A tracer-type fluorescent probe for imaging adenosine triphosphate under the stresses of hydrogen sulfide and hydrogen peroxide in living cells

Abstract

Adenosine triphosphate (ATP) is a direct energy source in cells and the core of the biochemical system, which is closely related to various metabolisms in the organism. Therefore, designing a...

A COX2-targeting cancer-specific fluorescent probe for hydrogen sulfide detection in living cells, Caenorhabditis elegans, and zebrafish

“…11 In this regard, many fluorescent probes specific for H 2 S detection have been developed. [12][13][14][15][16][17][18][19][20][21] In our previous work, a H 2 S triggered twophoton ratiometric fluorescent theranostic probe was also designed and studied in vitro and in vivo. 22 Unfortunately, these probes failed to differentiate tumor tissues from normal tissues, which is a considerable limitation for clinical application.…”

Review of Chemical Sensors for Hydrogen Sulfide Detection in Organisms and Living Cells

“…[ 13 ]. At the same time, fluorescence and colorimetric chemical sensors exhibit the advantages of low cost, visualization, and being non-invasive, which bestow them with an irreplaceable role in the real-time detection of H 2 S in vivo [ 14 , 15 , 16 ]. These chemical sensors usually rely on three main properties, including metal affinity, reducibility, and nucleophilicity [ 17 ].…”

An ICT-switched fluorescent probe for visualizing lipid and HClO in lipid droplets during ferroptosis

“…In recent years, fluorescence imaging technology has emerged to become a promising tool for tracking and detecting LDs and HClO in live cells due to its advantages of non-invasiveness, high sensitivity, and its ability to achieve in situ real-time detection at the cellular level in vivo. [14][15][16][17] There are currently a few commercial dyes used to target LDs, the most common of which are Nile red and BODIPY 493/503, 18,19 but there are some shortcomings in their practical biological applications; similarly, many fluorescent probes for detecting HClO have been reported, but improvements are still needed in their sensitivity, response time, and so on. 20,21 Thus, developing a method to monitor lipid accumulation and HClO in LDs would have important value in biomedical research.…”