A Theory of Energy Transfer in the Photosynthetic Unit

Bay, Z.; Pearlstein, Robert M.

doi:10.1073/pnas.50.6.1071

Cited by 68 publications

(13 citation statements)

References 14 publications

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“…The modern quantitative picture of the PSU began to take shape with the careful application of the R -6 theory to extensive in vivo experiments (Duysens 1952). It began to mature with the help of a more complete description of diffusive exciton flow (Bay and Pearlstein 1963;Duysens 1964;Pearlstein 1966), a description that recognized the stochastic nature of the transport process, as in the dynamics of Figure 2a. It has been the basis of most modeling in the field for thirty years, and is grounded firmly on Arnold and Oppenheimer's original physics.…”

Section: The Exciton Modelmentioning

confidence: 99%

Electronic excitation transfer in the photosynthetic unit: Reflections on work of William Arnold

Knox

1996

Photosynth Res

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Among his many contributions to photosynthesis, William Arnold made critical suggestions about the mechanism of the initial stages of excitation energy transfer and its measurement. Thus he helped found not only the general concept of the photosynthetic unit but also the key idea behind the detailed functional aspects of its 'chlorophyll antenna'. We review the development of these ideas and the modern form in which they have emerged.

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Section: The Exciton Modelmentioning

confidence: 99%

Electronic excitation transfer in the photosynthetic unit: Reflections on work of William Arnold

Knox

1996

Photosynth Res

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“…During the calculation of this parameter it should be taken into account that a single donor could potentially transfer energy to more than one acceptors. In this case Equation 6 transforms to (37,38),…”

Section: Methodsmentioning

confidence: 99%

Conformational and Dynamic Differences between Actin Filaments Polymerized from ATP- or ADP-Actin Monomers

Nyitrai

Hild

Hartvig

et al. 2000

Journal of Biological Chemistry

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Conformational and dynamic properties of actin filaments polymerized from ATP-or ADP-actin monomers were compared by using fluorescence spectroscopic methods. The fluorescence intensity of IAEDANS attached to the Cys 374 residue of actin was smaller in filaments from ADP-actin than in filaments from ATPactin monomers, which reflected a nucleotide-induced conformational difference in subdomain 1 of the monomer. Radial coordinate calculations revealed that this conformational difference did not modify the distance of Cys 374 from the longitudinal filament axis. Temperaturedependent fluorescence resonance energy transfer measurements between donor and acceptor molecules on Cys 374 of neighboring actin protomers revealed that the inter-monomer flexibility of filaments assembled from ADP-actin monomers were substantially greater than the one of filaments from ATP-actin monomers. Flexibility was reduced by phalloidin in both types of filaments.Actin is one of the most abundant proteins in biological systems and responsible for a number of cell functions in vivo (1, 2). Two principal forms of actin exist in living cells, the monomeric and the filamentous. Actins biological function depends on the actual dynamic and conformational properties of the protein and on the dynamic equilibrium between the two principal forms (1, 2).The polymerization of actin monomers with bound ATP is accompanied by the parallel hydrolysis of the nucleotide. However, the biological relevance of ATP hydrolysis by actin is still not well understood. Interestingly, ADP-monomeric actin also forms filaments (3), therefore, the presence of ATP and its hydrolysis are not essential for filament assembly. Previously, the hydrolysis of actin-bound ATP was assumed to play a key role in the steady-state treadmilling of actin filaments (4). Alternatively, Carlier (5, 6) suggested that ATP hydrolysis facilitated the rapid de-polymerization of actin.Recently, Janmey and colleagues (7) provided evidence that actin filaments polymerized from ATP-actin monomers were significantly stiffer than the ones obtained from ADP-monomers. Considering that both types of filaments consist mainly of ADP-actin protomers, this observation was explained by the assumption that the ATP-actin monomers were conformationally trapped following the hydrolysis of ATP, and the energy released during the hydrolysis was stored as elastic energy (7). The authors proposed that this elastic energy could play an important role when the actin filament interacted with actinbinding proteins. Although this exciting observation was later supported by other laboratories, a number of experimental results were contradictory.The direct effect of ATP on actin filaments was confirmed and extended to phalloidin-labeled actin by fluorescence microscopy experiments (8). Three-dimensional reconstructions from electron micrographs revealed similar effects of the nucleotides on the flexibility of actin filament (9, 10). The results of phosphorescence spectroscopic experiments apparently also supported the c...

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“…The excitons would decay before reaching the center, so Franck and Teller concluded that the unit and the participation of excitons could not exist. Work by Bay and Pearlstein 9,10 and by Duysens,6 relying on Förster's theory that we shall discuss below, reversed this conclusion. Nowadays, "excitons in photosynthesis" is virtually an industry.…”

Section: Excitation Transfer 1930 To 1945mentioning

confidence: 84%

Förster’s resonance excitation transfer theory: not just a formula

Knox

2012

J. Biomed. Opt.

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Abstract. After 65 years of increasing scrutiny and application, Theodor Förster's treatment of resonance excitation transfer is widely quoted and has acquired the acronym FRET, in which "F" originally and rather curiously stood for "fluorescence." In this brief and mostly qualitative survey, we review some of its history, mention its important limitations, and relate some personal encounters with Förster.

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A Theory of Energy Transfer in the Photosynthetic Unit

Cited by 68 publications

References 14 publications

Electronic excitation transfer in the photosynthetic unit: Reflections on work of William Arnold

Electronic excitation transfer in the photosynthetic unit: Reflections on work of William Arnold

Conformational and Dynamic Differences between Actin Filaments Polymerized from ATP- or ADP-Actin Monomers

Förster’s resonance excitation transfer theory: not just a formula

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