A Survey of β-Lactamase-Producing &lt;i&gt;Escherichia coli&lt;/i&gt; in Farm Animals and Raw Retail Meat in Shizuoka Prefecture, Japan

Hiroi, Midori; Harada, Taishi; Kawamori, Fumihiko; Takahashi, N.; Kanda, Takashi; Sugiyama, Ken; Masuda, Takashi; Yoshikawa, Yuko; Ohashi, Norio

doi:10.7883/yoken.64.153

Cited by 27 publications

(4 citation statements)

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“…All 49 3GC-or 4GCresistant APEC isolates possessed one or more β-lactamase genes. In this study, the most prevalent ESBL-encoding genes were CTX-M group I and CTX-M group IV, which is similar to the previous findings in poultry and humans worldwide (31,(45)(46)(47). CTX-M types are often associated with coresistance phenotypes-particularly resistant to fluoroquinolones and aminoglycosides (48).…”

Section: Discussionsupporting

confidence: 91%

Genetic characterization of third- or fourth-generation cephalosporin-resistant avian pathogenic Escherichia coli isolated from broilers

Kang

Lim²,

Lee³

2022

Front. Vet. Sci.

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The third- or fourth-generation cephalosporins (3GC or 4 GC) are classified as “critically important antimicrobials for human medicine” by WHO, but resistance to these drugs is increasing rapidly in avian pathogenic E. coli (APEC). This study investigated the distribution and genetic characteristics of 3GC- or 4 GC-resistant APEC isolates from five major integrated broiler operations in Korea. The prevalence of 3GC- or 4GC-resistant APEC isolates in 1-week-old broilers was the highest in farms of operation C (53.3%); however, the highest prevalence of these isolates in 4-week-old broilers was the highest on the farms of operation A (60.0%), followed by operations E (50.0%) and C (35.7%). All 49 3GC- or 4GC-resistant APEC isolates had at least one β-lactamase-encoding gene. The most common β-lactamase-encoding genes was extended-spectrum β-lactamase gene, blaCTX−M−15, detected in 24 isolates (49.0%), followed by blaTEM−1 (32.7%). Sixteen isolates (32.7%) harbored class 1 integrons, and four isolates (8.2%) showed different gene cassette-arrangements. However, only 1 of 26 isolates harboring class 2 integrons carried a gene cassette. Furthermore, both CRISPR 1 and 2 arrays were detected in most isolates (36 isolates; 73.5%), followed by CRISPR 2 (18.4%) and CRISPR 1 (4.1%). Interestingly, CRISPR 2 was significantly more prevalent in multidrug resistant (MDR)-APEC isolates than in non-MDR APEC isolates, whereas CRISPR 3 and 4 were significantly more prevalent in non-MDR APEC isolates (each 11.1%; p < 0.05). None of the protospacers of CRISPR arrays were directly associated with antimicrobial resistance. Our findings indicate that the distribution and characteristics of 3GC or 4GC-resistant APEC isolates differed among the integrated broiler operations; moreover, improved management protocols are needed to control the horizontal transmission of 3GC or 4GC-resistant APEC isolates.

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Section: Discussionsupporting

confidence: 91%

Genetic characterization of third- or fourth-generation cephalosporin-resistant avian pathogenic Escherichia coli isolated from broilers

Kang

Lim²,

Lee³

2022

Front. Vet. Sci.

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“…(71% vs 95%), and S. aureus (87% vs none) were reported in cloacal swab and sewage samples, respectively 21 , 26 , 30 , 31 . The variable occurrence of E. coli (9.2–84%) in cloacal swab samples was reported earlier in Egypt (9.2%) 20 , Japan (84%) 32 , Portugal (42.1%) 19 , and Belgium (60.3%) 33 . Besides, the predominance of Salmonella spp.…”

Section: Discussionsupporting

confidence: 65%

Sink survey to investigate multidrug resistance pattern of common foodborne bacteria from wholesale chicken markets in Dhaka city of Bangladesh

