A Turn-ON fluorometric biosensor based on ssDNA immobilized with a metal phenolic nanomaterial for the sequential detection of Pb(ii) and epirubicin cancer drug

Abstract

In this paper, we propose a fluorescent biosensor for the sequential detection of Pb2+ ions and the cancer drug epirubicin (Epn) using the interactions between label-free guanine-rich ssDNA (LFGr-ssDNA), acridine orange (AO), and a metal–phenolic nanomaterial.

Recyclable Target Metal-Enhanced Fluorometric Naked Eye Aptasensor for the Detection of Pb2+ and Ag+ Ions Based on the Structural Change of CaSnO3@PDANS-Constrained GC-Rich ssDNA

“…Moreover, currently, fluorescence, electrochemiluminescence, photoluminescence, colorimetric, and SPR methods were used, but these common optical methods also have some limitations, including time consumption, low compatibility, frequent electrode fouling, low reproducibility, selectivity, and stability. In response to these limitations, the past decades have seen great progress in the development of heavy-metal sensors based on guanine–lead [G4–Pb 2+ ] and cytosine–silver [C–Ag + –C] coordination chemistry using colorimetric, fluorescence, and electrochemical detection techniques, ,,− among which the fluorescence technique is the most convenient, economically feasible, and companionable for the sensitive and specific detection of Pb 2+ and Ag + ions.…”

“…Recently, increased attention is focused on the label or label-free DNA-based fluorescence biosensor using various nanomaterials , for the detection of targets because of their unique chemical or physical properties. ,, Currently, three kinds of approaches involving the nanomaterial-based DNA fluorescence method are established for the detection of toxic metal exposure: The first approach involves the usage of DNA as a specific binding ligand for metal sensing due to extreme specific coordination interaction between the metal (Pb 2+ and Ag + ) and DNA base pairs. Guschlbauer et al reported that Pb 2+ could interact with the guanine (G) base to form a stable G-quadruplex structure via G4–Pb 2+ from the random coil DNA structure.…”

Toward a Selective Analysis of Heavy Metal Salts in Aqueous Media with a Fluorescent Probe Array

“…Upon excitation at wavelengths of 450 nm, corresponding to its absorption maximum at around 260 nm, we observed an increase in its fluorescence upon addition of high lead concentrations from 3.26 × 10 −4 M. This observed increase in fluorescence under high [Pb 2+ ] concentrations cannot be explained by the effect of a heavy atom. However, there are some literature data showing that Pb can enhance fluorescence of probes under certain conditions [68][69][70].…”

High Catalytic Activity of Fluorophore-Labeled Y-Shaped DNAzyme/3D MOF-MoS2NBs as a Versatile Biosensing Platform for the Simultaneous Detection of Hg2+, Ni2+, and Ag+ Ions

“…In particular, the fluorescent sensor exhibits convenient advantages such as metal ion detection with remarkable superiority, sensitivity, low cost, easy operation, and efficiency. The fluorescent sensors have attracted extensive attention. , A number of fluorescent biosensors for heavy metal detection are reported over the past several years. , However, sensitive and selective fluorescent sensors for multiple detections of Hg 2+ , Ni 2+ , and Ag + ions are scarce. Frequently, significant practical limitations of Hg 2+ , Ni 2+ , and Ag + ions are seen, which include detailed synthesis schemes, high-cost methods, and insufficient selectivity.…”

“…12,13 A number of fluorescent biosensors for heavy metal detection are reported over the past several years. 14,15 However, sensitive and selective fluorescent sensors for multiple detections of Hg 2+ , Ni 2+ , and Ag + ions are scarce. Frequently, significant practical limitations of Hg 2+ , Ni 2+ , and Ag + ions are seen, which include detailed synthesis schemes, high-cost methods, and insufficient selectivity.…”