Ratiometric Fluorescence Capillary Sensor-Integrated Molecular Imprinting for Simultaneous Detection of Two Biological Indicators of Parkinson’s Disease

Abstract

This work proposed ratiometric fluorescence capillary sensing systemintegrated molecular imprinting with highly sensitive and selective detection for two biological indicators of Parkinson's disease (homovanillic acid (HVA) and Al 3+ ). In this research, the silicon carbon quantum dot and the near-infrared CdTe quantum dot as luminescence sources were doped to an imprinted layer, which was attached to the inner surface wall of an amino-functionalized capillary. The fluorescence emissions of the ratiometric fluorescence capillary-imprinted sensor at 434 and 707 nm were quenched by HVA, and only the fluorescence emission at 434 nm was quenched by Al 3+ . Ratiometric fluorescence capillary sensing system-integrated molecular imprinting was used to detect simultaneously HVA and Al 3+ with linearity over 1.0 × 10 −9 −2.5 × 10 −7 and 1.0 × 10 −9 −1.1 × 10 −7 M, respectively. The sensor showcased detection limitations of 8.7 × 10 −10 and 9.8 × 10 −10 M, indicating that the ratiometric fluorescence capillary sensing system-integrated molecular imprinting had great potential application for detecting HVA and Al 3+ in serum and urine samples. The ratiometric fluorescence capillary sensing system-integrated molecular imprinting achieved highly sensitive and selective detection of HVA and Al 3+ with a microvolume test dosage of 18 μL, which provided a new way for early diagnosis and disease monitoring of Parkinson's disease.

Sensitive and selective detection of p-nitroaniline with the assistance of a fluorescence capillary imprinted sensor

“…The fluorescence intensity of the CdTe@FMIP-CA sensor shows no change within 60 min, indicating that the CdTe@FMIP-CA sensor has good fluorescence stability. 26…”

“…The uorescence intensity of the CdTe@FMIP-CA sensor shows no change within 60 min, indicating that the CdTe@FMIP-CA sensor has good uorescence stability. 26 Fluorescence quenching mechanism. In this paper, to further explore the response mechanism between CdTe QDs toward p-NA, UV-vis spectra of CdTe QDs, p-NA, and CdTe@FMIP-CA sensor were investigated, as shown in Fig.…”

Facile synthesis of a fluorescent probe based on a terbium-based metal–organic framework for selective detection of Fe(iii) and Al(iii)

“…11,12 Most seriously, Al 3+ is extremely harmful to the human body, increasing the risk of Parkinson's disease, breast cancer and Alzheimer's disease. [13][14][15] Therefore, it is imperative to develop a sensitive and robust analytical method to detect the concentrations of Fe 3+ and Al 3+ .…”

Integrating Intelligent Logic Gate Dual-Nanozyme Cascade Fluorescence Capillary Imprinted Sensors for Ultrasensitive Simultaneous Detection of 2,4-Dichlorophenoxyacetic Acid and 2,4-Dichlorophenol

“…16 Especially, the fluorescence capillary analysis (FCA) method using capillary as an alternative fluorescence colorimetric cell can achieve the ultrasensitive determination of trace samples (18 μL/time) due to the waveguide effect of capillary tubes. 17,18 Nanozyme is a powerful nanomaterial with high catalytic activity. 19−21 Compared with traditional enzyme mimics and natural enzymes, nanozyme has the advantages of high stability, low production cost, and easy storage.…”

“…In addition, these methods suffer from the problems of complex instruments, high cost, time-consuming, and low selectivity. − Fluorescence sensor has been widely used in the field of analysis because of the high sensitivity toward targets . Especially, the fluorescence capillary analysis (FCA) method using capillary as an alternative fluorescence colorimetric cell can achieve the ultrasensitive determination of trace samples (18 μL/time) due to the waveguide effect of capillary tubes. , …”