Parvin

Ali

Mandal

et al. 2022

Sci Rep

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Antimicrobial resistance (AMR) among foodborne bacteria is a well-known public health problem. A sink survey was conducted to determine the AMR pattern of common foodborne bacteria in cloacal swab of broiler chickens and sewage samples from five wholesale chicken markets of Dhaka city in Bangladesh. Bacteria were identified by culture-based and molecular methods, and subjected to antimicrobial susceptibility testing. Resistance genes were identified by multiplex PCR and sequencing. Multidrug resistance (MDR) was observed in 93.2% of E. coli, 100% of Salmonella spp., and 97.2% of S. aureus from cloacal swab samples. For sewage samples, 80% of E. coli, and 100% of Salmonella and S. aureus showed MDR. Noteworthy, 8.3% of S. aureus from cloacal swab samples showed possible extensively drug resistance. Antimicrobial resistance genes (beta-lactamase—blaTEM, blaSHV; quinolone resistance gene—qnrS) were detected in a number of E. coli and Salmonella isolates from cloacal swab and sewage samples. The methicillin resistance gene (mecA) was detected in 47.2% and 25% S. aureus from cloacal swab and sewage samples, respectively. The findings envisage the potential public health risk and environmental health hazard through spillover of common foodborne MDR bacteria.

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“…Country-specific findings indicated that Nigeria had a PPE of 33.4% and 11.9% for E. coli and K. pneumoniae , respectively. Other countries, including Sudan [ 29 ], India [ 9 ], Algeria [ 30 ], Ghana [ 31 ], Turkey [ 32 ], Japan [ 33 ], Canada [ 34 ], Finland [ 35 ], Taiwan [ 36 ], South Africa [ 37 ], Tanzania [ 38 ], Tunisia [ 39 ], India [ 40 ], and the Netherland [ 41 ] were not included in the meta-analysis due to the low number of studies.…”

Section: Discussionmentioning

confidence: 99%

“One Health” perspective on prevalence of co-existing extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae: a comprehensive systematic review and meta-analysis

Ramatla,

Mafokwane,

Lekota

et al. 2023

Ann Clin Microbiol Antimicrob

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Background The Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) bacterial isolates that produce extended-spectrum β-lactamases (ESBLs) contribute to global life-threatening infections. This study conducted a systematic review and meta-analysis on the global prevalence of ESBLs in co-existing E. coli and K. pneumoniae isolated from humans, animals and the environment. Methods The systematic review protocol was registered in the International Prospective Register of Systematic Reviews (PROSPERO) [ID no: CRD42023394360]. This study was carried out following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. One hundred and twenty-six eligible studies published on co-existing antibiotic resistance in E. coli and K. pneumoniae between 1990 and 2022 were included. Results The pooled prevalence of ESBL-producing E. coli and K. pneumoniae was 33.0% and 32.7% for humans, 33.5% and 19.4% for animals, 56.9% and 24.2% for environment, 26.8% and 6.7% for animals/environment, respectively. Furthermore, the three types of resistance genes that encode ESBLs, namely blaSHVblaCTX−M,blaOXA, and blaTEM, were all detected in humans, animals and the environment. Conclusions The concept of “One-Health” surveillance is critical to tracking the source of antimicrobial resistance and preventing its spread. The emerging state and national surveillance systems should include bacteria containing ESBLs. A well-planned, -implemented, and -researched alternative treatment for antimicrobial drug resistance needs to be formulated.

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A Survey of β-Lactamase-Producing <i>Escherichia coli</i> in Farm Animals and Raw Retail Meat in Shizuoka Prefecture, Japan

Cited by 27 publications

References 0 publications

Genetic characterization of third- or fourth-generation cephalosporin-resistant avian pathogenic Escherichia coli isolated from broilers

Genetic characterization of third- or fourth-generation cephalosporin-resistant avian pathogenic Escherichia coli isolated from broilers

Sink survey to investigate multidrug resistance pattern of common foodborne bacteria from wholesale chicken markets in Dhaka city of Bangladesh

“One Health” perspective on prevalence of co-existing extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae: a comprehensive systematic review and meta-analysis

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