Occurrence and characteristics of methicillin-resistant Staphylococcus aureus (MRSA) in buffalo bulk tank milk and the farm workers in Italy

Normanno, G.; Spinelli, Elisa; Caruso, Marta; Fraccalvieri, Rosa; Capozzi, Loredana; Barlaam, Alessandra; Parisi, Antonio

doi:10.1016/j.fm.2020.103509

Cited by 14 publications

(8 citation statements)

References 50 publications

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“…In a previous study, 2 of the isolated S. aureus strains from cow milk were vancomycin-resistant (Kou et al, 2021). Inconsistent with the present study, all of the previously isolated S. aureus strains from cheese and milk were sensitive to vancomycin (Castro et al, 2020;Giovanni et al, 2020).…”

Section: Discussionsupporting

confidence: 85%

“…In addition, 51.6% of the isolated S. aureus strains from milk samples were identified as MRSA strains (Kou et al, 2021) which is nearly similar to the present study. However, 5.4% of MRSA levels were noticed in the buffalo bulk tank milk in Italy which is lower than the present study (Giovanni et al, 2020). Further, 100% of oxacillin resistant S. aureus strains isolated from milk and cheese samples, were mecA gene positive confirming the presence of MRSA isolates (Zayda et al, 2020).…”

Section: Discussioncontrasting

confidence: 71%

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Phenotypic and Genotypic Characterization of Methicillin, Vancomycin, and Erythromycin-resistant Staphylococcus aureus Isolated from Milk and Dairy Products

Omara¹,

Hakim²,

Bakry³

2021

WVJ

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Detailed information on the resistance patterns of Staphylococcus aureus (S. aureus) in milk and cheese is strongly required to facilitate risk assessment analysis in case of food poisoning context and to improve therapeutic approaches used in dairy farms. The present study aimed to perform phenotypic and genotypic antimicrobial characterizations of methicillin, vancomycin, and erythromycin-resistant S. aureus isolated from milk and dairy products through screening mecA, vanA, and ermC using molecular PCR amplification technology. Moreover, the association between each genotypic and its related antibiotic resistance phenotypic features within the isolated S. aureus strains were analyzed. Moreover, the current study aimed to study MRSA's ability to form biofilms. Out of 226 milk and dairy product samples collected from different retailers in Giza Governorate, 69.5% of the samples were positive for the presence of S. aureus. The isolation rate of S. aureus strains from cattle milk, sheep milk, white cheese, flamenco, and mesh samples were 79.7%, 76.5%, 56.0%, 40.0%, and 94.7%, respectively. Multidrug-resistant S. aureus (MDR) was detected in 51% of all isolated S. aureus strains. All tested S. aureus strains were sensitive to trimethoprim-sulfamethoxazole, linezolid, ciprofloxacin, and gentamycin. However, their resistance rates against penicillin, oxacillin, vancomycin, erythromycin, tetracycline, clindamycin and chloramphenicol were 62.4%, 65.0%, 44.6%, 45.9%, 21.0%, 14.0%, and 2.5%, respectively. Of the isolated S. aureus strains, 72.6%, 40.1%, and 48.4% were carriers for mecA, vanA, and ermC genes and the amplified products were at 310, 1030, and 295 bp, respectively. Methicillin-resistant S. aureus isolates were detected in 47.1% of all isolated S. aureus strains. The results indicated that 35.0% of the tested S. aureus strains were genotypic vanA gene carriers and phenotypic resistant to vancomycin representing vancomycin-resistant S. aureus strains. Moreover, 42.7% of all isolated S. aureus strains were carriers for ermC gene and were phenotypic resistant to erythromycin representing erythromycin-resistant S. aureus. The presence of mecA, vanA, and ermC genes in S. aureus was statistically associated with their related phenotypic resistance patterns against both penicillin and oxacillin, vancomycin, and erythromycin, respectively. Moreover, along with an increase in the frequency of mecA, vanA, and ermC genes, their phenotypic antibiotic resistance patterns sharply increased with an odd ratio >1. Of MRSA isolates, 6.8% indicated weak biofilm-formation ability, while 93.2% exhibit no biofilm-forming ability.

