A two-photon fluorescence self-reporting black phosphorus nanoprobe for the <i>in situ</i> monitoring of therapy response

Guan, Kesong; Wang, Peng; Wang, Youjuan; Liu, Hong-Wen; Xie, Qingji; Song, Guohe; Yin, Xiang; Huan, Shuangyan; Zhang, Xiaobing

doi:10.1039/d0cc05335j

Cited by 10 publications

(7 citation statements)

References 29 publications

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Section: Cancer Stratificationmentioning

confidence: 99%

“…Guan et al reported a black phosphorus-based two-photon nanoprobe (TPBP), the 1 O 2 generated by TPBP activated the two-photon molecule to emit a fluorescence signal under laser irradiation, and the tumor growth inhibition rate was positively correlated with the fluorescence signal (R 2 = 0.989). 188 Wang et al reported a photosensitizer called NG-cRGD, which consisted of a cyclic pentapeptide (cRGD) that specifically targeted integrin avb3 in tumors, and a 2-cyano-4nitrobenzene-1-sulfonyl group that was responsive to glutathione. 189 NG-cRGD incorporated a strong electronabsorbing group that effectively inhibited the breaking of CQC bonds and the associated phototoxicity.…”

Section: Pdtmentioning

confidence: 99%

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Nanoprobe-based molecular imaging for tumor stratification

Ma,

Mao,

et al. 2023

Chem. Soc. Rev.

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Section: Cancer Stratificationmentioning

confidence: 99%

Section: Pdtmentioning

confidence: 99%

Nanoprobe-based molecular imaging for tumor stratification

Ma,

Mao,

et al. 2023

Chem. Soc. Rev.

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“…The 1 O 2 generation was measured using DPBF as a capture agent after treatment with light irradiation of PLU@PTc hydrogels (PLU@PTc/light) and PTc NPs (PTc/light) using PLU@PTc and PTc treatments as controls. 26 Briefly, hydrogel disks (ϕ 8 mm × 2 mm) were first incubated in pH 5.5 buffers for 4 h, and then 10 mL of DPBF solution in dimethyl sulfoxide (20 μg/mL) was added in the dark for 1 h. The mixture was exposed to a 660 nm laser for different time periods, and the UV−Vis absorption spectra were recorded.…”

Section: 7mentioning

confidence: 99%

On-Demand Changeable Theranostic Hydrogels and Visual Imaging-Guided Antibacterial Photodynamic Therapy to Promote Wound Healing

Ran

Zheng

Cao

et al. 2022

ACS Appl. Mater. Interfaces

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Antibacterial wound dressings are confronted with the challenges in real-time imaging of infected wounds and effective removal of bacterial debris after sterilization to promote the healing process. Herein, injectable theranostic hydrogels were constructed from antimicrobial peptide ε-polylysine (ePL) and polydopamine (PDA) nanoparticles for real-time diagnosis of infected wounds, imaging-guided antibacterial photodynamic therapy (PDT), and on-demand removal of bacterial debris. Ureido-pyrimidinone was conjugated on ePL to produce PLU hydrogels through quadruple hydrogen bonding, and the inoculation of tetrakis(4-carboxyphenyl)porphyrin (TCPP)-loaded PDA (PTc) nanoparticles introduced Schiff base linkages in PLU@PTc hydrogels. The double-cross-linked networks enhance mechanical performance, adhesion strength, and self-healing properties of hydrogels, and the dynamic cross-linking enables their photothermal removal. The injection of PLU precursors and PTc NPs generates in situ sol−gel transformation, and the acid-triggered release of TCPP restores fluorescence emissions for real-time imaging of infected wounds under 410 nm illumination. Then, the released TCPP in the infected wounds is illuminated at 660 nm to launch a precise antibacterial PDT, which is strengthened by the bacterial capture on hydrogels. Hydrogels with wrapped bacterial debris are removed under illumination at 808 nm, and the hydrogel dressing change accelerates healing of infected wounds through simultaneous relief of oxidative stress, regulation of inflammatory factors, acceleration of collagen deposition, and promotion of angiogenesis. Thus, this study demonstrates a feasible strategy for wound infection theranostics through bacterial infection-triggered visual imaging, efficient nonantibiotic sterilization, and on-demand dressing change and bacterial debris removal.

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“…A growing number of studies have demonstrated that highly activated photosensitizers can destroy normal cells, compromising therapeutic efficacy . Moreover, the post-PDT safety problem means the disposition of the photosensitizers by body after therapy, which has attracted increasing attention in recent years. − Obviously, real-time monitoring of photosensitizers in the body and evaluating the exact treatment end point are especially important for PDT under dosimetric guidance. − Unfortunately, additional fluorescent probes need to be introduced into most current PDTs, which makes the monitoring process complicated and delayed.…”

Section: Introductionmentioning

confidence: 99%

Self-Immolative Photosensitizers for Self-Reported Cancer Phototheranostics

Wang¹,

Sun²,

Huang³

et al. 2023

J. Am. Chem. Soc.

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Photosensitizers to precise target and change fluorescence upon light illumination could accurately self-report where and when the photosensitizers work, enabling us to visualize the therapeutic process and precisely regulate treatment outcomes, which is the unremitting pursuit of precision and personalized medicine. Here, we report self-immolative photosensitizers by adopting a strategy of light-manipulated oxidative cleavage of CC bonds that can generate a burst of reactive oxygen species, to cleave to release self-reported red-emitting products and trigger nonapoptotic cell oncosis. Strong electron-withdrawing groups are found to effectively suppress the CC bond cleavage and phototoxicity via studying the structure–activity relationship, allowing us to elaborate NG1–NG5 that could temporarily inactivate the photosensitizer and quench the fluorescence by different glutathione (GSH)-responsive groups. Thereinto, NG2 with 2-cyano-4-nitrobenzene-1-sulfonyl group displays excellent GSH responsiveness than the other four. Surprisingly, NG2 shows better reactivity with GSH in weakly acidic condition, which inspires the application in weakly acidic tumor microenvironment where GSH elevates. To this end, we further synthesize NG-cRGD by anchoring integrin αvβ3 binding cyclic pentapeptide (cRGD) for tumor targeting. In A549 xenografted tumor mice, NG-cRGD successfully deprotects to restore near-infrared fluorescence because of elevated GSH in tumor site, which is subsequently cleaved upon light irradiation releasing red-emitting products to report photosensitizer working, while effectively ablating tumors via triggered oncosis. The advanced self-immolative organic photosensitizer may accelerate the development of self-reported phototheranostics in future precision oncology.

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A two-photon fluorescence self-reporting black phosphorus nanoprobe for the in situ monitoring of therapy response

Abstract: The in situ and real-time supervising of reactive oxygen species (ROS) generated during the photodynamic therapy (PDT) is of great significance for lessening the nonspeciﬁc damage and guiding personalized therapy....

Cited by 10 publications

References 29 publications

Nanoprobe-based molecular imaging for tumor stratification

Nanoprobe-based molecular imaging for tumor stratification

On-Demand Changeable Theranostic Hydrogels and Visual Imaging-Guided Antibacterial Photodynamic Therapy to Promote Wound Healing

Self-Immolative Photosensitizers for Self-Reported Cancer Phototheranostics

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