A Synthetic Oligopeptide Derived From Enamel Matrix Derivative Promotes the Differentiation of Human Periodontal Ligament Stem Cells Into Osteoblast‐Like Cells With Increased Mineralization

Kato, Hirohito; Katayama, Nobuhito; Taguchi, Yoichiro; Tominaga, Kazuhiro; Umeda, Makoto; Tanaka, Akio

doi:10.1902/jop.2012.120469

Cited by 35 publications

(38 citation statements)

References 40 publications

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“…In our previous studies, SP promoted cell proliferation of rat bone marrow stromal cells [18] and PDL fibroblasts [17,19]. SP also promoted cell proliferation of PDL stem cells [20]. In the current study, SP promoted cell proliferation of MSCs.…”

Section: Resultssupporting

confidence: 56%

“…EMD promotes OCN mRNA expression in C2C12 cells [29], and enamel matrix proteins (EMPs) extracted from swine promote OCN mRNA expression in mouse bone marrow stromal cells [38]. SP also promotes the production of OCN in PDL fibroblasts [19] and PDL stem cells [20]. In our study, SP promoted OCN production in MSCs.…”

Section: Resultsmentioning

confidence: 62%

“…Moreover, we have reported that SP promotes cell proliferation of human PDL fibroblasts [17] and rat bone marrow stromal cells [18]. SP also induced osteoblastic differentiation and hard tissue formation of PDL fibroblasts [19] and PDL stem cells [20]. These findings may contribute to the formation of new hard tissue and connective tissue, which is essential for periodontal tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

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The Effects of Synthetic Oligopeptide Derived from Enamel Matrix Derivative on Cell Proliferation and Osteoblastic Differentiation of Human Mesenchymal Stem Cells

Katayama

Kato

Taguchi

et al. 2014

IJMS

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Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to batch, and the use of a synthetic peptide could avoid use from an animal source, a completely synthetic peptide (SP) containing the active component of EMD would be useful. In this study an oligopeptide synthesized derived from EMD was evaluated for whether it contributes to periodontal tissue regeneration. We investigated the effects of the SP on cell proliferation and osteoblast differentiation of human mesenchymal stem cells (MSCs), which are involved in tissue regeneration. MSCs were treated with SP (0 to 1000 ng/mL), to determine the optimal concentration. We examined the effects of SP on cell proliferation and osteoblastic differentiation indicators such as alkaline phosphatase activity, the production of procollagen type 1 C-peptide and osteocalcin, and on mineralization. Additionally, we investigated the role of extracellular signal-related kinases (ERK) in cell proliferation and osteoblastic differentiation induced by SP. Our results suggest that SP promotes these processes in human MSCs, and that ERK inhibitors suppress these effects. In conclusion, SP promotes cell proliferation and osteoblastic differentiation of human MSCs, probably through the ERK pathway.

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Section: Resultssupporting

confidence: 56%

Section: Resultsmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

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The Effects of Synthetic Oligopeptide Derived from Enamel Matrix Derivative on Cell Proliferation and Osteoblastic Differentiation of Human Mesenchymal Stem Cells

Katayama

Kato

Taguchi

et al. 2014

IJMS

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“…Considering the source of the tissue, the distribution of studies was as follows: four from the periodontal ligament (8–11), four from the dental pulp (12–15), two from apical papilla (15, 16), two from alveolar bone (17, 18), one from maxillary sinus (19), periodontal ligament granulation tissue (20) and gingiva (18) each. Considering the most common and feasible source of stem cells, from the articles obtained, it can be said that most commonly, sources like periodontal ligament and dental pulp tissue was used for isolation of stem cells and was depicted in Fig.…”

Section: Resultsmentioning

confidence: 99%

Methods of Isolation and Characterization of Stem Cells from Different Regions of Oral Cavity Using Markers: A Systematic Review

