A survey of the occurrence and properties of methicillin‐resistant Staphylococcus aureus and methicillin‐resistant Staphylococcus intermedius in water buffalo milk and dairy products in Turkey

Abstract

Antimicrobial resistance, β‐lactamase activity and mecA gene of Staphylococcus aureus and Staphylococcus intermedius isolated from raw water buffalo milk and dairy products in Turkey were determined. All strains showed resistance to at least one antibiotic but none was resistant to vancomycin. Of the 97 S. aureus and 35 S. intermedius strains, 9 (9.2%) and 2 (5.7%) were resistant to oxacillin and harboured mecA gene. β‐lactamase activity of 13.4% and 5.7% of S. aureus and S. intermedius strains was positive, respectively. Overall, 2.5% and 0.55% of the samples were contaminated with methicillin‐resistant S. aureus and S. intermedius, respectively.

Detection of Enterotoxin Genes and Methicillin‐Resistance in Staphylococcus aureus Isolated from Water Buffalo Milk and Dairy Products

“…Similar to the findings of our study, a study conducted in Afyon, Turkey, by Pamuk et al. () reported that out of 360 buffalo milk and milk products samples, 9 (9.2%) of the 97 S. aureus isolates were genetically carrying mecA gene. In contrast to our study, Nam et al.…”

“…The results of our study are similar to those of other researchers who found a S. aureus prevalence of 28% to 33% in buffalo milk (Abo-Shama, 2014;Pamuk, Yildirim, Seker, Gurler, & Kara, 2012;Sharma, Sharma, & Malik, 2011;Singh & Prakash, 2010). In another study conducted by Alisarli and Solmaz (2010), S. aureus was detected in 38% of teat skin samples.…”

Investigation of mecA gene, virulence traits and antibiotic resistance profiles in methicillin‐resistant Staphylococcus aureus isolates from dairy products

“…The same result was found by Ektik et al (), and similar results were published by Papadopoulos et al () with 99.3% resistance and by Can et al () with 95% resistance. Consistent with our data, several studies performed in different countries also reported resistance to tetracycline (Can & Çelik, ; Ferreira et al, ; Oniciuc et al, ; Pamuk et al, ; Papadopoulos et al, ; Traversa et al, ), clindamycin (Can & Çelik, ; Pamuk et al, ; Traversa et al, ), and chloramphenicol (Pamuk et al, ; Papadopoulos et al, ). In addition to antibiotics tested in the present study, resistance to erythromycin (Can & Çelik, ; Pamuk et al, ; Traversa et al, ), ampicillin (Can & Çelik, ; Ektik et al, ), and ciprofloxacin (Oniciuc et al, ; Papadopoulos et al, ) has been reported in milk and dairy isolates of MRSA.…”

“…Studies performed during the last decade in different countries indicated the presence of mec A‐positive and/or phenotype‐positive MRSA (Basanisi, La Bella, Nobili, Franconieri, & La Salandra, ; Carfora et al, ; Kamal, Bayoumi, & Abd El Aal, ; Song et al, ; Traversa et al, ) in milk. The occurrence of MRSA in milk was also reported by researches from Turkey (Buyukcangaz et al, ; Pamuk, Yildirim, Seker, Gurler, & Kara, ; Siriken, Yildirim, Güney, Erol, & Durupinar, ). Contrary to our results, Oniciuc et al (), Can, Elmalı, and Karagöz (), and Papadopoulos et al () did not find the mec A gene or resistance to methicillin in milk isolates of S. aureus .…”

“…Vancomycin resistance in food isolates of MRSA or methicillin‐susceptible S. aureus was studied by many researchers in Turkey, but neither was detected (Aydin, Sudagidan, & Muratoglu, ; Can et al, ; Pamuk et al, ). Conversely, we observed that one MRSA strain (with a high oxacillin MIC of 256 μg/ml) isolated from buttercream had vancomycin resistance with an MIC of 256 μg/ml.…”