A Unique<i>Mycobacterium</i>Species Isolated from an Epizootic of Striped Bass (<i>Morone saxatilis</i>)

Rhodes, Martha W.; Kator, Howard; Kotob, Shaban; Berkum, Peter van; Kaattari, Ilsa; Vogelbein, Wolfgang K.; Floyd, Margaret M.; Butler, W. Ray; Quinn, Frederick D.; Ottinger, Christopher A.; Shotts, Emmett B.

doi:10.3201/eid0705.017523

Cited by 51 publications

(49 citation statements)

References 21 publications

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“…In contrast to the ulcerative skin lesions documented to occur in zebrafish and striped bass (Morone saxatilis) infected with Mycobacterium spp., neither inflammatory lesions nor any gross signs of inflammation were observed in medaka, at any time during infection (Fig. 2) (Rhodes et. al., 2001;Van der Sar et.…”

Section: Marinum Establishes An Acute or Chronic Infection In Medamentioning

confidence: 97%

Mycobacterium marinum produces long-term chronic infections in medaka: A new animal model for studying human tuberculosis

Broussard¹,

Ennis²

2007

Comparative Biochemistry and Physiology Part C: Toxicology &

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Human infection by Mycobacterium tuberculosis is endemic, with approximately 2 billion infected and is the most common cause of adult death due to an infectious agent. Because of the slow growth rate of M. tuberculosis and risk to researchers, other species of Mycobacterium have been employed as alternative model systems to study human tuberculosis (TB). Mycobacterium marinum may be a good surrogate pathogen, conferring TB-like chronic infections in some fish. Medaka (Oryzias latipes) has been established for over five decades as a laboratory fish model for: toxicology, genotoxicity, teratogenesis, carcinogenesis, classical genetics and embryology. We are investigating if medaka might also serve as a host for M. marinum in order to model human TB. We show that both acute and chronic infections are inducible in a dose dependent manner. Colonization of target organs and systemic granuloma formation has been demonstrated through the use of histology. M. marinum expressing green fluorescent protein (Gfp) was used to monitor bacterial colonization of these organs in fresh tissues as well as in intact animals. Moreover, we have employed the SeeThrough fish line, a variety of medaka devoid of major pigments, to monitor real-time disease progression, in living animals. We have also compared the susceptibility of another prominent fish model, zebrafish (Danio rerio), to our medaka-M. marinum model. We determined the course of infections in zebrafish is significantly more severe than in medaka. Together, these results indicate that the medaka-M. marinum model provides unique advantages for studying chronic mycobacteriosis.

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Section: Marinum Establishes An Acute or Chronic Infection In Medamentioning

confidence: 97%

Mycobacterium marinum produces long-term chronic infections in medaka: A new animal model for studying human tuberculosis

Broussard¹,

Ennis²

2007

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…In this report, we describe the results of a taxonomic study of these isolates and propose that they are representative of a novel species, Mycobacterium shottsii sp. nov. Isolate M175 T and 20 similar isolates were recovered from granulomatous lesions in striped bass (Rhodes et al, 2001). One isolate each (M23 and M216) was recovered from kidney and skin lesions and all others were from the spleen.…”

mentioning

confidence: 94%

“…An increased awareness of the diversity and complexity within the genus has resulted from the application of molecular techniques to the analysis of isolates from environmental sources and clinical specimens (Springer et al, 1993). During a recent epizootic of mycobacteriosis in striped bass, Morone saxatilis, from the Chesapeake Bay, various mycobacteria were isolated including a homogeneous group that, on the basis of traditional biochemical tests, mycolic acid analyses and a distinct 16S rRNA gene sequence, could not be assigned to any recognized species (Rhodes et al, 2001). More extensive characterization using additional biochemical tests, antimicrobial susceptibility and HPLC mycolic acid pattern analysis of additional isolates indicated that these isolates belong to a novel taxon.…”

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confidence: 99%

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Mycobacterium shottsii sp. nov., a slowly growing species isolated from Chesapeake Bay striped bass (Morone saxatilis)