Kavarthapu

Malaiappan

Dooraiswamy

2017

IJSC

111

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BackgroundPeriodontitis is a destructive inflammatory disorder of the periodontium caused by the destruction of periodontal tissues namely the PDL, cementum, alveolar bone, and gingiva. Once these tissues are lost, the foremost goal of periodontal therapy is to regenerate the diseased tissues if possible to their original form, architecture, and function. Various regenerative procedures were employed and still a gap was found in achieving the goal. As stem cells are characterized by their ability to self-renew and differentiate to produce specialized cells, there could be a possibility of using them for regenerative therapy. Recently, dental tissues such as the PDL, the dental pulp and the tooth follicle have been recognized as readily available sources of adult stem cells.AimThe aim was to identify the various sources and methodologies in isolation of stem cells from human oral cavity and its differentiation into various lineages using markers.Materials and MethodsThe electronic databases PUBMED, GOOGLE SCHOLAR, SCIENCE DIRECT, COCHRANE LIBRARY along with a complimentary manual search of all periodontics journal till the year 2016. Thirteen articles were selected on the basis of the inclusion criteria. Isolation of stem cells from oral cavity through various methods has been evaluated and similarly characterization to different lineages were tabulated as variables of interest. They included human in-vitro and ex-vivo studies.ResultsThe results showed that PDLSC’s and pulpal stem cells are the most common source from where stem cells were isolated. Each source has used different methodology in isolating the stem cells and it was found that STRO-1 was the commonly used marker in all the studies mentioned.ConclusionsThe studies showed that there is no standard protocol existed in isolating the stem cells from different sources of oral cavity. Moreover, there was no standard marker or methodology used in characterization.

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“…10 STRO-1-positive cells in human bone marrow have the capacity to self-renew and to differentiate into smooth muscle cells, adipocytes, chondrocytes, and osteoblasts. 10 Positive STRO-1 staining may indicate secretion of bone matrix and of cementoid and dentinoid material. 19 STRO-1 has been associated with increased osteogenic capacity 20 and is a marker of stem cells.…”

Section: Discussionmentioning

confidence: 99%

The role of Osterix protein in the pathogenesis of peripheral ossifying fibroma

et al. 2017

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Peripheral ossifying fibroma (POF) is a reactive lesion of oral tissues, associated with local factors such as trauma or presence of dental biofilm. POF treatment consists of curettage of the lesion combined with root scaling of adjacent teeth and/or removal of other sources of irritants. This study aimed to analyze the clinical and pathological features of POF and to investigate the immunoexpression of Osterix and STRO-1 proteins. Data such as age, gender, and size were obtained from 30 cases of POF. Microscopic features were assessed by conventional light microscopy using hematoxylin-eosin staining and immunohistochemical markers, and by polarized light microscopy using Picrosirius red staining. The age range was 11-70 years and 70% of the patients were female. Moreover, the size of POF varied from 0.2 to 5.0 cm; in 43.33% of the cases, the mineralized content consisted exclusively of bony trabeculae. The immunohistochemical analysis showed nuclear staining for Osterix in 63% and for STRO-1 in 20% of the cases. Mature collagen fibers were observed in mineralized tissue in 76.67% of the cases. The clinical and microscopic features observed were in agreement with those described in the literature. Osterix was overexpressed, while STRO-1 was poorly expressed. Osterix was expressed particularly in cells entrapped in and around mineralized tissue, indicating the presence of a stimulus that triggers the differentiation of these cells into osteoblasts or cementoblasts, i.e., cells that produce mineralized tissue. Based on our results, Osterix may play a role in the pathogenesis of POF.

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A Synthetic Oligopeptide Derived From Enamel Matrix Derivative Promotes the Differentiation of Human Periodontal Ligament Stem Cells Into Osteoblast‐Like Cells With Increased Mineralization

Abstract: SP enhances the formation of calcified nodules and osteocalcin production in the culture of PDLSCs into osteoblast-like cells and is a useful material for periodontal tissue regeneration.

Cited by 35 publications

References 40 publications

The Effects of Synthetic Oligopeptide Derived from Enamel Matrix Derivative on Cell Proliferation and Osteoblastic Differentiation of Human Mesenchymal Stem Cells

The Effects of Synthetic Oligopeptide Derived from Enamel Matrix Derivative on Cell Proliferation and Osteoblastic Differentiation of Human Mesenchymal Stem Cells

Methods of Isolation and Characterization of Stem Cells from Different Regions of Oral Cavity Using Markers: A Systematic Review

The role of Osterix protein in the pathogenesis of peripheral ossifying fibroma

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