Fluorescence detection of milk allergen β-lactoglobulin based on aptamers and WS₂ nanosheets

Abstract

β-lactoglobulin (β-Lg), a food allergen, can easily cause allergic reactions in infants and young children. Therefore, it is necessary to develop a rapid, sensitive, and selective detection method to protect...

Imprinting of nanoparticles in thin films: Quo Vadis?

“…The motivation to imprint the above mentioned biological entities or NPs has been primarily for the development of a lowcost and fast platform for different applications such as separation, sensing, etc [64][65][66][67][68] . However, the urge for imprinting with higher dimensions poses difficulties in the extraction and rebinding steps, as a result of the relatively lower diffusion of the large object as compared with molecules.…”

Highly Sensitive β-Lactoglobulin Fluorescent Aptamer Biosensors Based on Tungsten Disulfide Nanosheets and DNase I-Assisted Signal Amplification

“…The actual samples included milk and infant formula samples. These samples were pretreated according to previous reports [44]. For milk samples, the following steps were followed: The samples were heated at 40 • C for 30 min and then centrifuged at 8000 rpm for 20 min.…”

A rapid and sensitive aptamer-based biosensor for beta-lactoglobulin in milk

“…14 In addition, various techniques have been utilized to develop aptasensors for β-Lg detection, including fluorescence detection, 15,16 surface-enhanced Raman spectroscopy, 17 electrochemistry, 18 and transient synchronous electrophoresis. 19 Hong et al 20 employed a WS 2 nanosheet-based aptasensor for the fluorescence detection of β-Lg with an LOD of 20.4 ng mL −1 . Xu's group 21 established an electrochemical platform based on a highly selective DNA aptamer to detect β-Lg with an LOD of 0.007 ng mL −1 .…”