). It was also found to promote plant growth under both control conditions and Zn toxicity (2 and 5 mM). Furthermore, L. adecarboxylata MO1 positively regulated physiochemical attributes by decreasing hydrogen peroxide (H 2 O 2) and Zn uptake in both roots and shoots, improving antioxidant systems (e.g. catalase (CAT), peroxidase (POD), polyphenol peroxidase (PPO), superoxide anion (SOA), lipid peroxidation (MDA), and glutathione (GSH)), and reducing stress-responsive endogenous abscisic acid (ABA) and salicylic acid (SA) in plants grown under Zn toxicity of 2 and 5 mM, compared with non-inoculated plants.

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“…Later, other works have also shown that the expression of MT enhanced binding affinity and cellular tolerance in the same host (Sihai et al, 2008;He et al, 2011). This is also the case when expressed in E. coli (Singh et al, 2008;Jafarian and Ghaffari, 2017;Shahpiri and Mohammadzadeh, 2018). Furthermore, overexpression of metal-tolerant proteins, CrMTP4, has shown to increase Cd tolerance in C. reinhardtii by 2.29 folds and resulted in a 3.09fold increase in Cd bioaccumulation (Ibuot et al, 2017).…”

Section: Synthetic Biology Workflow-designmentioning

confidence: 82%

Synthetic Biology-Based Approaches for Microalgal Bio-Removal of Heavy Metals From Wastewater Effluents

Sattayawat

Yunus

Noirungsee

et al. 2021

Front. Environ. Sci.

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Heavy metal polluted wastewater from industries is currently one of the major environmental concerns leading to insufficient supply of clean water. Several strategies have been implemented to overcome this challenge including the use of microalgae as heavy metal bio-removers. However, there are still limitations that prevent microalgae to function optimally. Synthetic biology is a new biological discipline developed to solve challenging problems via bioengineering approaches. To date, synthetic biology has no universally affirmed definitions; however, it is uncontroversial that synthetic biology utilizes a constructive library of genetic standardized parts to create new biological systems or to redesign existing ones with improved characteristics. In this mini-review, we present state-of-the-art synthetic biology-based approaches that can be used to enhance heavy metal bio-removal from wastewater effluents by microalgae with a narrative synthetic biology workflow (Design-Build-Test-Learn cycle) to guide future developments of more advanced systems. We also provide insights into potent genes and proteins responsible for the bio-removal processes for stepwise developments of more advanced systems. A total of 49 unique genes and proteins are listed based on their eight heavy metals (Mn, Fe, Cu, Zn, As, Cd, Hg, and Pb) bio-removal functions in transport system, cellular tolerance, synthesis of key players in heavy metal bio-removal, biotransformation of heavy metals, and gene expression regulation. Thus, with our library, genetic parts are ready to be recruited for any synthetic biology-based designs. Thereby, this mini-review identifies potential avenues of future research and maps opportunities to unleash more potential of microalgae as heavy metal bio-removers with synthetic biology.

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A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption?

Cited by 32 publications

References 40 publications

Remediation and Mechanisms of Cadmium Biosorption by a Cadmium-Binding Protein from Lentinula edodes

Remediation and Mechanisms of Cadmium Biosorption by a Cadmium-Binding Protein from Lentinula edodes

Ameliorative effect of indole-3-acetic acid- and siderophore-producing Leclercia adecarboxylata MO1 on cucumber plants under zinc stress

Synthetic Biology-Based Approaches for Microalgal Bio-Removal of Heavy Metals From Wastewater Effluents

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