A Study of the Serological Behaviour and Nature of the
Anti-B/P/P^k Activity of Salmonidae Roe Protectins

Voak, D.; Todd, G.M.; Pardoe, G.I.

doi:10.1159/000467747

Cited by 11 publications

(17 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous efforts to find a non-human source of anti-B have shown that plant [2,3,12,19,21] and fish [1,6,18,20,23] anti-B agglutinins lack either the activity, specificity or stability of human typing se…”

Section: Introductionmentioning

confidence: 99%

Monoclonal Anti-B as a New Blood-Typing Reagent

Sacks

Lennox²

1981

Vox Sanguinis

View full text Add to dashboard Cite

Stable cloned lines of anti-B-secreting cells have been derived by fusing spleen cells of a mouse immunized by group B antigen with a mouse myeloma line. The tissue culture supernatant of one of them (NB1/19.112.28) containing secreted monoclonal anti-B antibody is an especially good blood-grouping reagent, well suited for use with manual and automated methods. The unlimited availability of a potent reagent of uniform properties, cheaply produced by a single source, makes monoclonal anti-B a serious competitor to human typing serum.

show abstract

Section: Introductionmentioning

confidence: 99%

Monoclonal Anti-B as a New Blood-Typing Reagent

Sacks

Lennox²

1981

Vox Sanguinis

View full text Add to dashboard Cite

show abstract

“…However, recently we observed [16] slightly greater activity with AjP^ than AjPj or AjP2 red cells with salmon 1 Plk=P1+Pk, P^Pj+P and P2=P.…”

mentioning

confidence: 69%

The α-Galactose Specificity of Anti-P^k

Voak¹,

Anstee²,

Pardoe³

1973

Vox Sanguinis

Self Cite

View full text Add to dashboard Cite

These studies indicate that α-galactose is the terminal and immunodominant sugar of the Pk determinant. Human anti-P^k and the ‘anti-P^k’ activity of salmon and trout protectins are strongly inhibited by α-galactosyl groups of galactose, disaccharides and macromolecules, e.g. P(1) substance. Removal of α-galactose from P(1) substance abolishes its ability to inhibit anti-P^k antibody activity. Anti-P^k antibody has a narrow spectrum for terminal α-galactosyl groups of cell surface antigens defining those of P^k but not those of B, P(1) or P(2) cells, while all these cells are agglutinated by the fish roe protectins. We suggest that anti-P^k antibody selects a larger determinant than the terminal α-galactosyl group and the full determinant is structurally different from those of B, P(1) and P(2) antigens. By contrast, fish roe agglutinins bind to a part only of the α-galactosyl group, present in all these antigens.

show abstract

“…There are fewer examples of B-specific lectins. As the specificity of these lectins depends on the terminal D-galactose determinant, they may cross-react with other antigens containing similar residues, i.e P, P1 and P k (Voak et al, 1974). The most used reagent to detect secretor status in saliva from O group individuals is Ulex europaeus lectins which recognize the H antigen.…”

Section: Abo Typingmentioning

confidence: 99%