A Transcriptional Activator, AoXlnR, Controls the Expression of Genes Encoding Xylanolytic Enzymes in Aspergillus oryzae

Marui, Junichiro; Tanaka, Akimitsu; Mimura, Satoshi; Graaff, L.H. de; Visser, Jaap; Kitamoto, Noriyuki; Kato, Masashi; Kobayashi, Tetsuo; Tsukagoshi, Norihiro

doi:10.1006/fgbi.2001.1321

Cited by 85 publications

(62 citation statements)

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“…We further assessed the phenotype of 34 strains containing deletions of predicted transcription factor (TF) genes that showed induction when N. crassa is exposed to xylan and identified a predicted ortholog of xlnR/xyr1 (NCU06971; xlr-1). In Hypocrea jecorina (Trichoderma reesei) and Aspergillus spp., the transcriptional regulator XYR1/XlnR, respectively, regulates expression of both hemicellulase and cellulase genes (12,31,34,38,51,52,61). Lossof-function mutants in xlnR in Aspergillus niger exhibit strongly reduced xylanolytic activities (64).…”

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Deciphering Transcriptional Regulatory Mechanisms Associated with Hemicellulose Degradation in Neurospora crassa

et al. 2012

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ABSTRACTHemicellulose, the second most abundant plant biomass fraction after cellulose, is widely viewed as a potential substrate for the production of liquid fuels and other value-added materials. Degradation of hemicellulose by filamentous fungi requires production of many different enzymes, which are induced by biopolymers or its derivatives and regulated mainly at the transcriptional level through transcription factors (TFs).Neurospora crassa, a model filamentous fungus, expresses and secretes enzymes required for plant cell wall deconstruction. To better understand genes specifically associated with degradation of hemicellulose, we applied secretome and transcriptome analysis toN. crassagrown on beechwood xylan. We identified 34 secreted proteins and 353 genes with elevated transcription on xylan. The xylanolytic phenotype of strains with deletions in genes identified from the secretome and transcriptome analysis of the wild type was assessed, revealing functions for known and unknown proteins associated with hemicellulose degradation. By evaluating phenotypes of strains containing deletions of predicted TF genes inN. crassa, we identified a TF (XLR-1;xylan degradationregulator1) essential for hemicellulose degradation that is an ortholog to XlnR/XYR1 inAspergillusandTrichodermaspecies, respectively, a major transcriptional regulator of genes encoding both cellulases and hemicellulases. Deletion ofxlr-1inN. crassaabolished growth on xylan and xylose, but growth on cellulose and cellulolytic activity were only slightly affected. To determine the regulatory mechanisms for hemicellulose degradation, we explored the transcriptional regulon of XLR-1 under xylose, xylanolytic, and cellulolytic conditions. XLR-1 regulated only some predicted hemicellulase genes inN. crassaand was required for a full induction of several cellulase genes. Hemicellulase gene expression was induced by a combination of release from carbon catabolite repression (CCR) and induction. This systematic analysis illustrates the similarities and differences in regulation of hemicellulose degradation among filamentous fungi.

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Deciphering Transcriptional Regulatory Mechanisms Associated with Hemicellulose Degradation in Neurospora crassa

et al. 2012

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“…No data on the expression of xyn4 are available. Xylanase expression by D-xylose is regulated via the transcriptional activator XYR1 or its orthologue XlnR and by general carbon catabolite (de)repression in T. reesei and other fungi, including, e.g., Aspergillus spp., Neurospora crassa, and Fusarium spp., although species-specific adaptations are found (12)(13)(14)(15)(16)(17)(18). In T. reesei, xyn1 and xyn2 respond to carbon catabolite repression in different ways (19).…”

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Xylanase Gene Transcription in Trichoderma reesei Is Triggered by Different Inducers Representing Different Hemicellulosic Pentose Polymers

et al. 2013

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“…CreA is a well-known transcriptional repressor involved in carbon catabolite repression, that strongly suppresses cellulolytic enzyme production in Aspergillus species in the presence of glucose. [5][6][7][8] XlnR, a transcriptional regulator of xylanolytic enzyme genes, 9,10) positively regulates the expression of genes for cellulolytic enzymes in A. niger and A. oryzae, 11,12) but the regulatory mechanisms of cellulolytic enzyme genes in A. oryzae are very complex and not fully understood.…”

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ManR, a Transcriptional Regulator of the β-Mannan Utilization System, Controls the Cellulose Utilization System in <i>Aspergillus oryzae</i>

Ogawa

Kobayashi

Koyama

2013

Bioscience, Biotechnology, and Biochemistry

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ManR, a DNA-binding protein possessing the Zn(II) 2 Cys 6 binuclear cluster domain, acts as a positive regulator of mannanolytic enzyme genes in Aspergillus oryzae. In this study, we found that ManR controlled the expression not only of mannanolytic enzyme genes but also of cellulolytic enzyme genes in A. oryzae, based on DNA microarray analysis of a manR-disruptant and a manR-overexpressing strain. A new model for the regulation of cellulase and hemicellulase genes mediated by ManR and XlnR in A. oryzae is proposed.

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A Transcriptional Activator, AoXlnR, Controls the Expression of Genes Encoding Xylanolytic Enzymes in Aspergillus oryzae

Cited by 85 publications

References 47 publications

Deciphering Transcriptional Regulatory Mechanisms Associated with Hemicellulose Degradation in Neurospora crassa

Deciphering Transcriptional Regulatory Mechanisms Associated with Hemicellulose Degradation in Neurospora crassa

Xylanase Gene Transcription in Trichoderma reesei Is Triggered by Different Inducers Representing Different Hemicellulosic Pentose Polymers

ManR, a Transcriptional Regulator of the β-Mannan Utilization System, Controls the Cellulose Utilization System in <i>Aspergillus oryzae</i>

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