A UCMPs@MIL-100 based thermo-sensitive molecularly imprinted fluorescence sensor for effective detection of β-lactoglobulin allergen in milk products

Abstract

In this study, a thermo-sensitive molecularly imprinted fluorescence sensor was developed for the specific detection of β-Lactoglobulin (β-LG) allergen in milk products. The metal–organic frameworks (MIL-100) with a high specific surface area was coated on the surface of upconversion micro-particles (UCMPs). As the core, an imprinted polymer layer allowing for swelling and shrinking with response to temperature was prepared, which exhibited high adsorption and mass transfer capabilities for β-LG allergen. The fluorescence intensity of UCMPs@MIL-100@MIP decreased linearly with the concentration of β-LG in the range of 0.1–0.8 mg mL−1, and the limit of detection was 0.043 mg mL−1. The imprinting factor reached 3.415, which indicated that excellent specificity of the UCMPs@MIL-100@MIP for β-LG allergen. In the analysis of β-LG allergen in actual milk samples, the proposed UCMPs@MIL-100@MIP fluorescence sensor produced reliable and accurate results (recovery: 86.0–98.4%, RSD: 2.8–6.8%), closely related to the results of standard HPLC method (correlation coefficient: 0.9949), indicating that its feasibility in the detection of β-LG allergen. Graphical Abstract

Ratiometric Fluorescence Capillary Sensor-Integrated Molecular Imprinting for Simultaneous Detection of Two Biological Indicators of Parkinson’s Disease

“…The limit of detection (LOD) was estimated to be 9.8 × 10 –10 M based on 3σ/ s . The above lower LOD of Si-CD@CdTe@CMIP toward HVA and Al 3+ illustrated that the Si-CD@CdTe@CMIP exhibited high sensitivity for trace detection of HVA and Al 3+ . , …”

“…The above lower LOD of Si-CD@CdTe@CMIP toward HVA and Al 3+ illustrated that the Si-CD@CdTe@CMIP exhibited high sensitivity for trace detection of HVA and Al 3+ . 27,28 In this work, the Si-CD@CdTe@CMIP for simultaneous detection of HVA and Al 3+ was further investigated, and the results are shown in Figure 2E and Figure S5E. Exposed to the mixture solution of HVA and Al 3+ , the fluorescence intensity of Si-CD@CdTe@CMIP was quenched with the addition of HVA and Al 3+ .…”

A fluorescence detection method for the determination of β-lactoglobulin in foods

“…The LOD of the developed assay was lower than for some of the others. [33][34][35]39,40 Its detection time was also shorter than for most of the assays. 32,[35][36][37][38][39][40] Fig.…”

“…[33][34][35]39,40 Its detection time was also shorter than for most of the assays. 32,[35][36][37][38][39][40] Fig. 3…”

Fluorescence detection of milk allergen β-lactoglobulin based on aptamers and WS2 nanosheets

“…Traditional chromatography is commonly used for β-Lg monitoring, including high performance liquid chromatography (HPLC), 10 ultra-high performance liquid chromatography (UHPLC), 11 and liquid chromatography tandem mass spectrometry (LC-MS/MS). 12 In addition, some novel detection methods, such as enzyme-linked immunosorbent assay, 13 photoelectrochemical immunoassay, 14 lateral flow immunoassay, 15 real-time quantitative polymerase chain reaction, 16 transient isotachophoresis, 17 and molecular imprinting, 18 have also been used for the detection of β-Lg. Although these reported methods have exhibited very good detection sensitivity and accuracy, there are still some disadvantages, such as the need for expensive analytical equipment and complex sample pre-treatment processes.…”