A Study on Aging Kinetics, Inhibition, and Reactivation of Organophosphate Compounds with Quails and Chicks Carboxylesterase

Abass, Kasim Sakran

doi:10.1080/00387010.2013.874359

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…For example, the process of hydrolytic decomposition of ACh in the nervous system by AChE can accelerate the assembly of amyloid ␤ peptides into amyloid fibrils, resulting in Alzheimer's disease (AD) [6][7][8]. Therefore, current rational pharmaceutical treatment of AD is clinically applied to increase the concentration of ACh [9].…”

Section: Introductionmentioning

confidence: 99%

Colorimetric assay for acetylcholinesterase and inhibitor screening based on the Ag [I] ion–3,3′,5,5′-tetramethylbenzidine (TMB)

Sun

Dai

et al. 2016

Sensors and Actuators B: Chemical

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Section: Introductionmentioning

confidence: 99%

Colorimetric assay for acetylcholinesterase and inhibitor screening based on the Ag [I] ion–3,3′,5,5′-tetramethylbenzidine (TMB)

Sun

Dai

et al. 2016

Sensors and Actuators B: Chemical

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“…Cholinesterases activities were determined at room temperature 25°C by the Ellman method [15] and different thiocholine substrates (AβMSCh, BuSCh, and PrSCh) were used for measuring enzymes activity. Following combination of thiocholine derivatives with 5, 5′-dithiobis (2-nitrobenzoic acid) forms the yellow anion 5-thio-2-nitrobenzoic acid, which absorbs strongly at 412 nm [16]. Substrate solutions were prepared and used on the same day and kept on ice during use.…”

Section: Enzyme Analysesmentioning

confidence: 99%

Cholinesterase Effects of Pesticides in Rabbit Embryos and their Potential for Use in Hazard Assessment

Abass¹,

Salih²

2016

J Vet Sci Technol

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Cholinesterase are often taken as biomarkers of exposure and effect. Then acetylhydrolase isozymes and acylcholine acylhydrolase were examined for their suitability as biomarkers of pesticide exposure. The study of effects of embryos of the rabbit is already an accepted tool in monitor food waste levels, but effects of various groups of substances to the rabbit embryo remain to be studied. A 43% inhibition of acetylhydrolase enzyme activities was reached by 250 mg/kg malathion, while 35% inhibition of acylcholine acylhydrolase enzyme activities was reached by 500 mg/kg malathion indicating the possible use of these enzymes as biomarkers of exposure. There are found some significant specific activities of the acetylhydrolase ranged from 1.5-7.1 U mg −1 protein. The specific activity of acylcholine acylhydrolase in the range of 0.9-4.5 U mg −1 protein in the respective developmental stages. Substratespecificity was analyzed using specific inhibitors (iso-OMPA and BW284c51). The results showed that the observed cholinesterase's activities in the whole embryo may be attributed mainly to acetylhydrolase with a partial capability to use butyrylthiocholine (BuSCh) as an additional substrate.

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A Study on Aging Kinetics, Inhibition, and Reactivation of Organophosphate Compounds with Quails and Chicks Carboxylesterase

Cited by 2 publications

References 26 publications

Colorimetric assay for acetylcholinesterase and inhibitor screening based on the Ag [I] ion–3,3′,5,5′-tetramethylbenzidine (TMB)

Colorimetric assay for acetylcholinesterase and inhibitor screening based on the Ag [I] ion–3,3′,5,5′-tetramethylbenzidine (TMB)

Cholinesterase Effects of Pesticides in Rabbit Embryos and their Potential for Use in Hazard Assessment

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