ManR, a Transcriptional Regulator of the β-Mannan Utilization System, Controls the Cellulose Utilization System in &lt;i&gt;Aspergillus oryzae&lt;/i&gt;

Ogawa, Mitsuhiro; Kobayashi, Tetsuo; Koyama, Yasuji

doi:10.1271/bbb.120795

Cited by 62 publications

(42 citation statements)

References 18 publications

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“…Despite differences in function have been reported, this prevalence suggests partially conserved regulatory mechanisms involved in cellulose utilization [98, 114]. These regulators have been fully characterized in N. crassa (CLR-1/2) and partially in A. nidulans (ClrA/B) [98, 115], A. niger [114], and A. oryzae (ManR) [116, 117], but only poorly in Talaromyces cellulolyticus (tCLB2) [118] and Penicillium oxalicum (CLR2) [25], showing both similarities and differences.…”

Section: Transcription Factors Directly Involved In Plant Biomass Degmentioning

confidence: 99%

Regulators of plant biomass degradation in ascomycetous fungi

Benocci

Pontes

Zhou

et al. 2017

Biotechnol Biofuels

179

158

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Fungi play a major role in the global carbon cycle because of their ability to utilize plant biomass (polysaccharides, proteins, and lignin) as carbon source. Due to the complexity and heterogenic composition of plant biomass, fungi need to produce a broad range of degrading enzymes, matching the composition of (part of) the prevalent substrate. This process is dependent on a network of regulators that not only control the extracellular enzymes that degrade the biomass, but also the metabolic pathways needed to metabolize the resulting monomers. This review will summarize the current knowledge on regulation of plant biomass utilization in fungi and compare the differences between fungal species, focusing in particular on the presence or absence of the regulators involved in this process.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0841-x) contains supplementary material, which is available to authorized users.

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Section: Transcription Factors Directly Involved In Plant Biomass Degmentioning

confidence: 99%

Regulators of plant biomass degradation in ascomycetous fungi

Benocci

Pontes

Zhou

et al. 2017

Biotechnol Biofuels

179

158

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“…In Neurospora crassa , the transcription factors CLR1 and CLR2 are essential for growth on cellulose and are required for expression of a ∼212 gene regulon that is induced in response to cellodextrins, such as cellobiose [8], [9] (Figure 1). In Aspergillus nidulans and A. oryzae , a clr-2 homolog, called clrB/manR , respectively, is also essential for cellulase gene expression and activity [8], [10], [11]. Additional transcriptional regulators that promote expression of some genes encoding hydrolytic enzymes have also been identified, including mcmA in A. nidulans [12], clbR in A. aculeatus [13], and aceII and bglR in T. reesei [14], [15].…”

Section: Introductionmentioning

confidence: 99%

VIB1, a Link between Glucose Signaling and Carbon Catabolite Repression, Is Essential for Plant Cell Wall Degradation by Neurospora crassa

2014

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Filamentous fungi that thrive on plant biomass are the major producers of hydrolytic enzymes used to decompose lignocellulose for biofuel production. Although induction of cellulases is regulated at the transcriptional level, how filamentous fungi sense and signal carbon-limited conditions to coordinate cell metabolism and regulate cellulolytic enzyme production is not well characterized. By screening a transcription factor deletion set in the filamentous fungus Neurospora crassa for mutants unable to grow on cellulosic materials, we identified a role for the transcription factor, VIB1, as essential for cellulose utilization. VIB1 does not directly regulate hydrolytic enzyme gene expression or function in cellulosic inducer signaling/processing, but affects the expression level of an essential regulator of hydrolytic enzyme genes, CLR2. Transcriptional profiling of a Δvib-1 mutant suggests that it has an improper expression of genes functioning in metabolism and energy and a deregulation of carbon catabolite repression (CCR). By characterizing new genes, we demonstrate that the transcription factor, COL26, is critical for intracellular glucose sensing/metabolism and plays a role in CCR by negatively regulating cre-1 expression. Deletion of the major player in CCR, cre-1, or a deletion of col-26, did not rescue the growth of Δvib-1 on cellulose. However, the synergistic effect of the Δcre-1; Δcol-26 mutations circumvented the requirement of VIB1 for cellulase gene expression, enzyme secretion and cellulose deconstruction. Our findings support a function of VIB1 in repressing both glucose signaling and CCR under carbon-limited conditions, thus enabling a proper cellular response for plant biomass deconstruction and utilization.

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“…For example, XLR1/XlnR is critical for xylanase production in N. crassa and Fusarium graminearum (Brunner et al, 2007; Sun et al, 2012a) and for both hemicellulase and cellulase production in T. reesei and Aspergillus niger (Mach-Aigner et al, 2008; Stricker et al, 2008; van Peij et al, 1998). In N. crassa , CLR1 and CLR2 are essential for cellulase production, as is a clr-2 ortholog in Aspergillus nidulans (ClrB) and Aspergillus oryzae (ManR) (Coradetti et al, 2012; Ogawa et al, 2013). However, simple manipulation of the transcript level of an individual transcriptional activator to achieve high cellulolytic enzyme production in the absence of inducers derived from plant biomass has only been successful with a single-point-mutation in xyr-1 in T. reesei and via mis-expression of clr-2 in N. crassa (Coradetti et al, 2013; Derntl et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation

Xiong¹,

Coradetti²,

Li³

et al. 2014

Fungal Genetics and Biology

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Improving cellulolytic enzyme production by plant biomass degrading fungi holds great potential in reducing costs associated with production of next-generation biofuels generated from lignocellulose. How fungi sense cellulosic materials and respond by secreting enzymes has mainly been examined by assessing function of transcriptional regulators and via transcriptional profiling. Here, we obtained global proteomic and phosphoproteomic profiles of the plant biomass degrading filamentous fungus Neurospora crassa grown on different carbon sources, i.e. sucrose, no carbon, and cellulose, by performing isobaric tags for relative and absolute quantification (iTRAQ)-based LC–MS/MS analyses. A comparison between proteomes and transcriptomes under identical carbon conditions suggests that extensive post-transcriptional regulation occurs in N. crassa in response to exposure to cellulosic material. Several hundred amino acid residues with differential phosphorylation levels on crystalline cellulose (Avicel) or carbon-free medium vs sucrose medium were identified, including phosphorylation sites in a major transcriptional activator for cellulase genes, CLR1, as well as a cellobionic acid transporter, CBT1. Mutation of phosphorylation sites on CLR1 did not have a major effect on transactivation of cellulase production, while mutation of phosphorylation sites in CBT1 increased its transporting capacity. Our data provides rich information at both the protein and phosphorylation levels of the early cellular responses to carbon starvation and cellulosic induction and aids in a greater understanding of the underlying post-transcriptional regulatory mechanisms in filamentous fungi.

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ManR, a Transcriptional Regulator of the β-Mannan Utilization System, Controls the Cellulose Utilization System in <i>Aspergillus oryzae</i>

Cited by 62 publications

References 18 publications

Regulators of plant biomass degradation in ascomycetous fungi

Regulators of plant biomass degradation in ascomycetous fungi

VIB1, a Link between Glucose Signaling and Carbon Catabolite Repression, Is Essential for Plant Cell Wall Degradation by Neurospora crassa

The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation

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