Rhodes

Kator

Kotob

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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“…Aunque la sintomatología observada no permite asegurar que la infección es causada únicamente por Mycobacterium, las lesiones observadas son básicamente similares a las descritas en estudios realizados tanto en peces de agua dulce y marinos, ya sea por inducción experimental o por infecciones espontáneas de micobacteriosis (20)(21)(22)(23). Entre los signos descritos por la presencia de Mycobacterium se encuentran: pérdida de peso, necrosis y quistes en órganos internos, exoftalmia, inflamaciones c u t á n e a s , d e f o r m a c i o n e s e s q u e l é t i c a s especialmente en espina dorsal y mandíbula; y decoloración del cuerpo (6,20,24,25).…”

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Determinación y prevalencia de Mycobacterium spp., en tilapia nilótica (Oreochromis niloticus) cultivada en Campeche, México

et al. 2013

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RESUMENObjetivo. Determinar la presencia y prevalencia de Mycobacterium spp., en granjas de tilapia nilótica (Oreochromis niloticus) en el Municipio de Champotón, Campeche, México. Materiales y métodos. La colecta de organismos se realizó en tres granjas de cultivo de tilapia nilótica del municipio de Champotón, Campeche, México. Los organismos se examinaron externa e internamente y se tomó una muestra de riñón la cual fue sembrada en forma de estría en medios de cultivo: Löwesntein-Jensen, TCBS, KF y en TSA; las placas fueron incubadas a 35°C de 24 a 48 horas, los órganos fueron fijados en formalina tamponada al 10% para ser procesados para histología de rutina para análisis posteriores. Asimismo, muestras de cultivo bacteriológico y de tejido fueron teñidas con la técnica de Ziel-Neelsen con el fin de observar la presencia de bacilos ácido-alcohol resistentes. Resultados. Los resultados obtenidos sugieren que la presencia de Mycobacterium spp., es constante y en alta prevalencia y puede ser un factor que este mermando la rentabilidad del cultivo. Conclusiones. La presencia de Mycobacterium spp., representa un riesgo para el cultivo de tilapia en el municipio de Champotón, por ser una enfermedad muy persistente y difícil de erradicar una vez ocurrido e brote de infección, por lo cual es importante llevar estudios más detallados de la presencia de este género bacteriano, así como, medidas de prevención y dispersión de este patógeno en los cultivos adyacentes. ABSTRACTObjective. Determine the presence and prevalence of Mycobacterium spp., at tilapia (Oreochromis niloticus) farms in the municipality of Champotón, Campeche, Mexico. Materials and methods. The collection of organisms was conducted in three farms of Nile tilapia at Champotón township, Campeche, Mexico. The organisms were examined externally and internally and a kidney sample was inoculated in different growth media: Löwestein-Jensen, TCBS, KF and TSA. The plates were incubated at 35°C for 24 to 48 hours, the organs were fixed in 10% buffered formalin to be processed for routine histology for further analysis. Likewise, bacterial culture samples and tissue were stained through the Ziel-Neelsen technique to observe the presence of acid-fast bacilli. Results. The results suggest that the presence of Mycobacterium spp., is constant and its high prevalence and could be an undermining issue in the profitability of this culture. Conclusions. The presence of Mycobacterium spp., represents a risk for tilapia culture in the municipality of Champotón, it is a very persistent and difficult to eradicate disease once an outbreak has occurred, reason for which it is important to bring more detailed studies of the presence of this bacterial genus, as well as prevention and spread of this pathogen in adjacent cultures.

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A UniqueMycobacteriumSpecies Isolated from an Epizootic of Striped Bass (Morone saxatilis)

Cited by 51 publications

References 21 publications

Mycobacterium marinum produces long-term chronic infections in medaka: A new animal model for studying human tuberculosis

Mycobacterium marinum produces long-term chronic infections in medaka: A new animal model for studying human tuberculosis

Mycobacterium shottsii sp. nov., a slowly growing species isolated from Chesapeake Bay striped bass (Morone saxatilis)

Determinación y prevalencia de Mycobacterium spp., en tilapia nilótica (Oreochromis niloticus) cultivada en Campeche, México

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