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Section: Discussionsupporting

confidence: 85%

Section: Discussioncontrasting

confidence: 71%

Phenotypic and Genotypic Characterization of Methicillin, Vancomycin, and Erythromycin-resistant Staphylococcus aureus Isolated from Milk and Dairy Products

Omara¹,

Hakim²,

Bakry³

2021

WVJ

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“…The number of isolates collected and analysed within each study ranged from 3 [36] to 3006 [10]. The percentage of MDR detected in relation to the total number of samples analysed ranged from 3%, found by Mascaro et al in a group of sheep farmers [27], to 78%, found by Pompilio et al in a hospital environment [38], excluding studies that only analysed MDR which therefore found a resistance rate of 100% (for K. Pneumoniae [15,17,36,40,43], A. baumanii [12], H. pylori [42], S. aureus [45]).…”

Section: Multidrug-resistant Microorganismsmentioning

confidence: 97%

“…Overall, the studies included in the review analysed more than 30 different microorganisms. In the study by La Fauci et al [30] as many as twelve different microorganisms were taken into account, whereas in most studies (n = 23, 53.5%) only one microorganism was analysed [12,13,15,21,23,25,27,29,31,[33][34][35][36][37][38][39][40][41][42][44][45][46]50].…”

Section: Multidrug-resistant Microorganismsmentioning

confidence: 99%

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Antimicrobial Resistance (AMR) in Italy over the Past Five Years: A Systematic Review

et al. 2022

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Antimicrobial Resistance (AMR) has become a global threat to public health systems around the world in recent decades. In 2017, Italy was placed among the worst-performing nations in Europe by the European Centre for Disease Prevention and Control, due to worryingly high levels of AMR in Italian hospitals and regions. The aim of this systematic review was to investigate the state of the art of research on AMR in Italy over the last five years. The PubMed database was searched to identify studies presenting original data. Forty-three of the 9721 records identified were included. Overall, AMR rates ranged from 3% (in a group of sheep farmers) to 78% (in a hospital setting). The methods used to identify the microorganisms, to test their susceptibility and the criteria adopted for the breakpoint were deficient in 7, 7 and 11 studies, respectively. The main findings of our review were that most studies (79.1%) considered hospitalised patients only, 4 studies (9.3%) analysed non-hospitalised populations only. In addition, only 7 studies were multicentric and no scientific literature on the subject was produced in 7 Italian regions. In order to have a solid basis on the topic for the interventions of public health professionals and other stakeholders, studies analysing the phenomenon should be conducted in a methodologically standardised manner, should include all areas of the country and should also focus on out-of-hospital and community-based care and work settings.

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Bringing resistance modulation to epidemic methicillin resistant S. aureus of dairy through antibiotics coupled metallic oxide nanoparticles

Lodhi

Saleem

Aqib

et al. 2021

Microbial Pathogenesis

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Occurrence and characteristics of methicillin-resistant Staphylococcus aureus (MRSA) in buffalo bulk tank milk and the farm workers in Italy

Cited by 14 publications

References 50 publications

Phenotypic and Genotypic Characterization of Methicillin, Vancomycin, and Erythromycin-resistant Staphylococcus aureus Isolated from Milk and Dairy Products

Phenotypic and Genotypic Characterization of Methicillin, Vancomycin, and Erythromycin-resistant Staphylococcus aureus Isolated from Milk and Dairy Products

Antimicrobial Resistance (AMR) in Italy over the Past Five Years: A Systematic Review

Bringing resistance modulation to epidemic methicillin resistant S. aureus of dairy through antibiotics coupled metallic oxide nanoparticles

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